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Lucid ganoderma strain L4495 as well as cultivation method and application thereof

A technology of white-fleshed Ganoderma lucidum and its cultivation method, which is applied in the field of white-fleshed Ganoderma lucidum strain L4495 and its cultivation method and application, and can solve problems such as no research reports on strains

Pending Publication Date: 2022-07-22
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The Ganoderma lucidum strain of this application is a new strain collected and isolated on the mountain of Laomuba Village, Jiadian Town, Wuding County, Chuxiong Prefecture, Yunnan Province. After research, there is no research report on this strain

Method used

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  • Lucid ganoderma strain L4495 as well as cultivation method and application thereof
  • Lucid ganoderma strain L4495 as well as cultivation method and application thereof
  • Lucid ganoderma strain L4495 as well as cultivation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0056] The cultivation method of white-fleshed Ganoderma lucidum strain L4495, comprising the following steps:

[0057] Step (1), by taking the white-fleshed Ganoderma lucidum strain L4495 tissue under aseptic conditions, inoculating it on the PDA medium, sealing and placing it in an incubator at 24°C for constant temperature cultivation for 10d;

[0058] Step (2), inoculate the strains cultivated in step (1) in a liquid culture medium, and place in a shaker for 12 days at 27° C. and 150 r / min;

[0059] Among them, the liquid medium is: soluble starch 6g, soybean powder 0.5g, potassium dihydrogen phosphate 0.3g, MgSO 4 ·7H 2 O0.5g, VB 2 0.1g, water 200mL; pH=7;

[0060] Step (3), inoculate the cultured liquid bacterial strain in step (2) into the cultivation bag, and place it in a 24° C. cultivation room for dark cultivation until the mycelium is covered with the cultivation bag;

[0061] The cultivation substrate in the cultivation bag includes the following components i...

Embodiment 2

[0064] The cultivation method of white-fleshed Ganoderma lucidum strain L4495, comprising the following steps:

[0065] Step (1), under aseptic conditions, by taking the white-fleshed Ganoderma lucidum strain L4495 tissue, inoculating it on the PDA medium, and sealing and placing it in an incubator at 24 ° C for constant temperature cultivation for 9d;

[0066] Step (2), inoculate the strains cultivated in step (1) in a liquid medium, and place in a shaker for 11 days at 26° C. and 140 r / min;

[0067] Among them, the liquid medium is: soluble starch 5.9g, soybean powder 0.48g, potassium dihydrogen phosphate 0.29g, MgSO 4 ·7H 2 O 0.48g, VB 2 0.09g, water 200mL; pH=6.9;

[0068] Step (3), inoculate the cultured liquid bacterial strain in step (2) into a cultivation bag, and place it in a 23° C. cultivation room for dark cultivation until the mycelium is covered with the cultivation bag;

[0069] The cultivation substrate in the cultivation bag includes the following compone...

Embodiment 3

[0077] The cultivation method of white-fleshed Ganoderma lucidum strain L4495, comprising the following steps:

[0078] Step (1), under aseptic conditions, by taking the white-fleshed Ganoderma lucidum strain L4495 tissue, inoculating it on the PDA medium, sealing and placing it in an incubator at 24 ° C for constant temperature cultivation for 11 d;

[0079] Step (2), inoculate the strains cultivated in step (1) in a liquid medium, and place in a shaker for 13 days at 28° C. and 160 r / min;

[0080] Among them, the liquid medium is: soluble starch 6.1g, soybean powder 0.51g, potassium dihydrogen phosphate 0.31g, MgSO 4 ·7H 2 O 0.52g, VB 2 0.11g, water 200mL; pH=7.1;

[0081] Step (3), inoculate the cultured liquid bacterial strain in step (2) into a cultivation bag, and place it in a 25° C. cultivation room for dark cultivation until the mycelium is covered with the cultivation bag;

[0082] The cultivation substrate in the cultivation bag includes the following component...

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Abstract

The invention relates to a ganoderma leucocontextum strain L4495 as well as a cultivation method and application thereof, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center in China on December 27, 2021, and the preservation number is GDMCC No: 62104. According to the method, the strain is prepared through a liquid fermentation culture method, the obtained liquid strain is inoculated into a cultivation bag, and the strain is planted under the economic forest after spawn running is completed, so that compared with facility cultivation, planting under the economic forest is less in investment, simple in management, high in quality and good in benefit, and the polysaccharide content of the cultivated white-meat ganoderma lucidum L4495 is 1.58%, and the triterpene content is 3.26%. According to the method, a Ganoderma leucoderma wild strain resource suitable for under-forest cultivation is obtained through domestication, liquid submerged fermentation culture is carried out, and a foundation is laid for remote protection and further development and utilization of germplasm resources of Ganoderma lucidum. In addition, sporocarp or fermentation liquor of the ganoderma leukemia strain L4495 can be used for preparing anti-tumor drugs.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a white-fleshed Ganoderma lucidum strain L4495 and a cultivation method and application. Background technique [0002] Ganoderma spp. belongs to Basidiomycota, Polyporals, Ganodermataceae, Ganoderma, and is known as the king of herbs. Fruiting bodies grow solitary and group saprophytic on dead standing trees, fallen trees, stumps or living trees in summer and autumn. It is a precious traditional Chinese medicinal material in my country, which has a medicinal history of more than 2,000 years in China. Li Shizhen's "Compendium of Materia Medica" recorded in the Ming Dynasty Ganoderma lucidum: its taste is bitter and flat, non-toxic, invigorating the middle and invigorating qi, increasing wisdom, good color, long-term eating, lightening the body and not getting old, and prolonging life. Modern medical research has proved that active ingredients such as triterpenes...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20A01G18/50
CPCA01G18/40A01G18/20A01G18/50
Inventor 何俊李树红李娥贤叶洋罗红梅方晓军
Owner 云南省农业科学院生物技术与种质资源研究所
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