Method for preparing compound enzyme and probiotic preparation for feed through biotransformation of bagasse and sunflower cake

A probiotic preparation and biotransformation technology, applied in animal feed, animal feed, applications, etc., can solve problems such as difficult digestion of monogastric animals, and achieve the effects of low-cost, high-efficiency biotransformation, and strong enzyme production capacity

Active Publication Date: 2014-10-08
金华市金帆饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of bagasse, 5kg of sunflower cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and acclimatize at 35°C After cultivating for 48 hours, a leavening agent is formed;

[0028] 2) Mix 20kg of starter in step 1) with 600kg of bagasse, 300kg of sunflower cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 66.22IU / g; total cellulase activity reaches 4.98IU / g; beta-glucosidase activity reaches 4.98IU / g; reached 9.34IU / g; endoglucanase activity reached 45.36IU / g; xylanase act...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of bagasse, 5kg of sunflower cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimatize at 35°C After cultivating for 48 hours, a leavening agent is formed;

[0032] 2) Mix 20kg of starter in step 1) with 600kg of bagasse, 300kg of sunflower cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 65.73IU / g; total cellulase activity reaches 4.87IU / g; beta-glucosidase activity reaches 4.87IU / g; reached 9.28IU / g; endoglucanase activity reached 45.29IU / g; xylanase act...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of bagasse, 5kg of sunflower cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimatize at 40°C After culturing for 36 hours, a starter culture was formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of bagasse, 300kg of sunflower cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and ferment at 35°C for 5 Day, get the compound enzyme and probiotic preparation for feed.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 62.36IU / g; total cellulase activity reaches 4.43IU / g; beta-glucosidase activity reaches 62.36IU / g; reached 8.85IU / g; endoglucanase activity reached 46.55IU / g; xylanase act...

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PUM

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Abstract

The invention discloses a method for preparing a complex enzyme-probiotic preparation for feeds through biotransformation of bagasse and sunflower cakes. The method comprises the steps of fully mixing Puer ripe tea subjected to pile fermentation, lentinus edobes sticks, agrocybe cylindracea sticks, first bagasse, first sunflower cakes, first wheat bran and first apple pomace with first water so as to form a leaven after acclimation and culture; and fully mixing the leaven, second bagasse, second sunflower cakes, second wheat bran and second apple pomace with second water so as to obtain the complex enzyme-probiotic preparation for feeds after fermentation. The complex enzyme-probiotic preparation for feeds, with better performance indexes, is prepared by fermenting bagasse and sunflower cakes with food-grade safe floras with stable micro-ecology, thus achieving low-cost and efficient biotransformation of bagasse and sunflower cakes. Meanwhile, the method has the advantages of easiness in microorganism growth, high enzyme activity and extensive fermentation processes, does not need strict aseptic conditions, and easily achieves large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting bagasse and sunflower cake by adopting micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
CPCY02P60/87
Inventor 阮晖项云徐志钱春芳陈功诸文颖纪晓燚吴渊杜姗姗杨璐
Owner 金华市金帆饲料有限公司
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