9 results about "Isoprodian" patented technology

This invention discloses a purification method for Tirzepatide. The method includes: dissolving crude Tirzepatide peptide in ammonia or sodium bicarbonate solution, filtering, and purifying by reversed-phase chromatography to obtain a purified sample; eluting the purified sample using reversed-phase high-performance liquid chromatography (RP-HPLC) with a sodium or potassium buffer solution as mobile phase A3 and one or a mixture of acetonitrile, methanol, and isopropanol in any proportion as mobile phase B, preparing a sodium or potassium salt fraction; cooling the sodium or potassium salt fraction to -20 to 10°C and maintaining the temperature; then adding an organic solvent to the fraction, controlling the temperature at -20 to 10°C to precipitate the sample; after complete precipitation, solid-liquid separation is performed, the solid is collected, dissolved in water, filtered, and freeze-dried. This invention improves the concentration speed, ensures product purity, and strictly controls impurities.

This invention relates to the field of fecal genomics kit technology, and particularly to a safe, rapid, and high-throughput kit for extracting fecal genomes and its usage method. The kit includes a sample preservation solution, magnetic beads, a lysis buffer, a washing buffer 1, a washing buffer 2, and an elution buffer. The sample preservation solution is composed of guanidine isothiocyanate, sodium citrate, Triton X-100, Tween 20, propidium iodide, and purified water. The magnetic beads are hydroxyl magnetic beads. The lysis buffer is composed of guanidine hydrochloride, Tris base, Tris hydrochloric acid, Triton X-100, isopropanol, and purified water. Washing buffer 1 is composed of guanidine hydrochloride solution and isopropanol. Washing buffer 2 is composed of anhydrous ethanol and purified water. This kit can easily extract the genome from fecal samples, is simple to operate, safe and non-toxic, and can extract multiple samples at once, enabling rapid diagnosis and filling the gap in the current market for automated high-throughput kits for extracting genomes from fecal samples.

The invention discloses a preparation method of an antibacterial coating material based on synergistic interaction of antibiotics and metal ions. The preparation method comprises the following steps: (1) blending antibiotics, soluble salts of metal ions, an absorbable polymer and a mixed solvent to obtain a coating solution; the antibiotics are selected from rifampicin and minocycline hydrochloride; the metal ions are selected from Zn and / or Cu; the mixed solvent is selected from hexafluoroisopropanol and a methanol / aqueous solution, and the volume ratio of hexafluoroisopropanol in the mixed solvent is not less than 25%; and (2) uniformly spraying the coating solution on the surface of the degradable base material through ultrasonic spraying, and then carrying out drying treatment. The preparation method is easy and convenient to operate, the obtained coating is tightly combined with a degradable base material, and the antibacterial rate can still reach 90% or above after 50 times of washing. According to the prepared antibacterial coating material, the synergistic release mode of rapid sterilization and long-acting bacteriostasis is achieved through raw material screening and process optimization, and the short-term and long-acting antibacterial performance can be remarkably improved.

The invention relates to a method for determining pesticide residues, in particular to a method for rapidly separating and quantitatively determining dinotefuran enantiomers in a cucumber sample, an analysis system and application of the dinotefuran enantiomers. The method comprises the following steps: extracting a cucumber sample by using a 1% acetic acid-acetonitrile solution, purifying the cucumber sample by using a Pestii-Carb / PSA solid-phase extraction small column, fixing the volume by using isopropanol / n-heptane (2: 8, v / v), introducing the sample into a UPC2 system equipped with a CHIRALPAK AD-3 chiral chromatographic column, and realizing baseline separation of (+)-(S)-dinotefuran and (-)-(R)-dinotefuran within 4 minutes under the conditions of supercritical CO2 / methanol gradient elution, proper column temperature and back pressure. The method is good in linearity within the range of 0.5-20.0 mg / L, the quantitation limits of the two enantiomers in the cucumber matrix are both 0.1 mg / kg, the adding standard recovery rate is 80.4%-106%, and the relative standard deviation is not larger than 7.5%. The method and the system have the advantages of high analysis speed, low solvent consumption and high sensitivity and precision, and are suitable for conventional monitoring and risk assessment of dinotefuran enantiomer residues in vegetables such as cucumbers and the like.

A wound healing accelerating dressing, its preparation method, and its application belong to the field of functional composite materials. The wound dressing comprises a double-layer structure; the upper layer is an antibacterial adhesive film, prepared by a solution reaction of raw materials including the following components: isophorone diisocyanate, triphenylmethane triisocyanate, hexafluoroisopropanol, polyetheramine 2000, N-methyldiethanolamine, glacial acetic acid, organotin catalyst, and acetone; the lower layer is prepared by a solution reaction of raw materials including the following components: isophorone diisocyanate, polyetheramine 2000, N-methyldiethanolamine, glacial acetic acid, liquid metal, gallic acid, and acetone. This dressing can achieve synergistic effects of protection, antibacterial properties, and electrical stimulation without the need for an external power source, and can be used as a wound healing accelerating dressing that mediates scarless repair, as well as other scar prevention medical materials.

The invention discloses a preparation method of an absorbable antibacterial coating material capable of slowly releasing antibiotics. The preparation method comprises the following steps: step 1, blending antibiotics, an absorbable polymer and a mixed solvent to obtain a coating solution; the antibiotics are selected from rifampicin and minocycline hydrochloride; the absorbable polymer is selected from one or more of polyglycolide, polylactide, polyglycolide-co-lactide, polyglycolide-co-caprolactone and tyrosine polyarylester; the absorbable polymer is selected from one or more of polyglycolide, polylactide, polyglycolide-co-lactide, polyglycolide-co-caprolactone and tyrosine polyarylester; the mixed solvent is selected from hexafluoroisopropanol and acetonitrile, and the volume ratio of the hexafluoroisopropanol in the mixed solvent is not less than 25%; and 2, uniformly spraying the coating solution on the surface of the degradable base material through ultrasonic spraying, and then carrying out drying treatment. According to the preparation method disclosed by the invention, the antibiotic, the absorbable polymer and the organic solvent are blended and dissolved and then are attached to the surface of the absorbable material through ultrasonic spraying, and the release of the antibiotic is regulated and controlled while the stability of the coating is ensured by regulating and controlling the ratio of the antibiotic to the polymer and the swelling characteristic of the solvent to the base material.

This invention provides a glucose-sensitive and antifouling bifunctional probe, its preparation method, and its application, belonging to the field of glucose detection technology. It addresses the problems of poor sensitivity and antifouling stability in existing biological probes used for glucose detection. The preparation method of the glucose-sensitive and antifouling bifunctional probe includes: preparing a phenylboronic acid hydrogel membrane as a glucose-sensitive functionalized probe; mixing 2-bromoisobutyric acid, 3,4-bis(tert-butyl-dimethyl-siloxy)-1-phenylalanine, and a chemical bridge containing a primary amine group, and performing an amidation reaction to obtain an ATRP initiator; mixing an antifouling monomer and the ATRP initiator, and performing an ATRP reaction to obtain an antifouling coating solution; wherein the chemical bridge containing the primary amine group is 1,3-diamino-isopropanol and / or pentaerythritol; and spin-coating the antifouling coating solution onto the surface of the phenylboronic acid hydrogel membrane of the glucose-sensitive functionalized probe to obtain a glucose-sensitive and antifouling bifunctional probe with an antifouling coating. This glucose-sensitive and antifouling bifunctional probe exhibits high sensitivity and antifouling stability.

The application discloses a preparation method of a dual-function antibacterial and anti-inflammatory absorbable coating material, which comprises the following steps: (1) blending rifampicin, artemisinin and an absorbable polymer A with a mixed solvent to obtain coating solution A; blending minocycline hydrochloride, aspirin and an absorbable polymer B with the mixed solvent to obtain coating solution B; the mixed solvent is selected from hexafluoroisopropanol and ethyl acetate; (2) uniformly spraying the coating solution A and the coating solution B on the surface of a degradable substrate by ultrasonic spraying, and performing drying treatment to obtain the dual-function antibacterial and anti-inflammatory absorbable coating material. According to the preparation method, the proportion of antibiotic active ingredients, anti-inflammatory drug active ingredients and absorbable polymers is regulated, the antibacterial and anti-inflammatory drug coating is uniformly and stably fixed on the surface of the degradable substrate by ultrasonic spraying, and the dual-function absorbable antibacterial and anti-inflammatory coating material with controllable release is prepared by optimizing process parameters.

The invention discloses a preparation method of a degradable antibacterial material with slow release performance. The preparation method comprises the following steps: step 1, blending antibiotics and a mixed solvent to obtain an antibiotic solution; the antibiotic is selected from rifampicin and minocycline; the mixed solvent is selected from hexafluoroisopropanol and acetonitrile, and the volume ratio of the hexafluoroisopropanol in the mixed solvent is not less than 25%; step 2, uniformly spraying the antibiotic solution on the surface of a degradable base material through ultrasonic spraying, and then drying to obtain the degradable antibacterial material with the slow release performance. According to the preparation method disclosed by the invention, by regulating and controlling the composition of the mixed solvent and ultrasonic spraying parameters and utilizing the controllable swelling effect of the mixed solvent on the degradable base material, antibiotic molecules are embedded into the surface layer of the material in the atomization spraying process, so that the interface bonding force between the medicine and the degradable base material is remarkably enhanced, and the burst release behavior is effectively inhibited; the continuous and stable in-vitro release performance is realized.