Soybean GmMADS2 Gene and Its Application

A technology for soybean and encoding genes, which can be applied in the fields of application, genetic engineering, plant genetic improvement, etc., and can solve the problems of easily cracking soybean pods

Inactive Publication Date: 2014-10-15
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an important leguminous plant, soybean has always occupied a special and important position in the nutrition of humans and animals with its high protein and high oil content, but soybean pods are easy to crack in the later harvest process

Method used

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  • Soybean GmMADS2 Gene and Its Application
  • Soybean GmMADS2 Gene and Its Application
  • Soybean GmMADS2 Gene and Its Application

Examples

Experimental program
Comparison scheme
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specific Embodiment approach （

[0027] Specific implementation methods (the results are explained below in conjunction with the figures)

[0028] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0029] The methods used in the following examples are conventional methods unless otherwise specified.

Embodiment 1

[0030] Embodiment 1, soybean SHP Gene GmMADS2 cDNA cloning and identification

[0031] soybeans ( Glycine max (L.) Merr.) variety Jackson was provided by the germplasm resource bank of the National Soybean Improvement Center of Nanjing Agricultural University. It was sown in the net room of Nanjing Agricultural University in early June and grown under natural conditions. We took the roots and leaves of soybean at the six compound leaf stage, the mixture of flowers and flower buds at the full flowering stage (R1) of soybean, and the pods of soybean at the initial pod stage (R3). The high sequence Gma.11881.1.A1_at in roots and leaves was also verified by the results of real-time quantitative RT-PCR. Further through the BLAST program, we used Gma.11881.1.A1_at as the probe sequence to search the soybean EST database, designed primers according to the results of sequence splicing, and used the cDNA of the mixture of soybean flowers and flower buds as a template to clone and...

Embodiment 2

[0035] Embodiment 2, soybean GmMADS2 Gene expression characteristics in different tissues

[0036] The soybean variety Jackson was sown in the net room of Nanjing Agricultural University in early June and grown under natural conditions. When the third compound leaf unfolds, get the roots, stems, leaves, and stem tips; when in full bloom, get the mixture of flowers and flower buds, the pods and pods of the soybean initial pod stage (R3); after collecting the fully bloomed mature flowers, Four round organs: calyx, petals, stamens and pistils were rapidly isolated under sterile conditions; seed coats, cotyledons and embryos of 20 DAF seeds were isolated under sterile conditions. After collection, the materials were quick-frozen in liquid nitrogen and stored at -80°C for later use.

[0037] The extraction of total RNA was the same as in Example 1. Constitutively expressed in soybean ActinThe gene (GenBank accession No.V00450) was used as an internal reference, and total RNA ...

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Abstract

The invention belongs to the field of plant genetic engineering and relates to soybean GmMADS2 gene and its application. The present invention clones a gene GmMADS2 that plays an important regulatory role in controlling pod cracking and flower organ development from soybean, and its sequence is SEQ ID NO.1. The mRNA expression analysis of this gene showed that it may be involved in the development of pistil, pod and seed coat. Further changes in the reproductive organs of Arabidopsis overexpressing GmMADS2 showed that GmMADS2 played an important regulatory role in controlling pod dehiscence and petal development. The invention discloses a genetic engineering improvement method for transforming plant reproductive organs with the gene. This method has a certain effect on the cultivation of plant varieties with altered reproductive organs, especially soybean varieties. It can improve the efficiency of seed production for crops by directional modification of the flower organs and pod morphology of crops, and then contribute to improving the yield of crops, especially soybeans. Serve.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering and relates to soybeans GmMADS2 Genes and their applications. Background technique [0002] SHP The gene belongs to the ovule characteristic gene and has the function of the D gene, which plays an important role in the determination of ovule characteristics, carpel development and fruit ripening and cracking. When the fruit is ripe, it will crack and release the seeds. Timely cracking is beneficial to the collection of seeds, and premature cracking is not conducive to the harvest of agricultural products. As an important leguminous plant, soybean has always occupied a special and important position in the nutrition of humans and animals with its high protein and high oil content, but soybean pods are easy to crack in the later harvesting process. Nowadays, with the continuous development of soybean industrialization and scale, and the gradual expansion of mechanized planting area, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/63C07K14/415A01H5/00
Inventor 黄方喻德跃迟英俊王慧阚贵珍
Owner NANJING AGRICULTURAL UNIVERSITY
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