Application of rifampicin in preparation of tobacco whitefly insecticides

A technology of insecticide and whitefly, which is applied in the application field of rifampicin in the preparation of whitefly insecticide, can solve the problems of little control effect, cumbersome operation process and difficult implementation, so as to reduce hatching High rate, good control effect and easy operation

Inactive Publication Date: 2014-01-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the possibility of completely killing insects is very low, and it is inevitable that there will be omissions when selecting insect-free seedlings, which makes it difficult to implement
[0007] (2) Physical control: Bemisia tabaci has a strong tendency to yellow, especially orange-yellow, and yellow boards coated with viscous oil can be set up to trap and kill adults; Egg control has little effect, and the operation process is relatively cumbersome
However, due to resource and cost constraints, natural enemy insects have not yet been able to be used in large-scale control of Bemisia tabaci in the field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Observation of the control effect of rifampicin on the population level of Bemisia tabaci

[0022] 1 Rifampicin to treat Bemisia tabaci

[0023] (1) Weigh 0.01g of rifampicin and 25g of sucrose, add 100mL of water to make 100μg / mL rifampicin sucrose mother solution, and dilute to 60μg / mL, 50μg / mL, 40μg / mL, 30μg / mL, 25μg / mL , 20μg / mL, 15μg / mL, 10μg / mL, 5μg / mL, 2.5μg / mL, 1μg / mL, 0.5μg / mL, 0.2μg / mL, 0.1μg / mL together with the mother solution, a total of 15 concentrations are ready for use; Set 25% sucrose solution as a control.

[0024] (2) For a concentration of rifampicin solution, take 600 heads of Q and B whiteflies that have first emerged, respectively, and introduce 100 heads / tube into the double-pass glass tubes prepared in advance. Each glass tube The upper part of the tube is covered with two layers of Parafilm membranes; the two layers of Parafilm membranes of the three glass tubes are sandwiched with the prepared rifampicin solution, and the two laye...

Embodiment 2F1

[0035] Embodiment 2 F1 generation adult fecundity assay

[0036] In order to verify the authenticity of the results obtained in Example 1, the Q whitefly was further treated with a rifampicin solution of 0.5-100 μg / mL, and the B whitefly was treated with a rifampicin solution of 1-100 μg / mL. Fertility is measured, the steps include:

[0037] (1) In the afternoon before the measurement, 150-180 pseudo pupae from the treatment group and the control group were cut out respectively, and each pseudo pupae was put into a finger-shaped glass tube until it emerged;

[0038] (2) On the morning of the measurement day, identify the sex of the newly emerged F1 adults in each finger-shaped glass tube;

[0039] (3) For Q bemisia tabaci, 10♀×10♂ was used as an egg-laying group, treatment group and control group were respectively set up in 8 groups, and eggs were laid for 2 days; for B bemisia tabaci, 3♀×3♂ was used as an The egg-laying group, control and treatment set up 12 groups respecti...

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PUM

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Abstract

The invention discloses an application of rifampicin in preparation of tobacco whitefly insecticides. The invention firstly proposes that the rifampicin can be taken as an agricultural antibiotic for preparing the tobacco whitefly insecticides and controlling hemiptera piercing sucking insects such as whiteflies. Compared with the prior art, the application disclosed by the invention can utilize the rifampicin to prepare the tobacco whitefly insecticides, so that the egg laying amount of tobacco whitefly F1-generation females can be greatly reduced, the incubation of insect eggs can be inhibited and further the population growth of tobacco whitefly can be effectively controlled.

Description

technical field [0001] The invention relates to an application of rifampicin, in particular to the application of rifampicin in the preparation of whitefly insecticide. Background technique [0002] Bemisia tabaci (Gennadius) is a polyphagous piercing-sucking pest that harms many crops such as tomatoes, cucumbers, peppers, and cotton, and is distributed all over the world. Adults and nymphs of Bemisia tabaci not only suck plant juice to cause direct damage, but also spread a variety of plant virus diseases to cause greater indirect damage. [0003] Bemisia tabaci originally originated in tropical and subtropical regions. Since the 1980s, with the worldwide trade, Bemisia tabaci has spread rapidly with the help of seedlings of flowers and other economic crops, and has spread widely around the world and caused outbreaks. It has become an important pest in the agricultural production of countries such as the United States, China, India, Pakistan, Sudan and Israel, among which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P7/04
Inventor 王晓伟张昌容单红伟刘树生
Owner ZHEJIANG UNIV
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