Universal PCR detection kit for cat and dog parvovirus

A technology for detecting kits and parvovirus, which is applied in the diagnosis of animal infectious diseases and the field of general-purpose PCR kits. And other issues

Inactive Publication Date: 2013-06-19
WITHYOU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been reports on the PCR detection method for canine parvovirus, there is no report on the PCR detection kit for feline parvovirus, nor is there any report on a universal kit that can simultaneously detect cat and dog parvovirus

Method used

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  • Universal PCR detection kit for cat and dog parvovirus
  • Universal PCR detection kit for cat and dog parvovirus
  • Universal PCR detection kit for cat and dog parvovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take a sick cat suspected of being infected with parvovirus, scrape a little feces, and operate according to the procedure of the kit. The results are shown in the attached figure 1 shown. Based on the results, it was judged that the cat was only infected with parvovirus. The test paper test result was also positive, confirming that the PCR test result was correct.

Embodiment 2

[0027] Take a sick dog suspected of parvovirus infection, scrape a little nasal secretion, and operate according to the procedure of the kit. The results are shown in the attached figure 2 shown. Based on the results, it was judged that the dog was infected with parvovirus. The test paper test result was also positive, confirming that the PCR test result was correct.

Embodiment 3

[0030]

[0031]

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PUM

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Abstract

The invention discloses a universal PCR detection kit for rapid and high-efficient detection of cat and dog parvovirus. The method comprises designing a pair of primers in accordance with a conservation region of a vp2 gene of the cat and dog parvovirus, performing a PCR reaction by using excrement of detected animals as a template, comparing with negative and positive controls included in the kit, and determining whether the detected animals are infected with the parvovirus. The detection kit is simple, easy to use, high in sensitivity, and good in stability, and can be used for monitoring health conditions of the cat and the dog.

Description

technical field [0001] The invention belongs to the diagnosis technology of animal infectious diseases, and relates to a detection method for parvovirus in cats and dogs. [0002] The invention provides a universal PCR kit for detecting parvovirus in cats and dogs. The content of the present invention relates to a pair of PCR primers that can simultaneously detect parvovirus in cats and dogs, and a set of PCR detection consisting of PCR reaction mix (including primers), positive control, negative control, and bromophenol blue loading buffer Kits and corresponding detection procedures. Background technique [0003] Feline parvovirus (felinepanleukopenia virus, FPV), also known as feline panleukopenia virus, feline infectious enteritis virus or feline distemper virus, is one of the main pathogens that harm cats; while canine parvovirus (CPV) is The main pathogen causing acute hemorrhagic gastroenteritis in dogs and myocarditis in puppies. The clinical symptoms of infected a...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 朱威贺雄雷潘岷溟薛虎平郭蕾王松鹤
Owner WITHYOU BIOTECH
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