Full-automatic accurate-timing quantitative quick-sampling equipment capable of in-place inactivation

Abstract

The invention relates to full automatic accurate-timing quantitative quick-sampling equipment capable of in-place inactivation. The equipment comprises a mechanical transmission device, a sampling device and a control device, wherein the control device is used for controlling the sampling device and the mechanical transmission device in a linkage manner; the mechanical transmission device comprises an electric motor, a transmission shaft, a sampling turntable and a liquid storage tank; the electric motor is used for driving the rotation of the sampling turntable through the transmission shaft; a plurality of positioning sampling holes for placing sampling pipes are formed in the sampling turntable; the liquid storage tank is arranged below the sampling turntable; and the control device is used for firstly positioning the positions of the positioning sampling holes in the sampling turntable and then controlling the sampling device to quantitatively sample during sampling. Compared with the prior art, the device has the advantages that the whole process of quick, quantitative, continuous and timing sampling and quenching is completed in a few seconds, full automatization can be realized during the whole process, and a single sample is once sampled within one second, so that the true situation of cells can be really reflected by cytozoic metabolites after quenching, and the cytozoic metabolites are effectively prevented from being transformed and degraded.

Description

technical field [0001] The invention relates to a rapid sampling device for cell physiological metabolism research, in particular to a fully automatic rapid sampling device capable of precise timing, quantification and on-site inactivation. Background technique [0002] In order to study the physiological metabolic characteristics of various cells such as microorganisms, plants and animals under different physiological states (such as metabolomics and metabolomics), it is necessary to remove the cells from the culture medium. During the sampling process, because the enzyme-catalyzed reaction in the cell is very fast (especially the conversion of intermediate metabolites in the central carbon metabolic pathway is generally at the second or sub-second level), the target metabolites may undergo structural changes, concentration changes, or even Since it is completely converted into other substances, it is difficult to carry out accurate qualitative and quantitative analysis of ...

AI Technical Summary

Problems solved by technology

This method can accurately determine the sampling time after the pulse is added, but high temperature will degrade some substances, and there are also limitations in application

Some literatures use solenoid valves to take samples into the cold quenching liquid, and are equipped with a sampling turntable, which can realize continuous sampling. However, the valve system and external power sampling time still have limitations that are not fast enough, and it is difficult to achieve quantitative sampling.

Images