39 results about "Isoprodian" patented technology

The invention belongs to the field of microbiology and molecular biology, and particularly relates to a method for extracting myxococcus flavus dormant mucospore RNA (Ribonucleic Acid). The method specifically comprises the following steps: taking a certain amount of myxococcus flavus liquid, and inducing sporocarp development by using a common starvation induction method; scraping the formed sporocarp structure, resuspending with sterile water, and destroying the sporocarp structure and mucospore coating by using a certain proportion of organic reagent; centrifuging, taking a water phase, adding an acetate buffer solution with a certain concentration, and precipitating with isopropanol to obtain nucleic acid substances; and removing DNA by using a conventional kit to obtain RNA. Aiming at the characteristic that the mucospore RNA is difficult to extract, the invention develops a simple, convenient and efficient method for extracting the mucospore RNA, and provides an effective way for deeply researching the ecological characteristics of the myxobacteria.

The present invention relates to a composition containing (a) a guanidine and / or an unsubstituted guanidine salt, and (b) at least one further component selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, 1, 2-benzisothiazolin-3-one, N-butyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, the cleaning agent is prepared from 2-benzisothiazolin-3-one, octyl isothiazolinone, bronopol, dibromo-nitrilopropionamide, dibromo-dicyanobutane, butyl carbamic acid iodopropynyl ester, aminomethyl propanol, aminoethyl propylene glycol, monoethanolamine, ethylhexylglycerin, hexylglycerin, 1, 2-pentanediol, 1, 2-hexanediol, 1, 2-heptanediol, 1, 2-octylene glycol, phenoxy ethanol and phenethyl alcohol. The cleaning agent is prepared from phenyl propanol, benzyl alcohol, isopropanol, ethylenediamine tetraacetic acid, (1-hydroxyethylidene) diphosphonic acid, 2-mercaptopyridine sodium oxide, 2-mercaptopyridine zinc oxide, o-phenylphenol, sorbic acid, benzoic acid, salicylic acid, lactic acid, citric acid and zinc. The invention also relates to an industrial product containing components (a) and (b), and to the use of said composition for the preservation of containers and for the preservation of industrial products. The invention also relates to the use of (a) a guanidine and / or an unsubstituted guanidine salt for improving the antimicrobial efficacy of one or more compounds selected from the group (b) described above.

The invention discloses a method for rapidly identifying harmful components in pickled peppers, and belongs to the technical field of food detection. The method comprises the following steps: preparing a reference substance solution, namely preparing a standard substance stock solution and a biogenic amine mixed solution from putrescine, histamine, tyramine and cadaverine standard substances; preparing a test sample solution: preparing the test sample solution by adopting a test sample; the chromatographic conditions are as follows: C18 is used as a filling agent; carrying out gradient elution by taking isopropanol as a mobile phase A and 0.5-2wt% ammonia water as a mobile phase B; the detection wavelength is 254 nm; the column temperature is 25-35 DEG C; and the flow velocity is 0.2-1.0 ml / min. The method has the advantages that (1) the detection speed is high, and the gradient elution speed is high and is only 12 minutes; (2) the number of obvious miscellaneous peaks is small, and only one miscellaneous peak exists; and (3) the method has relatively high precision, relatively low detection limit and good reproducibility.

The invention discloses a method for efficiently extracting genome DNA (Deoxyribose Nucleic Acid) of ophiurocephalus serratus. The method comprises the following steps: mixing a sample with a lysis solution and metal balls, crushing the mixture at-20 to-10 DEG C for 250-350s at 40-60Hz, and placing the crushed sample at-20 to 4 DEG C; cracking the sample at the temperature of 50-60 DEG C for 4-6 minutes; centrifuging at 2-4 DEG C, taking a supernatant, adding an extraction agent, and uniformly mixing; centrifuging at 2-4 DEG C, taking an upper-layer solution, adding an extracting agent, and uniformly mixing; centrifuging at 2-4 DEG C, taking an upper-layer solution, adding isopropanol, uniformly mixing, and standing at-20--15 DEG C for 20-30 minutes; centrifuging at 2-4 DEG C, discarding the supernatant, washing the precipitate with an ethanol solution, and dissolving with an eluent; the lysis solution contains CTAB (Cetyltrimethyl Ammonium Bromide), protease K and beta-mercaptoethanol. According to the invention, the problem of high-mineralization echinodermis sample treatment is solved, and a high-quality nucleic acid template and a new technology are provided for research on ocean invertebrate genetics.

The invention discloses a corn seed RNA (Ribonucleic Acid) extraction method which comprises the following steps: adding a corn seed sample ground by liquid nitrogen into a sample tube, adding grinding beads and an extraction buffer solution preheated at 60-65 DEG C, and uniformly mixing by vortex; the extraction buffer solution is prepared from 90 to 100 mM of Tris-HCl, 20 to 25 mM of EDTA (Ethylene Diamine Tetraacetic Acid), 1 to 2 percent of CTAB (Cetyltrimethyl Ammonium Bromide), 0.5 to 1 M of NaCl and 1 to 2 percent of beta-mercaptoethanol; adding a phenol-chloroform mixed solution, vigorously shaking, centrifuging, taking an upper-layer solution into a centrifugal tube, adding isometric isopropanol, sufficiently and uniformly mixing, adding a magnetic bead mixed solution, performing magnetic bead purification, and extracting to obtain the corn seed RNA. Reagent consumables used in the method are simple and easy to obtain, and high-quality and high-purity corn seed RNA can be obtained in a short time. Meanwhile, the method utilizes magnetic bead purification and is combined with related equipment, so that the extraction flux can be greatly improved.

This invention discloses a purification method for Tirzepatide. The method includes: dissolving crude Tirzepatide peptide in ammonia or sodium bicarbonate solution, filtering, and purifying by reversed-phase chromatography to obtain a purified sample; eluting the purified sample using reversed-phase high-performance liquid chromatography (RP-HPLC) with a sodium or potassium buffer solution as mobile phase A3 and one or a mixture of acetonitrile, methanol, and isopropanol in any proportion as mobile phase B, preparing a sodium or potassium salt fraction; cooling the sodium or potassium salt fraction to -20 to 10°C and maintaining the temperature; then adding an organic solvent to the fraction, controlling the temperature at -20 to 10°C to precipitate the sample; after complete precipitation, solid-liquid separation is performed, the solid is collected, dissolved in water, filtered, and freeze-dried. This invention improves the concentration speed, ensures product purity, and strictly controls impurities.

The invention discloses a bridged ring phloroglucinol antibacterial compound as well as a preparation method and application thereof, and belongs to the field of medicinal chemistry and anti-infection pharmacy. The structural general formula of the bridged ring phloroglucinol antibacterial compound is shown in the specification. The preparation method specifically comprises the following steps: (1) preparing a compound B by taking methyl iodide, sodium methoxide and a compound A as raw materials; (2) preparing a compound C by taking diisobutylaluminium hydride, the compound B, 2, 6-dimethyl pyridine and trifluoromethanesulfonic anhydride as raw materials; (3) preparing a compound D by taking palladium acetate, triphenylphosphine, the compound C, triethylamine and formic acid as raw materials; and (4) preparing a target product by taking the compound D, hexafluoroisopropanol, p-toluenesulfonic acid, the compound E, chiral phosphoric acid and ytterbium trifluoromethanesulfonate as raw materials. The compound disclosed by the invention shows high selectivity, low drug resistance risk and a potential new action mechanism, and provides a new leading structure and a technical path for overcoming drug resistance of existing antibacterial drugs.

The invention discloses a method for detecting an Inclisiran drug in a sample. The method comprises the following steps: analyzing and detecting a to-be-detected sample by adopting a liquid chromatography-mass spectrometry method, a mobile phase adopted by liquid chromatography in the liquid chromatography-mass spectrometry method comprises a mobile phase A, the mobile phase A is a mixed solution of water and methanol, and the mobile phase A also contains 0.1-2.0% (v / v) of hexafluoroisopropanol and 0.1-1.0% (v / v) of N, N-dimethylformamide; the solvent is N, N-diisopropylethylamine and ethylene diamine tetraacetic acid with the concentration of 0.1-20 [mu] M. The detection method disclosed by the invention is high in sensitivity and low in detection limit, and can be used for realizing the analysis of pharmacokinetics / toxicokinetics of the Inclisiran medicine in a sample to be detected, such as Sprague Dawley rat EDTA-K2 plasma.

This invention relates to the field of fecal genomics kit technology, and particularly to a safe, rapid, and high-throughput kit for extracting fecal genomes and its usage method. The kit includes a sample preservation solution, magnetic beads, a lysis buffer, a washing buffer 1, a washing buffer 2, and an elution buffer. The sample preservation solution is composed of guanidine isothiocyanate, sodium citrate, Triton X-100, Tween 20, propidium iodide, and purified water. The magnetic beads are hydroxyl magnetic beads. The lysis buffer is composed of guanidine hydrochloride, Tris base, Tris hydrochloric acid, Triton X-100, isopropanol, and purified water. Washing buffer 1 is composed of guanidine hydrochloride solution and isopropanol. Washing buffer 2 is composed of anhydrous ethanol and purified water. This kit can easily extract the genome from fecal samples, is simple to operate, safe and non-toxic, and can extract multiple samples at once, enabling rapid diagnosis and filling the gap in the current market for automated high-throughput kits for extracting genomes from fecal samples.

The invention discloses a method for removing endotoxin by hydrophobic chromatography, which comprises the following specific steps: adding TritonX-114 into a protein sample to be subjected to endotoxin removal, and stirring at 0-4 DEG C to completely dissolve the TritonX-114 to prepare a sample to be detected; sequentially cleaning the filler in the hydrophobic chromatographic column with isopropanol, water and sodium hydroxide, and balancing the hydrophobic chromatographic column with a buffer solution; loading the sample to be detected into the balanced hydrophobic chromatographic column, and collecting a flow-through sample containing target protein; and cleaning the filler in the hydrophobic chromatographic column by using the buffer solution again. According to the method, the endotoxin is removed by using the hydrophobicity of the endotoxin by using a hydrophobic chromatography method, the endotoxin residue in the protein raw material can be reduced by 90% or more, the protein loss is less, the residual amount of TritonX-114 in the harvested protein sample is less, the influence of the TritonX-114 residue on downstream use is reduced, and the method has the characteristics of simple process and easiness in operation, and is suitable for industrial production. And the method is suitable for large-scale production.

The present invention provides a medical waste disinfectant and a preparation method and a use method thereof, and relates to the field of disinfection technology. The medical waste disinfectant comprises: actinomycin D with a mass fraction of 0.01-0.05%, dodecyl dimethyl benzyl ammonium chloride with a mass fraction of 0.05-0.1%, didodecyl dimethyl ammonium chloride with a mass fraction of 0.01-0.05%, sodium nitrite with a mass fraction of 0.4-0.6%, isopropanol with a mass fraction of 0.05-0.1%, pine oil with a mass fraction of 0.01-0.05%, and the pH of the disinfectant is 6.8-7.2. The disinfectant can effectively sterilize medical waste, has low corrosivity to equipment, and has low product toxicity.

The present invention is applicable to the field of biotechnology and provides a method for extracting Helicobacter pylori DNA from feces, which comprises the following steps: collecting a fresh fecal sample of a patient infected with Helicobacter pylori and placing it in a centrifuge tube; adding a cell lysate to the centrifuge tube and performing homogenization. The cell lysate includes: Tris-HCl, ethylenediaminetetraacetic acid, guanidine isothiocyanate, Triton X-100, isopropanol, sodium dodecyl sulfate, sodium chloride, proteinase K, modified polyethylene glycol, and modified betaine. By adding modified polyethylene glycol to the cell lysate, the capture of free DNA is enhanced, promoting further concentration and enrichment of DNA and improving the subsequent binding efficiency of DNA to magnetic beads. By adding modified betaine, the effects of removing impurities such as proteins and esters are improved, reducing the impurities adsorbed by magnetic beads in the subsequent steps. Modified polyethylene glycol and modified betaine have a synergistic effect and can improve the purity of the extracted DNA.

The invention discloses a plasma free DNA extraction reagent, a kit and an extraction method, the extraction reagent comprises protease K, a lysis solution, a binding solution, a washing solution 1, a washing solution 2, a washing solution 3 and an eluent, the extraction reagents contain more common and less-dosage common reagents such as Tris-HCl (pH 8.0), EDTA.2Na. 2H2O, NaCl, glycerol, SDS, isopropanol, ethanol, Tween-20 and the like. The method has the advantages that the problems that the reagent is easy to separate out and needs to be heated and redissolved for use during storage can be solved without traditional high-concentration guanidine salts such as guanidine hydrochloride and guanidinium isothiocyanate, the stability and the operation convenience of the extraction reagent are increased, a better extraction effect is achieved, and the eluent can effectively protect the extracted cfDNA from being degraded.

The invention provides a magnetic bead method liquid sample pathogenic microorganism nucleic acid extraction kit, which is characterized by comprising protease K, a nucleic acid protection solution, a lysis strengthening solution, a lysis binding solution, magnetic beads MXVB, a washing solution 1, a washing solution 2 and an eluent, wherein the cracking combination liquid is prepared from tris (hydroxymethyl) aminomethane, guanidine isothiocyanate, ammonium thiocyanate, tween-80, lauryl sodium sulfate, sodium chloride and isopropanol; the nucleic acid protection liquid comprises tris (hydroxymethyl) aminomethane hydrochloride and formamide; the cracking reinforcing liquid is lauryl sodium sulfate. According to the kit, DNA of bacteria, fungi and viruses can be rapidly extracted and purified, the extraction efficiency of pathogenic microorganism nucleic acid is improved, good stability is achieved, the nucleic acid quality is guaranteed, and development of a pathogenic microorganism detection technology is facilitated.

The invention discloses a preparation method of an antibacterial coating material based on synergistic interaction of antibiotics and metal ions. The preparation method comprises the following steps: (1) blending antibiotics, soluble salts of metal ions, an absorbable polymer and a mixed solvent to obtain a coating solution; the antibiotics are selected from rifampicin and minocycline hydrochloride; the metal ions are selected from Zn and / or Cu; the mixed solvent is selected from hexafluoroisopropanol and a methanol / aqueous solution, and the volume ratio of hexafluoroisopropanol in the mixed solvent is not less than 25%; and (2) uniformly spraying the coating solution on the surface of the degradable base material through ultrasonic spraying, and then carrying out drying treatment. The preparation method is easy and convenient to operate, the obtained coating is tightly combined with a degradable base material, and the antibacterial rate can still reach 90% or above after 50 times of washing. According to the prepared antibacterial coating material, the synergistic release mode of rapid sterilization and long-acting bacteriostasis is achieved through raw material screening and process optimization, and the short-term and long-acting antibacterial performance can be remarkably improved.

The invention relates to a method for determining pesticide residues, in particular to a method for rapidly separating and quantitatively determining dinotefuran enantiomers in a cucumber sample, an analysis system and application of the dinotefuran enantiomers. The method comprises the following steps: extracting a cucumber sample by using a 1% acetic acid-acetonitrile solution, purifying the cucumber sample by using a Pestii-Carb / PSA solid-phase extraction small column, fixing the volume by using isopropanol / n-heptane (2: 8, v / v), introducing the sample into a UPC2 system equipped with a CHIRALPAK AD-3 chiral chromatographic column, and realizing baseline separation of (+)-(S)-dinotefuran and (-)-(R)-dinotefuran within 4 minutes under the conditions of supercritical CO2 / methanol gradient elution, proper column temperature and back pressure. The method is good in linearity within the range of 0.5-20.0 mg / L, the quantitation limits of the two enantiomers in the cucumber matrix are both 0.1 mg / kg, the adding standard recovery rate is 80.4%-106%, and the relative standard deviation is not larger than 7.5%. The method and the system have the advantages of high analysis speed, low solvent consumption and high sensitivity and precision, and are suitable for conventional monitoring and risk assessment of dinotefuran enantiomer residues in vegetables such as cucumbers and the like.

The invention provides a preparation process of a Hep-2 cell extract suitable for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies and a preparation method of the test strip. The method comprises the following steps: transferring cultured Hep-2 cells into a PBS (Phosphate Buffer Solution), carrying out centrifugal separation on the obtained Hep-2 cell suspension to obtain Hep-2 cell agglomerates, carrying out ultrasonic treatment on a mixture of the Hep-2 cell agglomerates and the extraction buffer solution in an ice bath, centrifuging the mixture subjected to ultrasonic treatment, collecting supernatant, and passing the supernatant through a micro-filtration membrane to obtain filtrate, namely the Hep-2 cell extract. The extraction buffer solution is an HEPES buffer solution containing isopropanol, trehalose, bovine serum albumin and phenylmethylsulfonyl fluoride. The prepared HEP-2 cell extract is used for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies, and the detection sensitivity, the specificity and the reliability of a detection result can be considered at the same time.

The invention provides a medical endoscope efficient sterilization disinfectant and a preparation method thereof.The medical endoscope efficient sterilization disinfectant is formed by mixing an endoscope disinfectant A and an endoscope disinfectant B. The endoscope disinfectant A comprises, by weight, 0.03%-0.1% of hydrogen peroxide stabilizer, 0.1%-0.5% of sodium gluconate, 0.1%-0.5% of sorbitol, 0.02%-0.2% of disodium hydrogen phosphate, 8.5%-20.0% of hydrogen peroxide and the balance water. And the balance of purified water. The endoscope disinfection liquid B is prepared from the following components in percentage by weight: 55 to 70 percent of N-acetylcaprolactam, 20 to 30 percent of isopropanol and 5.0 to 15.0 percent of ethyl acetate. The sterilizing and disinfecting liquid can effectively sterilize and disinfect medical endoscopes and has the advantages of being high in stability, long in validity period, high in sterilizing and disinfecting efficiency and the like.

The invention relates to a method for efficiently extracting nucleic acid from fresh milk, which belongs to the technical field of bioengineering application, and is characterized by comprising the following steps: (1) adding normal saline into a fresh milk sample for the second time, mixing and centrifuging, and discarding a fat layer and supernatant liquid to obtain a precipitate A; (2) adding a TES solution, a guanidine hydrochloride solution and a protease K solution into the precipitate A; cooling at room temperature after water bath, adding Tris saturated phenol, extracting and centrifuging, and taking supernate B; and (3) adding a sodium acetate solution and isopropanol into the supernate B, mixing and centrifuging, discarding the supernate to obtain nucleic acid, dissolving the nucleic acid in double distilled water, and preserving at-20 DEG C for later use. The method can effectively eliminate the influence of fat and protein on nucleic acid extraction, and has the characteristics that nucleic acid is conveniently and effectively extracted from various fresh milk, the concentration and purity of the extracted nucleic acid are high, and the method is simple.

Methods and kits for determining the amount of coenzyme Q10 in a biological sample are provided. Relates to the technical field of medical biology. The method comprises the following steps: taking a biological sample to be determined, adding a 1, 4-benzoquinone solution and an internal standard substance, adding an extracting agent, extracting, taking an extracting solution, filtering, and performing liquid chromatography-tandem mass spectrometry determination, wherein the chromatographic conditions are as follows: a Waters C18 chromatographic column is adopted, and a 100% B phase is used as a mobile phase for elution; the mobile phase comprises 0.1% by volume of a component 1 and 99.9% by volume of a component 2, the component 1 is formic acid, and the component 2 is composed of methanol and isopropanol in a ratio of (7-9): (3-1). According to the embodiment of the invention, parameters are optimized through fuzzy logic, the efficient, accurate, sensitive and low-cost method for measuring the amount of the coenzyme Q10 in the biological sample is established, the mobile phase selection of LC-MS / MS detection of the CoQ10 is optimized by virtue of the capability of processing uncertain data through the fuzzy logic, and the precision of the method is remarkably improved compared with that of similar researches.

A wound healing accelerating dressing, its preparation method, and its application belong to the field of functional composite materials. The wound dressing comprises a double-layer structure; the upper layer is an antibacterial adhesive film, prepared by a solution reaction of raw materials including the following components: isophorone diisocyanate, triphenylmethane triisocyanate, hexafluoroisopropanol, polyetheramine 2000, N-methyldiethanolamine, glacial acetic acid, organotin catalyst, and acetone; the lower layer is prepared by a solution reaction of raw materials including the following components: isophorone diisocyanate, polyetheramine 2000, N-methyldiethanolamine, glacial acetic acid, liquid metal, gallic acid, and acetone. This dressing can achieve synergistic effects of protection, antibacterial properties, and electrical stimulation without the need for an external power source, and can be used as a wound healing accelerating dressing that mediates scarless repair, as well as other scar prevention medical materials.

The present invention provides a sample pretreatment method and kit for extracting 8 catecholamines and their metabolites from urine. The sample pretreatment method adopts a magnetic bead extraction method in combination with a fully automatic extractor for automatic extraction. The magnetic bead working solution in the magnetic bead extraction method is a suspension of magnetic beads, water and isopropanol, and the magnetic beads are a mixture of carboxyl magnetic beads and amino magnetic beads. The sample pretreatment method comprises: S100, the fully automatic extractor sequentially performs sample loading, rinsing and elution steps to obtain a solution to be tested; S200, the solution to be tested is used for LC-MS / MS detection. The technical problem solved by the present invention is that in the prior art, when the magnetic bead extraction method uses a single magnetic bead extraction, the detection of 8 catecholamines and their metabolites requires two sample pretreatments and two tests to complete, resulting in low throughput, poor recovery rate, and cumbersome operation, making it difficult to meet the needs of clinical high throughput.

The invention relates to the technical field of quality standard detection of traditional Chinese medicinal materials, in particular to a quality detection method of egg butter. According to the method, beta-sitosterol is used as a quality detection index of the egg butter, and quality evaluation can be better carried out on the skin repair effect of the egg butter for treating burns and scalds. The invention newly establishes a thin-layer chromatography identification method taking beta-sitosterol as a contrast, and the method has the advantages of uniform color spot shape, good separation and clear color development. In addition, a high performance liquid chromatography method is adopted, silica gel is used as a stationary phase, and a normal-phase chromatographic column (SiO2, 250 * 4.6 mm) is adopted; n-hexane-petroleum ether (30-60 DEG C)-isopropanol (98: 1: 1) is used as a mobile phase; the detection wavelength is 210 nm, the column temperature is 20 DEG C, the flow velocity is 1.0 ml / min, and 10 microliters of sample is injected, so that the determination effect is better, and the repeatability, the stability and the precision are good.

(I) (II) A primer comprises a carrier and compound (I) or (II), wherein R1 to R11 are independently selected from H, OH, halogen, CH3, linear or branched C2-C6 alkyl, linear or branched C2-C6 alkoxy, linear or branched C2-C6 alkenyl, C2-C6 alkynyl, C3-C9 cycloalkyl, C4-C9 cycloalkenyl, C4-C9 cycloalkynyl, C3-C9 aryl, C3-C9 heteroaryl, C3-C9 heteroalicyclyl, C6-C12 aralkyl, C6- C12 heteroaralkyl, C6-C12 (heteroalicyclyl)alkyl, hydroxy, protected hydroxyl, C2-C6 alkoxy, C4-C6 aryloxy, C2-C6 acyl, ester, mercapto, alkylthio, arylthio, cyano, halogen, carbonyl, thiocarbonyl, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulphonamido, N-sulphonamido, C-carboxy, protected C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, nitro, silyl, sulphenyl, sulphinyl, sulphonyl, haloalkyl, haloalkoxy, trihalomethanesulphonyl, trihalomethanesulphonamido, and amino. Preferably, the compound is 4-hydroxybenzoic acid, orcinol, trihydroxybenzene, 3-hydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, 4-hydroxy-2-methylbenzoic acid, or 4-fluoro-2-methylbenzoic acid. The compound may be present in an amount of 0.1-10 wt.%. Typically, the carrier is isopropyl alcohol. A method of bonding substrates using the primer and an adhesive is disclosed.

The present invention belongs to the technical field of drug analysis, and in particular to a method for determining residual solvents in LXH-2103 using headspace gas chromatography. The determination method of the present invention comprises the following steps: preparing a blank solution, a reference solution, and a test solution, injecting the blank solution, the reference solution, and the sample solution into a headspace equilibrium, injecting the blank solution, the reference solution, and the sample solution into a gas chromatograph by headspace injection, recording the chromatogram, and calculating the content by peak area according to the external standard method; the residual solvent is anhydrous methanol, anhydrous ethanol, isopropanol, dichloromethane, or toluene. The method for determining residual solvents in LXH-2103 using headspace gas chromatography provided by the present invention has high sensitivity, good repeatability, high accuracy, and can simultaneously detect multiple residual solvents in LXH-2103, thereby ensuring the quality of LXH-2103 and improving the safety of clinical medication.

The invention discloses a kit and a method for extracting DNA (deoxyribonucleic acid) in edible oil. The kit comprises a release unit, an adsorption unit and an enrichment unit, the release unit comprises a TE buffer solution, Tween 20, 3-[(3-cholic acid acyloxy) propyl] dimethylamino-1-propanesulfonic acid and sodium chloride; the adsorption unit comprises a PEI nanofiber membrane and guanidine hydrochloride; and the enrichment unit comprises polyethylene glycol 8000 and isopropanol. The DNA can be effectively extracted from the refined edible oil through the kit and the matched extraction method. The kit and the extraction method provided by the invention can provide a reliable DNA template basis for molecular detection technologies such as edible oil molecule traceability, adulteration detection, transgenic screening and the like. Meanwhile, the extraction method is simple and easy to operate, environment-friendly and non-toxic in extraction process, high in extraction efficiency and suitable for standardized popularization and application. Therefore, the kit and the extraction method have good application prospects and industrialization potential.

The invention discloses a preparation method of an absorbable antibacterial coating material capable of slowly releasing antibiotics. The preparation method comprises the following steps: step 1, blending antibiotics, an absorbable polymer and a mixed solvent to obtain a coating solution; the antibiotics are selected from rifampicin and minocycline hydrochloride; the absorbable polymer is selected from one or more of polyglycolide, polylactide, polyglycolide-co-lactide, polyglycolide-co-caprolactone and tyrosine polyarylester; the absorbable polymer is selected from one or more of polyglycolide, polylactide, polyglycolide-co-lactide, polyglycolide-co-caprolactone and tyrosine polyarylester; the mixed solvent is selected from hexafluoroisopropanol and acetonitrile, and the volume ratio of the hexafluoroisopropanol in the mixed solvent is not less than 25%; and 2, uniformly spraying the coating solution on the surface of the degradable base material through ultrasonic spraying, and then carrying out drying treatment. According to the preparation method disclosed by the invention, the antibiotic, the absorbable polymer and the organic solvent are blended and dissolved and then are attached to the surface of the absorbable material through ultrasonic spraying, and the release of the antibiotic is regulated and controlled while the stability of the coating is ensured by regulating and controlling the ratio of the antibiotic to the polymer and the swelling characteristic of the solvent to the base material.

The present invention provides a solid form of a compound of formula (I) or a solvate thereof having autotaxin (ATX) inhibitory activity, wherein the solid form comprises a crystalline form and / or an amorphous form. The crystalline form comprises two non-solvate crystalline forms: crystalline form A and crystalline form L; six hydrate crystalline forms: crystalline form B (dihydrate), crystalline form D (trihydrate), crystalline form G (tetrahydrate), crystalline form H (monohydrate), crystalline form K (dihydrate) and crystalline form M-1 (monohydrate); and seven solvate crystalline forms: crystalline form C (hemitoluene solvate), crystalline form E (chloroform solvate), crystalline form N (dichloromethane solvate), crystalline form P (isopropanol solvate), crystalline form J-1 (hemisec-butyltetrahydrofuran solvate), crystalline form J-2 (methyl isobutyl ketone solvate) and crystalline form M-2 (acetonitrile solvate). The solid form can be used for treating and / or preventing diseases related to autotaxin (ATX).

This invention provides a glucose-sensitive and antifouling bifunctional probe, its preparation method, and its application, belonging to the field of glucose detection technology. It addresses the problems of poor sensitivity and antifouling stability in existing biological probes used for glucose detection. The preparation method of the glucose-sensitive and antifouling bifunctional probe includes: preparing a phenylboronic acid hydrogel membrane as a glucose-sensitive functionalized probe; mixing 2-bromoisobutyric acid, 3,4-bis(tert-butyl-dimethyl-siloxy)-1-phenylalanine, and a chemical bridge containing a primary amine group, and performing an amidation reaction to obtain an ATRP initiator; mixing an antifouling monomer and the ATRP initiator, and performing an ATRP reaction to obtain an antifouling coating solution; wherein the chemical bridge containing the primary amine group is 1,3-diamino-isopropanol and / or pentaerythritol; and spin-coating the antifouling coating solution onto the surface of the phenylboronic acid hydrogel membrane of the glucose-sensitive functionalized probe to obtain a glucose-sensitive and antifouling bifunctional probe with an antifouling coating. This glucose-sensitive and antifouling bifunctional probe exhibits high sensitivity and antifouling stability.

The present invention provides a kind of Hep 2 cell extract preparation process and preparation method of test strip suitable for preparing antinuclear antibody fluorescent immunochromatographic test strip. The present invention transfers the Hep 2 cells obtained by culture into PBS buffer, and the obtained Hep 2 cell suspension is centrifuged to obtain Hep 2 cell agglomerates, and the mixture of Hep 2 cell agglomerates and extraction buffer is ultrasonicated in an ice bath, and the mixture after ultrasonication is centrifuged, and the supernatant is collected, and the supernatant is passed through a microfiltration membrane. The obtained filtrate is the Hep 2 cell extract, and the extraction buffer is a HEPES buffer containing isopropanol, trehalose, bovine serum albumin and phenylmethylsulfonyl fluoride. The prepared HEP 2 cell extract is used for preparing antinuclear antibody fluorescent immunochromatographic test strip, which can take into account the reliability of detection sensitivity, specificity and test results.