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Soybean GmMADS2 gene and application thereof

A soybean and gene technology, applied to the soybean GmMADS2 gene and its application field, can solve the problems such as the easy cracking of soybean pods

Inactive Publication Date: 2013-05-08
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an important leguminous plant, soybean has always occupied a special and important position in the nutrition of humans and animals with its high protein and high oil content, but soybean pods are easy to crack in the later harvest process

Method used

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  • Soybean GmMADS2 gene and application thereof
  • Soybean GmMADS2 gene and application thereof
  • Soybean GmMADS2 gene and application thereof

Examples

Experimental program
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specific Embodiment approach （

[0027] Specific embodiments (the following combined diagram illustration results)

[0028] The following combined with the attachment and embodiments to further explain the present invention.

[0029] If there is no special description in the following examples, it is a conventional method.

Embodiment 1

[0030] Example 1. Soybean SHP Gene Gmmads2 CDNA clone and identification

[0031] Soybean (soybean ( Glycine Max (L.) Merr.) Jackson is provided by the Genoms of the National Soy Soy Soy Soy Soy Soy Modification Center of Nanjing Agricultural University. It is planted in the network room of Nanjing Agricultural University in early June and grows under natural conditions.Take the roots and leaves of the six compound leaves of the soybean, the soybean flowers (R1) to take the mixture of flowers and flower buds, and the pods of the first pod stage (R3) of the soybean.GMA.11881.1.a1_AT of the root and height of the roots and leaves, the results of the real-time quantitative RT-PCR also verified this.Through the BLAST program, we use GMA.11881.1.a1_AT as a probe sequence to search for soybean EST database, design primer according to the results of the sequence stitching, use the cdna of the mixture of the beans and flower buds as the template, and obtain 1 through the RT-PCR method to ...

Embodiment 2

[0036] Example 2, soybeans Gmmads2 The expression characteristics of genes in different tissues

[0037] The soybean variety Jackson was planted in the network room of Nanjing Agricultural University in early June, and it grew under natural conditions.When the third compound leaves are unfolded, the roots, stems, leaves, and stem tip are expanded; during the flowering period, the mixture of flowers and flower buds, the pods and pods of the first soybean pod stage (R3);Four -wheeled organ: calyx, petals, stamens, and pistils are quickly separated under sterile conditions; the seeds, cotyles and embryos are separated from 20 DAF seeds under aseptic conditions.After the materials are collected, the liquid nitrogen is frozen, and the -80 ° C is stored for later use.

[0038] The extraction of the total RNA with the embodiment 1.Expressed in the formation of soybeans ActinGene (Genbank Accession No.V00450) is an internal reference. The total RNA of different tissues from soybeans has b...

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Abstract

The invention belongs to the field of plant genetic engineering, and relates to a soybean GmMADS2 gene and an application thereof. The GmMADS2 gene which plays an important regulation and control role in controlling pod coat cracking and floral organ development is cloned from soybean, and the sequence of the GmMADS2 gene is shown in SEQIDNO.1. Expression analysis of an mRNA gene proves that the gene can participate in the development of pistil, pod and seed coat, and furthermore, reproductive organ variation of arabidopsis capable of over-expressing the GmMADS2 gene proves that the GmMADS2 gene plays a very important regulation and control role in controlling pod cracking and petal development. The invention discloses a genetic engineering improvement method for modifying the plant reproductive organ by the gene, and the method has a certain effect on the cultivation of a plant variety with varied reproductive organs, especially on the cultivation of the soybean, and serves for the improvement of the crop seed production efficiency by modifying flower organ forms and pod forms in an oriented manner to further serve for the increase of crop yield, especially the increase of soybean yield.

Description

Technical field [0001] The invention belongs to the field of plant genetic engineering, involving soybeans Gmmads2 Gene and application. Background technique [0002] SHP The gene is an embryonic characteristic gene, which has the function of the D -class gene. It plays an important role in the decisive decisions of the beads, the development of the heart skin, and the mature fruity and cracking.After the fruit is mature, it will crack, release the seeds, and the timely cracking is conducive to the collection of seeds. Premature cracking is not conducive to the harvest of agricultural products.As an important bean -based plant, soybeans have always occupied a special and important position in the nutrition of humans and animals in human and animals, but the soybean harvesting process is easy to crack pods.Nowadays, with the continuous development of soybean industrialization and scale, the area of mechanized planting has gradually expanded. The loss of soybean pods has become on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/63C07K14/415A01H5/00
Inventor 黄方喻德跃迟英俊王慧阚贵珍
Owner NANJING AGRICULTURAL UNIVERSITY
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