Neural stem cells

A neural stem cell, stem cell technology, applied in the field of neural stem cells

Active Publication Date: 2013-06-12
THE UNIV OF EDINBURGH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The culture methods of neural stem cells known in the art are (for reasons

Method used

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Embodiment 1-1

[0199] Isolation and culture of large populations of NS cells

[0200] ES cell differentiation protocols have been established in recent years to efficiently and consistently generate 50-70% Sox1-positive neural progenitor cells in adherent monolayer cultures. To isolate, expand and characterize neural precursor cells (derived from mice) in these cultures, a previously generated cell line (46C cells) containing a GFP-IRES-puromycin reporter cassette targeting the Sox1 locus was employed (Ying et al., 2003b). Puromycin was added to the differentiation culture after 7 days, and within 3 days, more than 95% of the remaining cells were GFP+ neural precursor cells. At this point, cells were re-plated in N2B27 medium supplemented with EGF / FGF-2 (without puromycin). These cells grow rapidly and assume a uniform morphology within several generations.

[0201] These 46C neural progenitor cells (46C-NP) maintained the characteristic bipolar morphology in continuous culture for more t...

Embodiment 1-2

[0203] Culture of Cell Lines

[0204] To isolate clonal cell lines, single cells from a large culture of passage 5 46C-NP cells were seeded in individual wells of a microplate. Of the 95 individual cells, 15 grew into colonies, and among these cells, 4 cell lines maintained continuous growth for more than 10 passages. One cell line (NP5) displayed characteristic bipolar cells with a homogeneous morphology as observed in the bulk population. This NP5 cell line can be maintained in culture indefinitely (NP541 passage; more than 5 months).

Embodiment 1-3

[0206] Characterization of NS cells

[0207] To characterize large populations and clonal cell lines, a panel of markers is assayed by immunocytochemistry. As expected, each cell line was found to be positive for the neural precursor cell markers stem protein or vimentin. Importantly, these cells were also positive for the radial glial cell-specific markers RC2, 3CB2, and astrocytic-specific glutamate transporter (GLAST). Less than 1% of cells were positive for GFAP or βIII-tubulin, suggesting that astrocytes or neurons rarely differentiated simultaneously during passage of these cells. Unlike primate / human cells, rodent radial glia are negative for GFAP. Furthermore, these cells were immunoreactive to the antibody SSEA-1 (previously used to enrich adult animal neural stem cells) recognizing the LewisX antigen.

[0208] RT-PCR was performed with a panel of markers to confirm their identity as radial glia. All four clonal cell lines, as well as a large population, were nega...

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Abstract

A homogenous, symmetrically dividing population of adherent neural stem cells is obtained from ES cells or foetal or adult brain isolates, using an activator of a signalling pathway downstream of a receptor of the EGF receptor family, optionally in combination with an activator of a signalling pathway downstream of an FGF receptor. The neural stem cell population is highly pure and retains the ability to differentiate into neurons after in excess of 100 passages.

Description

[0001] This application is a divisional application of the international application PCT / GB2005 / 002289, the international application date is June 9, 2005, the Chinese national phase application number 200580025096.6, and the invention patent application named "Neural Stem Cell". technical field [0002] The present invention relates to neural stem cells and culture conditions and methods for cultivating neural stem cells (NS cells or NSCs) to promote the symmetrical division and self-renewal of stem cells. Compositions, cell populations, cell lines, and individual neural stem cells are also provided. Background technique [0003] Although neural stem cells have been said to be isolated from a variety of sources in vitro, it has so far not been possible to expand cells in large-scale culture in a symmetrically dividing, undifferentiated state. Long-term expansion of cells in this state is highly desirable from both an experimental and a therapeutic point of view. The result...

Claims

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Application Information

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IPC IPC(8): C12N5/0797A61K35/30A61P25/28A61P25/00C12R1/91A61K35/12C12N5/02
CPCA61K35/12C12N2501/11C12N2501/115C12N2506/02C12N2533/52C12N5/0623A61P25/00A61P25/02A61P25/14A61P25/16A61P25/28A61P9/10
Inventor L·孔蒂S·M·波拉德A·G·史密斯
Owner THE UNIV OF EDINBURGH
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