Application of rifampicin in preparation of tobacco whitefly insecticides
A technology of insecticide and whitefly, which is applied in the application field of rifampicin in the preparation of whitefly insecticide, can solve the problems of little control effect, cumbersome operation process and difficult implementation, so as to reduce hatching High rate, good control effect and easy operation
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Embodiment 1
[0021] Example 1 Observation of the control effect of rifampicin on the population level of Bemisia tabaci
[0022] 1 Rifampicin to treat Bemisia tabaci
[0023] (1) Weigh 0.01g of rifampicin and 25g of sucrose, add 100mL of water to make 100μg / mL rifampicin sucrose mother solution, and dilute to 60μg / mL, 50μg / mL, 40μg / mL, 30μg / mL, 25μg / mL , 20μg / mL, 15μg / mL, 10μg / mL, 5μg / mL, 2.5μg / mL, 1μg / mL, 0.5μg / mL, 0.2μg / mL, 0.1μg / mL together with the mother solution, a total of 15 concentrations are ready for use; Set 25% sucrose solution as a control.
[0024] (2) For a concentration of rifampicin solution, take 600 heads of Q and B whiteflies that have first emerged, respectively, and introduce 100 heads / tube into the double-pass glass tubes prepared in advance. Each glass tube The upper part of the tube is covered with two layers of Parafilm membranes; the two layers of Parafilm membranes of the three glass tubes are sandwiched with the prepared rifampicin solution, and the two laye...
Embodiment 2F1
[0035] Embodiment 2 F1 generation adult fecundity assay
[0036] In order to verify the authenticity of the results obtained in Example 1, the Q whitefly was further treated with a rifampicin solution of 0.5-100 μg / mL, and the B whitefly was treated with a rifampicin solution of 1-100 μg / mL. Fertility is measured, the steps include:
[0037] (1) In the afternoon before the measurement, 150-180 pseudo pupae from the treatment group and the control group were cut out respectively, and each pseudo pupae was put into a finger-shaped glass tube until it emerged;
[0038] (2) On the morning of the measurement day, identify the sex of the newly emerged F1 adults in each finger-shaped glass tube;
[0039] (3) For Q bemisia tabaci, 10♀×10♂ was used as an egg-laying group, treatment group and control group were respectively set up in 8 groups, and eggs were laid for 2 days; for B bemisia tabaci, 3♀×3♂ was used as an The egg-laying group, control and treatment set up 12 groups respecti...
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