Total DNA (deoxyribonucleic acid) extraction method and kit for synchronously removing humic acid and mycoprotein
A technology of bacterial protein and extraction method, which is applied in the research field of metagenomics in soil microorganisms and general microbiology, and achieves the effect of low cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0031] The present invention will be described in detail below with the best embodiment 。
[0032] The soil for the test was collected from the surface soil with oncomelania gathering points and no oncomelania points on grasslands in different areas of Poyang Lake District, Jiangxi Province (soil at the contact surface of oncomelania and 5cm below) 1, 2, 3, 4, 5, 8, 9, Soil samples No. 10 and No. 11.
[0033] The method for extracting total DNA is as follows:
[0034] 1) Take 0.4g soil sample and put it in a 2ml centrifuge tube, add 1ml humic acid complex solution [100 mmol / L sodium pyrophosphate (Na 4 P 2 o 7 ), 20 mmol / L EDTA -2Na and 150 mmol / L NaCl, pH9-10], shake and mix for 5 minutes to redissolve the humate in the soil, centrifuge at 12000r / min for 5 minutes, discard the supernatant to wash away the humic acid , then add 1ml of PBS buffer [140mmol / L NaCl, 2.7mmol / L KCl, 7.3mmol / L Na 2 HPO 4 and 1.5mmol / L KH 2 PO 4 , pH 7.0--7.4], stir and mix with a to...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com