Applications of Chinese starjasmine stem aglycon, burdock aglycon and Chinese starjasmine stem glucoside as dibenzyl butyrolactone compounds to preparation of medicaments for treating hepatitis viruses
A technology for arctigenin and trephine aglycone, which is applied in the application, food preparation, antiviral agent and other directions, can solve the problems such as no anti-hepatitis C virus effect, etc., and achieve a remarkable anti-hepatitis C virus activity. Effect
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[0028] Example 1 Anti-hepatitis C virus test of strogenin, arctigenin and stroganoside
[0029] 1. Experimental drugs, reagents and materials
[0030] 1. Compounds: strogenin, arctigenin, strogenin.
[0031] 2. Cell line Huh7, human liver cancer cell line (see: Yimin Tong, Yongzhe Zhu, Xueshan Xia, Yuan Liu, et al. Tupaia CD81, SR-BI, Claudin-1, and Occludin Support Hepatitis C Virus Infection, JOURNAL OF VIROLOGY, 2011; 85(6): 2793-2802; Jin Zhong, Pablo Gastaminza, Guofeng Cheng, et al. Robust hepatitis C virus infection in vitro, PNAS, 2005; 102(26):9294-9299).
[0032] 3. Cell culture medium, prepared, which contains 10% fetal bovine serum, 0.03% glutamine, non-essential amino acids, ampicillin and streptomycin 100U / ml, adjust the pH to 7.4.
[0033] 4. Cell digestion solution, prepared, containing 0.25% trypsin, prepared with phosphate buffer.
[0034] 5. HCVcc: cell-cultured infectious hepatitis C virus (see: Yimin Tong, Yongzhe Zhu, Xueshan Xia, Yuan Liu, et al. Tu...
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