Rejuvenation method of tremella aurantialba sporocarp strain
A golden ear fruiting body, golden ear kid technology, applied in the fields of botany equipment and methods, horticulture, agriculture, etc., can solve the problems of reduced yield, no harvest, carrying viruses and bacteria, etc., to avoid risks, restore biological traits, avoid The effect of production reduction or even extinction
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[0031] 1. Collection of golden ears
[0032] Select a healthy, well-proportioned, golden-yellow first crop of golden ears in the fruiting shed.
[0033] 2. Production of golden ear rejuvenation medium
[0034] Improved PDA medium: potato 200 g / L, glucose 10 g / L, peptone 3 g / L, potassium dihydrogen phosphate 3 g / L, citric acid 1.2 g / L, shell powder 1 g / L, agar 18 g / L L.
[0035] Prepare the culture medium according to the above formula, and sterilize it at 121°C for 20 minutes, pour it into a petri dish on the ultra-clean workbench, and cool it for later use.
[0036] 3. Process flow for the rejuvenation of fruiting body strains
[0037] 3.1 Coating and inoculation
[0038] In an ultra-clean workbench, mix 0.5 g of aseptic Auricularia aureus fruiting body with 5 ml of sterilized water, beat it into a slurry with a crusher, and spread it on the improved PDA medium.
[0039] 3.2 Cultivation
[0040] Cultivate in dark for 25-35 days in a 22°C incubator, and a plurality of sm...
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