The invention relates to a ganoderma leucocontextum strain L4495 as well as a cultivation method and application thereof, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center in China on December 27, 2021, and the preservation number is GDMCC No: 62104. According to the method, the strain is prepared through a liquid fermentation culture method, the obtained liquid strain is inoculated into a cultivation bag, and the strain is planted under the economic forest after spawn running is completed, so that compared with facility cultivation, planting under the economic forest is less in investment, simple in management, high in quality and good in benefit, and the polysaccharide content of the cultivated white-meat ganoderma lucidum L4495 is 1.58%, and the triterpene content is 3.26%. According to the method, a Ganoderma leucoderma wild strain resource suitable for under-forest cultivation is obtained through domestication, liquid submerged fermentation culture is carried out, and a foundation is laid for remote protection and further development and utilization of germplasm resources of Ganoderma lucidum. In addition, sporocarp or fermentation liquor of the ganoderma leukemia strain L4495 can be used for preparing anti-tumor drugs.