19 results about "Isoprodian" patented technology

The invention belongs to the field of microbiology and molecular biology, and particularly relates to a method for extracting myxococcus flavus dormant mucospore RNA (Ribonucleic Acid). The method specifically comprises the following steps: taking a certain amount of myxococcus flavus liquid, and inducing sporocarp development by using a common starvation induction method; scraping the formed sporocarp structure, resuspending with sterile water, and destroying the sporocarp structure and mucospore coating by using a certain proportion of organic reagent; centrifuging, taking a water phase, adding an acetate buffer solution with a certain concentration, and precipitating with isopropanol to obtain nucleic acid substances; and removing DNA by using a conventional kit to obtain RNA. Aiming at the characteristic that the mucospore RNA is difficult to extract, the invention develops a simple, convenient and efficient method for extracting the mucospore RNA, and provides an effective way for deeply researching the ecological characteristics of the myxobacteria.

The present invention relates to a composition containing (a) a guanidine and / or an unsubstituted guanidine salt, and (b) at least one further component selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, 1, 2-benzisothiazolin-3-one, N-butyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, 2-benzisothiazolin-3-one, N-methyl-1, the cleaning agent is prepared from 2-benzisothiazolin-3-one, octyl isothiazolinone, bronopol, dibromo-nitrilopropionamide, dibromo-dicyanobutane, butyl carbamic acid iodopropynyl ester, aminomethyl propanol, aminoethyl propylene glycol, monoethanolamine, ethylhexylglycerin, hexylglycerin, 1, 2-pentanediol, 1, 2-hexanediol, 1, 2-heptanediol, 1, 2-octylene glycol, phenoxy ethanol and phenethyl alcohol. The cleaning agent is prepared from phenyl propanol, benzyl alcohol, isopropanol, ethylenediamine tetraacetic acid, (1-hydroxyethylidene) diphosphonic acid, 2-mercaptopyridine sodium oxide, 2-mercaptopyridine zinc oxide, o-phenylphenol, sorbic acid, benzoic acid, salicylic acid, lactic acid, citric acid and zinc. The invention also relates to an industrial product containing components (a) and (b), and to the use of said composition for the preservation of containers and for the preservation of industrial products. The invention also relates to the use of (a) a guanidine and / or an unsubstituted guanidine salt for improving the antimicrobial efficacy of one or more compounds selected from the group (b) described above.

The invention discloses a method for efficiently extracting genome DNA (Deoxyribose Nucleic Acid) of ophiurocephalus serratus. The method comprises the following steps: mixing a sample with a lysis solution and metal balls, crushing the mixture at-20 to-10 DEG C for 250-350s at 40-60Hz, and placing the crushed sample at-20 to 4 DEG C; cracking the sample at the temperature of 50-60 DEG C for 4-6 minutes; centrifuging at 2-4 DEG C, taking a supernatant, adding an extraction agent, and uniformly mixing; centrifuging at 2-4 DEG C, taking an upper-layer solution, adding an extracting agent, and uniformly mixing; centrifuging at 2-4 DEG C, taking an upper-layer solution, adding isopropanol, uniformly mixing, and standing at-20--15 DEG C for 20-30 minutes; centrifuging at 2-4 DEG C, discarding the supernatant, washing the precipitate with an ethanol solution, and dissolving with an eluent; the lysis solution contains CTAB (Cetyltrimethyl Ammonium Bromide), protease K and beta-mercaptoethanol. According to the invention, the problem of high-mineralization echinodermis sample treatment is solved, and a high-quality nucleic acid template and a new technology are provided for research on ocean invertebrate genetics.

This invention discloses a purification method for Tirzepatide. The method includes: dissolving crude Tirzepatide peptide in ammonia or sodium bicarbonate solution, filtering, and purifying by reversed-phase chromatography to obtain a purified sample; eluting the purified sample using reversed-phase high-performance liquid chromatography (RP-HPLC) with a sodium or potassium buffer solution as mobile phase A3 and one or a mixture of acetonitrile, methanol, and isopropanol in any proportion as mobile phase B, preparing a sodium or potassium salt fraction; cooling the sodium or potassium salt fraction to -20 to 10°C and maintaining the temperature; then adding an organic solvent to the fraction, controlling the temperature at -20 to 10°C to precipitate the sample; after complete precipitation, solid-liquid separation is performed, the solid is collected, dissolved in water, filtered, and freeze-dried. This invention improves the concentration speed, ensures product purity, and strictly controls impurities.

The invention discloses a bridged ring phloroglucinol antibacterial compound as well as a preparation method and application thereof, and belongs to the field of medicinal chemistry and anti-infection pharmacy. The structural general formula of the bridged ring phloroglucinol antibacterial compound is shown in the specification. The preparation method specifically comprises the following steps: (1) preparing a compound B by taking methyl iodide, sodium methoxide and a compound A as raw materials; (2) preparing a compound C by taking diisobutylaluminium hydride, the compound B, 2, 6-dimethyl pyridine and trifluoromethanesulfonic anhydride as raw materials; (3) preparing a compound D by taking palladium acetate, triphenylphosphine, the compound C, triethylamine and formic acid as raw materials; and (4) preparing a target product by taking the compound D, hexafluoroisopropanol, p-toluenesulfonic acid, the compound E, chiral phosphoric acid and ytterbium trifluoromethanesulfonate as raw materials. The compound disclosed by the invention shows high selectivity, low drug resistance risk and a potential new action mechanism, and provides a new leading structure and a technical path for overcoming drug resistance of existing antibacterial drugs.

This invention relates to the field of fecal genomics kit technology, and particularly to a safe, rapid, and high-throughput kit for extracting fecal genomes and its usage method. The kit includes a sample preservation solution, magnetic beads, a lysis buffer, a washing buffer 1, a washing buffer 2, and an elution buffer. The sample preservation solution is composed of guanidine isothiocyanate, sodium citrate, Triton X-100, Tween 20, propidium iodide, and purified water. The magnetic beads are hydroxyl magnetic beads. The lysis buffer is composed of guanidine hydrochloride, Tris base, Tris hydrochloric acid, Triton X-100, isopropanol, and purified water. Washing buffer 1 is composed of guanidine hydrochloride solution and isopropanol. Washing buffer 2 is composed of anhydrous ethanol and purified water. This kit can easily extract the genome from fecal samples, is simple to operate, safe and non-toxic, and can extract multiple samples at once, enabling rapid diagnosis and filling the gap in the current market for automated high-throughput kits for extracting genomes from fecal samples.

The invention discloses a plasma free DNA extraction reagent, a kit and an extraction method, the extraction reagent comprises protease K, a lysis solution, a binding solution, a washing solution 1, a washing solution 2, a washing solution 3 and an eluent, the extraction reagents contain more common and less-dosage common reagents such as Tris-HCl (pH 8.0), EDTA.2Na. 2H2O, NaCl, glycerol, SDS, isopropanol, ethanol, Tween-20 and the like. The method has the advantages that the problems that the reagent is easy to separate out and needs to be heated and redissolved for use during storage can be solved without traditional high-concentration guanidine salts such as guanidine hydrochloride and guanidinium isothiocyanate, the stability and the operation convenience of the extraction reagent are increased, a better extraction effect is achieved, and the eluent can effectively protect the extracted cfDNA from being degraded.

The invention discloses a preparation method of an antibacterial coating material based on synergistic interaction of antibiotics and metal ions. The preparation method comprises the following steps: (1) blending antibiotics, soluble salts of metal ions, an absorbable polymer and a mixed solvent to obtain a coating solution; the antibiotics are selected from rifampicin and minocycline hydrochloride; the metal ions are selected from Zn and / or Cu; the mixed solvent is selected from hexafluoroisopropanol and a methanol / aqueous solution, and the volume ratio of hexafluoroisopropanol in the mixed solvent is not less than 25%; and (2) uniformly spraying the coating solution on the surface of the degradable base material through ultrasonic spraying, and then carrying out drying treatment. The preparation method is easy and convenient to operate, the obtained coating is tightly combined with a degradable base material, and the antibacterial rate can still reach 90% or above after 50 times of washing. According to the prepared antibacterial coating material, the synergistic release mode of rapid sterilization and long-acting bacteriostasis is achieved through raw material screening and process optimization, and the short-term and long-acting antibacterial performance can be remarkably improved.

The invention relates to a method for determining pesticide residues, in particular to a method for rapidly separating and quantitatively determining dinotefuran enantiomers in a cucumber sample, an analysis system and application of the dinotefuran enantiomers. The method comprises the following steps: extracting a cucumber sample by using a 1% acetic acid-acetonitrile solution, purifying the cucumber sample by using a Pestii-Carb / PSA solid-phase extraction small column, fixing the volume by using isopropanol / n-heptane (2: 8, v / v), introducing the sample into a UPC2 system equipped with a CHIRALPAK AD-3 chiral chromatographic column, and realizing baseline separation of (+)-(S)-dinotefuran and (-)-(R)-dinotefuran within 4 minutes under the conditions of supercritical CO2 / methanol gradient elution, proper column temperature and back pressure. The method is good in linearity within the range of 0.5-20.0 mg / L, the quantitation limits of the two enantiomers in the cucumber matrix are both 0.1 mg / kg, the adding standard recovery rate is 80.4%-106%, and the relative standard deviation is not larger than 7.5%. The method and the system have the advantages of high analysis speed, low solvent consumption and high sensitivity and precision, and are suitable for conventional monitoring and risk assessment of dinotefuran enantiomer residues in vegetables such as cucumbers and the like.

The invention provides a medical endoscope efficient sterilization disinfectant and a preparation method thereof.The medical endoscope efficient sterilization disinfectant is formed by mixing an endoscope disinfectant A and an endoscope disinfectant B. The endoscope disinfectant A comprises, by weight, 0.03%-0.1% of hydrogen peroxide stabilizer, 0.1%-0.5% of sodium gluconate, 0.1%-0.5% of sorbitol, 0.02%-0.2% of disodium hydrogen phosphate, 8.5%-20.0% of hydrogen peroxide and the balance water. And the balance of purified water. The endoscope disinfection liquid B is prepared from the following components in percentage by weight: 55 to 70 percent of N-acetylcaprolactam, 20 to 30 percent of isopropanol and 5.0 to 15.0 percent of ethyl acetate. The sterilizing and disinfecting liquid can effectively sterilize and disinfect medical endoscopes and has the advantages of being high in stability, long in validity period, high in sterilizing and disinfecting efficiency and the like.

Methods and kits for determining the amount of coenzyme Q10 in a biological sample are provided. Relates to the technical field of medical biology. The method comprises the following steps: taking a biological sample to be determined, adding a 1, 4-benzoquinone solution and an internal standard substance, adding an extracting agent, extracting, taking an extracting solution, filtering, and performing liquid chromatography-tandem mass spectrometry determination, wherein the chromatographic conditions are as follows: a Waters C18 chromatographic column is adopted, and a 100% B phase is used as a mobile phase for elution; the mobile phase comprises 0.1% by volume of a component 1 and 99.9% by volume of a component 2, the component 1 is formic acid, and the component 2 is composed of methanol and isopropanol in a ratio of (7-9): (3-1). According to the embodiment of the invention, parameters are optimized through fuzzy logic, the efficient, accurate, sensitive and low-cost method for measuring the amount of the coenzyme Q10 in the biological sample is established, the mobile phase selection of LC-MS / MS detection of the CoQ10 is optimized by virtue of the capability of processing uncertain data through the fuzzy logic, and the precision of the method is remarkably improved compared with that of similar researches.

A wound healing accelerating dressing, its preparation method, and its application belong to the field of functional composite materials. The wound dressing comprises a double-layer structure; the upper layer is an antibacterial adhesive film, prepared by a solution reaction of raw materials including the following components: isophorone diisocyanate, triphenylmethane triisocyanate, hexafluoroisopropanol, polyetheramine 2000, N-methyldiethanolamine, glacial acetic acid, organotin catalyst, and acetone; the lower layer is prepared by a solution reaction of raw materials including the following components: isophorone diisocyanate, polyetheramine 2000, N-methyldiethanolamine, glacial acetic acid, liquid metal, gallic acid, and acetone. This dressing can achieve synergistic effects of protection, antibacterial properties, and electrical stimulation without the need for an external power source, and can be used as a wound healing accelerating dressing that mediates scarless repair, as well as other scar prevention medical materials.

The invention discloses a kit and a method for extracting DNA (deoxyribonucleic acid) in edible oil. The kit comprises a release unit, an adsorption unit and an enrichment unit, the release unit comprises a TE buffer solution, Tween 20, 3-[(3-cholic acid acyloxy) propyl] dimethylamino-1-propanesulfonic acid and sodium chloride; the adsorption unit comprises a PEI nanofiber membrane and guanidine hydrochloride; and the enrichment unit comprises polyethylene glycol 8000 and isopropanol. The DNA can be effectively extracted from the refined edible oil through the kit and the matched extraction method. The kit and the extraction method provided by the invention can provide a reliable DNA template basis for molecular detection technologies such as edible oil molecule traceability, adulteration detection, transgenic screening and the like. Meanwhile, the extraction method is simple and easy to operate, environment-friendly and non-toxic in extraction process, high in extraction efficiency and suitable for standardized popularization and application. Therefore, the kit and the extraction method have good application prospects and industrialization potential.

The invention discloses a preparation method of an absorbable antibacterial coating material capable of slowly releasing antibiotics. The preparation method comprises the following steps: step 1, blending antibiotics, an absorbable polymer and a mixed solvent to obtain a coating solution; the antibiotics are selected from rifampicin and minocycline hydrochloride; the absorbable polymer is selected from one or more of polyglycolide, polylactide, polyglycolide-co-lactide, polyglycolide-co-caprolactone and tyrosine polyarylester; the absorbable polymer is selected from one or more of polyglycolide, polylactide, polyglycolide-co-lactide, polyglycolide-co-caprolactone and tyrosine polyarylester; the mixed solvent is selected from hexafluoroisopropanol and acetonitrile, and the volume ratio of the hexafluoroisopropanol in the mixed solvent is not less than 25%; and 2, uniformly spraying the coating solution on the surface of the degradable base material through ultrasonic spraying, and then carrying out drying treatment. According to the preparation method disclosed by the invention, the antibiotic, the absorbable polymer and the organic solvent are blended and dissolved and then are attached to the surface of the absorbable material through ultrasonic spraying, and the release of the antibiotic is regulated and controlled while the stability of the coating is ensured by regulating and controlling the ratio of the antibiotic to the polymer and the swelling characteristic of the solvent to the base material.

This invention provides a glucose-sensitive and antifouling bifunctional probe, its preparation method, and its application, belonging to the field of glucose detection technology. It addresses the problems of poor sensitivity and antifouling stability in existing biological probes used for glucose detection. The preparation method of the glucose-sensitive and antifouling bifunctional probe includes: preparing a phenylboronic acid hydrogel membrane as a glucose-sensitive functionalized probe; mixing 2-bromoisobutyric acid, 3,4-bis(tert-butyl-dimethyl-siloxy)-1-phenylalanine, and a chemical bridge containing a primary amine group, and performing an amidation reaction to obtain an ATRP initiator; mixing an antifouling monomer and the ATRP initiator, and performing an ATRP reaction to obtain an antifouling coating solution; wherein the chemical bridge containing the primary amine group is 1,3-diamino-isopropanol and / or pentaerythritol; and spin-coating the antifouling coating solution onto the surface of the phenylboronic acid hydrogel membrane of the glucose-sensitive functionalized probe to obtain a glucose-sensitive and antifouling bifunctional probe with an antifouling coating. This glucose-sensitive and antifouling bifunctional probe exhibits high sensitivity and antifouling stability.

The application discloses a preparation method of a dual-function antibacterial and anti-inflammatory absorbable coating material, which comprises the following steps: (1) blending rifampicin, artemisinin and an absorbable polymer A with a mixed solvent to obtain coating solution A; blending minocycline hydrochloride, aspirin and an absorbable polymer B with the mixed solvent to obtain coating solution B; the mixed solvent is selected from hexafluoroisopropanol and ethyl acetate; (2) uniformly spraying the coating solution A and the coating solution B on the surface of a degradable substrate by ultrasonic spraying, and performing drying treatment to obtain the dual-function antibacterial and anti-inflammatory absorbable coating material. According to the preparation method, the proportion of antibiotic active ingredients, anti-inflammatory drug active ingredients and absorbable polymers is regulated, the antibacterial and anti-inflammatory drug coating is uniformly and stably fixed on the surface of the degradable substrate by ultrasonic spraying, and the dual-function absorbable antibacterial and anti-inflammatory coating material with controllable release is prepared by optimizing process parameters.

The invention discloses a preparation method of a degradable antibacterial material with slow release performance. The preparation method comprises the following steps: step 1, blending antibiotics and a mixed solvent to obtain an antibiotic solution; the antibiotic is selected from rifampicin and minocycline; the mixed solvent is selected from hexafluoroisopropanol and acetonitrile, and the volume ratio of the hexafluoroisopropanol in the mixed solvent is not less than 25%; step 2, uniformly spraying the antibiotic solution on the surface of a degradable base material through ultrasonic spraying, and then drying to obtain the degradable antibacterial material with the slow release performance. According to the preparation method disclosed by the invention, by regulating and controlling the composition of the mixed solvent and ultrasonic spraying parameters and utilizing the controllable swelling effect of the mixed solvent on the degradable base material, antibiotic molecules are embedded into the surface layer of the material in the atomization spraying process, so that the interface bonding force between the medicine and the degradable base material is remarkably enhanced, and the burst release behavior is effectively inhibited; the continuous and stable in-vitro release performance is realized.

This invention discloses a kit, method, and application for extracting Helicobacter pylori nucleic acid from fecal samples. The kit includes: sample processing solution: guanidine isothiocyanate, lithium dodecyl sulfate; lysis buffer: magnetic beads, guanidine isothiocyanate, lauramide propyl hydroxysulfonate betaine, potassium chloride, tris(hydroxymethyl)aminomethane, isopropanol; washing buffer I: guanidine hydrochloride, Tween 20, tris(hydroxymethyl)aminomethane; washing buffer II: betaine, guaiacol, ethanol; washing buffer III: ethanol; elution buffer: tris(hydroxymethyl)aminomethane. Using this kit for extracting Helicobacter pylori nucleic acid from fecal samples can fully digest impurities, reduce the adsorption of impurities to magnetic beads, reduce the cross-linking of impurities and nucleic acids, increase the effective adsorption of nucleic acids to magnetic beads, and improve the yield and quality of Helicobacter pylori nucleic acid in fecal samples.

The invention provides a composite gel for biological tissue in-situ derivatization and mass spectrum imaging application. The composite gel comprises a gel skeleton matrix, an organic solvent and a derivatization reagent, the derivatization reagent and the organic solvent are dispersed inside and on the surface of the three-dimensional network structure of the composite gel; the organic solvent comprises at least one of methanol, acetone, acetonitrile, isopropanol and dimethyl sulfoxide; the derivatization reagent is prepared from at least one of acetone, 2-acetylpyridine, benzophenone, 2-benzoylpyridine, 2, 4, 6-trifluoroacetophenone and methyl benzoylformate, and the derivatization reagent is prepared from at least one of acetone, 2-acetylpyridine, benzophenone, 2-benzoylpyridine, 2, 4, 6-trifluoroacetophenone and methyl benzoylformate. According to the composite gel, a non-diffusible solution-state micro-reaction environment is constructed, the bearing capacity of various derivatization reagents is improved by introducing an organic reagent, and the in-situ derivatization efficiency of a sample is improved. Therefore, by adopting the composite gel disclosed by the invention, efficient in-situ derivatization can be realized on the surface of a biological sample, the detection sensitivity of mass spectrum imaging of the biological sample is improved, and the spatial distribution of a lipid fine structure is accurately identified.