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Diabetes type II gene susceptivity and pre-warning detection kit

A detection kit and technology for diabetes, applied in the field of type 2 diabetes genetic susceptibility and early warning detection kits, can solve the problems of decreased insulin secretion, affected beta cell secretion function, increased risk of type 2 diabetes, etc.

Inactive Publication Date: 2013-05-01
SHANGHAI JINFANG BIOLOGICAL TECH
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  • Claims
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AI Technical Summary

Problems solved by technology

If rs10811661 near the CDKN2A / 2B gene mutates, resulting in overexpression of the CDKN2A / 2B gene product, it will significantly inhibit CDK4 activity, further affect the secretion function of β cells, reduce insulin secretion, and increase the risk of type II diabetes

Method used

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  • Diabetes type II gene susceptivity and pre-warning detection kit
  • Diabetes type II gene susceptivity and pre-warning detection kit
  • Diabetes type II gene susceptivity and pre-warning detection kit

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Embodiment Construction

[0032] The present invention will be further described below in conjunction with specific examples, and the examples are only for the purpose of illustration, and are not intended to limit the scope of the present invention. The experimental conditions and experimental methods that are not described in detail and specifically described in the following examples should be conventional experimental conditions and methods, or be carried out according to the operating steps and methods provided by the manufacturer.

[0033] Embodiment The use of this kit

[0034] 1. Obtaining the genomic DNA of the sample: rinse the mouth with water once or twice to remove food residues in the mouth, and pay attention to swallowing the remaining water in the mouth. Use a cotton swab to scrape off the exfoliated cells of the oral mucosa and put them into the cell fixative in an Eppendorf tube, and tighten the bottle cap for later use.

[0035] 2. DNA extraction and purification: use the cell lysis...

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Abstract

The present invention provides a diabetes type II gene susceptivity and pre-warning detection kit, which is characterized in that seven nucleic acid (DNA and RNA) molecule markers are combined, wherein the seven nucleic acid (DNA and RNA) molecule markers comprise APM1 (+45), CDKAL1 (rs10946398), TCF7L2 (rs7903146) , CDKN2A / 2B (rs10811661), KCNQ1 (rs2237892), VDR (FokI) and SLC30A8 (rs13266634). The kit comprises: specific amplification primer pairs and DNA sequencing primer pairs of the gene polymorphism loci, PCR reaction liquids, PCR product enzymolysis liquids and DNA sequencing reaction liquids. According to the present invention, detection is performed by using the kit, gene information is processed and analyzed, diabetes type II susceptivity of a healthy subject is evaluated, and guiding significance is provided for personalized treatment and health care of diabetes type II people.

Description

technical field [0001] The invention relates to the technical fields of genetics, molecular biology, clinical medicine and preventive medicine. Specifically, the invention relates to a type II diabetes gene susceptibility and early warning detection kit, which is characterized in that multiple nucleic acids (DNA and RNA) molecular markers, including: gene polymorphisms on APM1(+45), CDKAL1(rs10946398), TCF7L2(rs7903146), CDKN2A / 2B(rs10811661), KCNQ1(rs2237892), VDR(Fok I), SLC30A8(rs13266634) The site, after the calculation analysis of the risk assessment model, can assess and screen the susceptibility of II diabetes of the subject. Background technique [0002] In 2000, there were 151 million diabetic patients in the world, and currently there are 285 million diabetic patients in the world. According to the current growth rate, it is estimated that the worldwide diabetic population will increase to 500 million by 2030, of which the developed countries will increase from 51 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 崔春黎肖自力纪燕燕
Owner SHANGHAI JINFANG BIOLOGICAL TECH
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