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Human immunodeficiency virus type I Tat protein mutant 1-37-site sequence and application thereof

A technology for human immunodeficiency and protein mutants, which can be used in applications, viral peptides, antiviral agents, etc., and can solve problems such as low antibody titers and poor cross-reactivity

Inactive Publication Date: 2013-06-05
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sequence and structural characteristics of the above-mentioned HIV-1 Tat molecule itself make natural Tat a candidate antigen for HIV vaccines, which still has the disadvantages of inducing immune protective antibody titers and poor cross-reactivity.

Method used

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  • Human immunodeficiency virus type I Tat protein mutant 1-37-site sequence and application thereof
  • Human immunodeficiency virus type I Tat protein mutant 1-37-site sequence and application thereof
  • Human immunodeficiency virus type I Tat protein mutant 1-37-site sequence and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Through the construction of recombinant plasmids, prokaryotic expression and immunogenicity analysis of fusion proteins, three truncated Tat protein mutant sequences that can specifically react with HIV-1 full-length Tat proteins were obtained

[0044] 1. In vitro synthesis of the target gene

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Abstract

The invention relates to the technical field of biomedical engineering. HIV-1 type trans-activator of transcription (Tat) is an important regulatory protein produced at HIV-1 infection early stage. The invention aims at screening to obtain an HIV-1 type Tat protein mutant sequence with relatively stable molecular conformation and can start relatively strong immune response. The HIV-1 Tat protein mutant amino acid sequence is represented by SEQ ID NO: 2. Anti-rabbit serums of the Tat mutant protein are all subjected to specific reactions with HIV-1 full-length Tat protein, and the reactions are stronger. Therefore, the protein mutant can be used in preparing a component of an anti-HIV-infection peptide medicine, or as a candidate antigen in preparing HIV Tat vaccine.

Description

[0001] This application is a divisional application of the invention patent application submitted on March 25, 2011 with the patent application number 201110074185.7 and the invention title "Human Immunodeficiency Virus Type I Tat Protein Mutant Sequence and Its Application". technical field [0002] The invention relates to the technical field of biomedical engineering, in particular to human immunodeficiency virus type I Tat protein mutant sequence, and its application in the preparation of HIV immunogen, its application in the preparation of preventive and therapeutic HIV vaccines, and its application in the preparation of HIV detection application in the kit. Background technique [0003] Human immunodeficiency virus (Human immunodeficiency virus, HIV) is mainly transmitted through blood, sexual contact, and vertical channels from mother to child, and can cause acquired immunodeficiency syndrome (AIDS), that is, AIDS. According to the new statistics of UNAIDS at the end ...

Claims

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Application Information

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IPC IPC(8): C07K14/16C12N15/49C12Q1/70C12Q1/68A61K39/21A61K48/00A61P31/18G01N33/68
Inventor 曹洁张华群潘卫陈秋莉王锦红廖文婷祁培培杨界刘超何婷
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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