Artificial hybrid breeding method of Primula L.

A technology of artificial hybridization and primrose, which is applied in the field of artificial hybridization of primrose, can solve the problems of tediousness, new variety specificity, consistency, unsatisfactory stability, and high technical requirements for implementation, and achieve the goal of ensuring germination rate Effect

Active Publication Date: 2013-05-01
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most flower enterprises in China mainly rely on imported first-generation primrose hybrid seeds for production. In traditional breeding methods, continuous multi-generation self-crossing methods can effectively obtain homozygous traits with stable inheritance. Due to its self-incompatibility Pollination at bud stage, excision of stigma, pollination in ovary, in vitro fertilization, phytohormone treatment of pistil and other methods can be used to overcome self-incompatibility, so that plants can obtain inbred lines, but the implementation of these methods requires high technical requirements And cumbersome, the specificity, consistency and stability of the new varieties obtained are not satisfactory

Method used

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Effect test

Embodiment 1

[0032] In mid-July, 2010, an artificial cross experiment was carried out with Primula beesiana as the female parent and Primula bulleyana as the male parent. There are 4 treatments in total: a) Xiahong Dengtai Primula (pin)♀×Orange Red Dengtai Primula (pin)♂; b) Xiahong Dengtai Primula (pin)♀×Orange Red Dengtai Primula (thrum)♂; c) Xiahong Dengtai Primula (thrum) ♀ × Orange Red Dengtai Primula (pin) ♂; d) Xiahong Dengtai Primula (thrum) ♀ × Tangerine Thrum (thrum) ♂.

[0033] Pollen collection of Primula tangerina: Due to the overlapping flowering periods of the two parents, the pollen of Primula tangerine can be taken and used immediately.

[0034] Detasseling of Xiahong Dengtai Primula: On July 10, select the inflorescences of the soon-to-open Xiahong Dengtai Primula and bag them, and mark them with tweezers; then bag them. When all the treated flowers opened on the 12th, the hybrid pollination experiment was carried out. The criterion for judging that the stigma is pollin...

Embodiment 2

[0040] In mid-July, 2010, an artificial cross experiment was carried out with Primula bulleyana as the female parent and Primula beesiana as the male parent during the full flowering stage. There are 4 treatments in total: namely a) orange-red lampstand spring (pin)♀×Xiahong lampstand primula (pin)♂; b) orange-red lampstand primula (pin)♀×xiahong lampstand spring (thrum)♂; c) orange-red lampstand thrum (thrum)♀×xiahong-red lampstand-primula (pin)♂; d) orange-red lampstand primula (thrum)♀×xia-red lampstand primula (thrum)♂.

[0041] Pollen collection of Primula xiahongdengtai: Due to the overlapping flowering periods of the two parents, the pollen of Primula xiahongdengtai can be taken and used immediately.

[0042] Primula detasseling of orange-red lampstands: On July 10, select the inflorescences of the soon-to-open Primula orange-red lampstands and bag them, and mark them with tweezers; then bag them. When all the treated flowers opened on the 12th, the hybrid pollination ...

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Abstract

The invention belongs to the biotechnical field, and concretely discloses an artificial hybrid breeding method of Primula L.. The method comprises the following steps: carrying out artificial hybrid pollination of the respective long-style flowers and short-style flowers of Primula bulleyana and Primula beesiana which are Primula plants, well worth appreciating and are adopted male and female parents; and carrying out embryo abortion detection of the hybrid F1 seeds through a digital X-ray sample imaging system, selecting seeds having normal embryonic development, disinfecting the seeds, germinating on a culture medium, carrying out hardening seedling, and transplanting. The method realizes the distant hybirdization of the Primula bulleyana and the Primula beesiana, enables the scientific detection of the seed embryo abortion to be carried out through utilizing the digital X-ray sample imaging system, and allows new species of Primula L. having more ornamental values to be cultivated.

Description

Technical field: [0001] The invention relates to a method for hybrid breeding of plants, in particular to a method for artificial hybrid breeding of primroses. Background technique: [0002] Primula (Primula L.) is a famous ornamental flower in the world. It is favored by horticulturalists because of its bright colors and high ornamental value. It is known as one of the "three major horticultural plants in the world". Section Proliferae Pax is one of the most popular groups of horticulturalists in the Primula genus. Primula beesiana and Primula bulleyana are perennial herbaceous flowers with beautiful flowers and strong adaptability ( figure 1 ). The corolla of Xiahong Primula is purple, and the corolla of Orange-red Primula is yellow. It is a typical self-incompatibility (self-incompatibility) plant. Long-term cross-pollination makes the characters of offspring widely separated, and the traits such as flower type and flower color A series of variations are difficult to s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 马永鹏罗桂芬吴之坤田小玲薛瑞娟
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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