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Application of BDNA for detecting PCA3 in urine

A urine and specimen technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as false negatives, achieve high positive detection rate, easy popularization, good stability and repeatability Effect

Inactive Publication Date: 2013-12-11
KODIA XINXIANG BIO TECH
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AI Technical Summary

Problems solved by technology

Because the amplification of this technology is amplified with primers, the region that needs to be amplified is complete, such as RNA fragmentation and degradation or mutation in the specimen cannot be combined with the primers, resulting in false negatives

Method used

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  • Application of BDNA for detecting PCA3 in urine
  • Application of BDNA for detecting PCA3 in urine
  • Application of BDNA for detecting PCA3 in urine

Examples

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Embodiment Construction

[0030] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0031] A kind of application of BDNA detection PCA3 in urine, the operation steps adopted are:

[0032] (1) PCA3 specimen collection

[0033] Urine PCA3 test uses the urine sample after rectal prostate massage:

[0034] 1) Drinking 500ml of water before the examination will help to collect urine samples;

[0035] 2) Collect urine immediately after prostate digital examination;

[0036] 3) At least 20-30ml of urine should be collected in a labeled cup, which must be the first excreted urine sample, and the urine cup for storing urine should not contain any preservatives;

[0037] 4) If the specimen is stored for a long t...

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PUM

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Abstract

The invention provides an application of BDNA for detecting PCA3 in urine. Operation steps comprise (1) collecting PCA3 specimens; (2) studying the collection of the PCA3 specimens; (3) detecting with a BDNA technology. By utilizing the bDNA technology being good at capture of small fragment genes, efficiently specific combination of uniquely designed target probes and PCA3 and an improved signal amplifying system, both sensitivity and specificity are guaranteed. The technology prevents the problems of false positive and false negative of conventional PCR, is simple in operations, and can be operated by a person with basic skills of molecular biology experiments. Meanwhile, the technology has high detected result sensitivity, high positive detectable rate and good stability and repeatability, is convenient for operations, is time-saving and can be popularized easily.

Description

technical field [0001] The invention relates to the field of prostate cancer detection, in particular to an application of BDNA to detect PCA3 in urine. Background technique [0002] At present, early diagnosis methods of prostate cancer and PCA3 dominance Prostate cancer (PCa) is the most common malignant tumor in men in western countries, ranking second in the cause of death. With the aging population, westernization of diet structure and improvement of diagnostic techniques in my country, the incidence and diagnosis rate of PCa are increasing year by year. [0003] The most commonly used marker for the diagnosis of prostate cancer is PSA, but PSA is a prostate tissue-specific antigen, not a PCa-specific antigen, so benign prostatic hyperplasia, prostatitis, acute urinary retention, various surgical operations and examinations of the prostate can cause serum High PSA, poor disease specificity. Only 1 out of every 4 elderly men with serum PSA>4ng / ml in the United State...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 张鹭鹭张爱国刘桐宇
Owner KODIA XINXIANG BIO TECH
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