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Rejuvenation method of tremella aurantialba sporocarp strain

A golden ear fruiting body, golden ear kid technology, applied in the fields of botany equipment and methods, horticulture, agriculture, etc., can solve the problems of reduced yield, no harvest, carrying viruses and bacteria, etc., to avoid risks, restore biological traits, avoid The effect of production reduction or even extinction

Active Publication Date: 2020-12-04
云南菌视界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although golden ear has been successfully cultivated for more than 30 years, the whole golden ear industry is still in its infancy (annual output is far less than the daily output of Flammulina velutipes), no matter whether it is using the step-by-step inoculation method or the mixed strain method, the fruiting bodies are all It is the source of provenance. With the expansion of the golden ear fruiting body, the number of reproductions increases, the production performance of the strains degrades rapidly, and the strains carry viruses and bacteria, etc., which reduces the success rate of cultivation, reduces the yield, and even fails to harvest.

Method used

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  • Rejuvenation method of tremella aurantialba sporocarp strain
  • Rejuvenation method of tremella aurantialba sporocarp strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] 1. Collection of golden ears

[0032] Select a healthy, well-proportioned, golden-yellow first crop of golden ears in the fruiting shed.

[0033] 2. Production of golden ear rejuvenation medium

[0034] Improved PDA medium: potato 200 g / L, glucose 10 g / L, peptone 3 g / L, potassium dihydrogen phosphate 3 g / L, citric acid 1.2 g / L, shell powder 1 g / L, agar 18 g / L L.

[0035] Prepare the culture medium according to the above formula, and sterilize it at 121°C for 20 minutes, pour it into a petri dish on the ultra-clean workbench, and cool it for later use.

[0036] 3. Process flow for the rejuvenation of fruiting body strains

[0037] 3.1 Coating and inoculation

[0038] In an ultra-clean workbench, mix 0.5 g of aseptic Auricularia aureus fruiting body with 5 ml of sterilized water, beat it into a slurry with a crusher, and spread it on the improved PDA medium.

[0039] 3.2 Cultivation

[0040] Cultivate in dark for 25-35 days in a 22°C incubator, and a plurality of sm...

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Abstract

The invention belongs to the technical field of edible fungus breeding, and particularly relates to a rejuvenation method of tremella aurantialba sporocarp strain. The rejuvenation method comprises the steps of pulping tremella aurantialba sporocarp once or multiple times and coating an improved PDA culture medium with slurry for culture. The method aims to rejuvenate, detoxify and remove infectious microbes for a tremella aurantialba sporocarp degenerated strain, the production capacity and disease resistance of the strain can be effectively recovered, and risks are avoided for farmer households.

Description

technical field [0001] The invention belongs to the technical field of edible fungus breeding, and in particular relates to a method for rejuvenating fungus fruiting bodies. Background technique [0002] Golden ear Naematelia aurantialba (Bandoni et Zang) Millanes&Wedin is a fungus of the genus Auriculariaceae, (the former scientific name of golden ear is Tremella aurantialba Bandoni et Zang, which belongs to the fungus of the genus Tremella family Tremella), is a famous edible fungus and For medicine and medicine, wild resources are mainly distributed in Lijiang, Shangri-La, Tibet and other places in Yunnan. The resources are limited, and the annual national picking amount is less than 10 tons (dry). In the 1980s, the Yunnan Kunming Edible Fungi Research Institute was the first to realize artificial basswood and bag material cultivation. [1-2] . [0003] The fruiting body of golden ear is a heterogeneous complex. In the fruiting body of golden ear, there are Bacillus cham...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/60
CPCA01G18/00A01G18/20A01G18/60
Inventor 李荣春李梦杰杨林雷
Owner 云南菌视界生物科技有限公司
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