A kind of breeding method of bolting-resistant radish inbred line
A bolting-resistant, inbred line technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve the problems of long breeding cycle, difficult breeding of inbred lines, easy loss of breeding materials, etc. The effect of stable bolting resistance and excellent commercial properties
Active Publication Date: 2014-10-22
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology
[0005] In view of the above-mentioned prior art, the purpose of the present invention is to establish a set of methods for accelerating the selection of bolting-resistant radish inbred lines, so as to solve the problems of difficult breeding of inbred lines, easy loss of breeding materials, and long breeding cycle in the spring radish breeding process. problem, can greatly speed up the breeding of spring radishes
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Embodiment 1
[0045] Example 1 Breeding of bolting-resistant radish inbred lines
[0046] Proceed as follows:
[0047] (1) On November 15th, put filter paper in the plate, add water to make it moist, put radish seeds on it, and accelerate germination at 25°C for 16-24 hours. wet state. After the germination is completed, about 90% of the seeds germinate and can be vernalized at 2-4°C for 15-20 days. In order to prevent the water from evaporating too fast, seal it with a parafilm.
[0048] (2) Vernalization is completed around December 1, sow the radish buds in seedling pots, and put them in a solar greenhouse or artificial temperature-controlled cultivation room for centralized management. Ensure that the daytime temperature of the solar greenhouse is 15-25°C, and the night temperature is above 5°C. The temperature around New Year's Day is relatively low. In order to increase the temperature and accelerate its growth, add a layer of plastic film to raise the temperature.
[0049] (3) Aro...
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The invention discloses a breeding method for a bolting resistant radish selfing line, aims to solve the problems of difficult seed reproduction of selfing line during the breeding process of spring radish, easy loss of breeding materials, and long breeding period, and is capable of greatly promoting the breeding speed of spring radish. The method comprises the following steps: (1) accelerating germination, carrying out a vernalization treatment; (2) culturing, (3) transplanting the seedlings into planting pits in a green house; (4) carrying out bolting property screening, removing the strains which have bolted already; (5) carrying out a selfing and pollinating treatment for the left bolting resistant strains; (6) collecting part of pods from the strains, stripping the pod hulls and taking out the embryos, culturing the embryos, pollinating the left pods, saving the seeds; (7) transplanting the seedlings which are obtained through the embryo culture to fields, covering film over the plants after the field planting; (8) harvesting the seeds; (9) carrying out statistic on the bolting rate of the radish planted in the fields and the commercial properties of the fleshy roots, selecting the strain line, whose bolting rate is less than 10%, fleshy root can be harvested within 65 days, and output is 3500 kg or more, and the selected strain line is the bolting resistant radish selfing line.
Description
technical field [0001] The invention relates to a method for breeding a bolting-resistant radish inbred line, and belongs to the technical field of breeding of new agricultural varieties. Background technique [0002] Radish (Raphanus sativus L.) belongs to the genus Radish of Cruciferae. The edible part is a hypertrophic fleshy root. The mustard oil and dietary fiber contained in the root can promote gastrointestinal motility, and the various trace elements contained in it can induce the human body to interfere It is of great significance to anti-cancer and anti-cancer. Eating radish often can also reduce blood fat, soften blood vessels, and stabilize blood pressure. Therefore, radish has always had high edible and medical value. [0003] At present, with the development of production and the improvement of living standards, people's requirements for the quality and supply period of radish products are also increasing, and more types of varieties are needed to meet the need...
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IPC IPC(8): A01H1/04A01H4/00
Inventor王淑芬徐文玲刘贤娴付卫民赵智中李巧云张志刚刘栓桃
OwnerVEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI