123 results about "Isoprodian" patented technology

The invention discloses a method for simultaneously extracting DNA (deoxyribonucleic acid) and RNA from lily tissue. The method comprises the following steps of: obtaining total nucleic acid solution through crude extraction and purification of the same steps from a lily tissue sample; and then sequentially selectively precipitating RNA and DNA, thereby obtaining high-quality DNA and RNA. The method disclosed by the invention is short in time, economic and fast, and good in stability; and the extracted nucleic acid is high in quality. The method has the characteristics that high-concentration potassium acetate is utilized for precipitating polysaccharides twice, so that the polysaccharides in the lily sample can be effectively removed; and in the DNA and RNA separation process, the RNA is selectively precipitated by means of the synergistic effect of lithium chloride and absolute ethyl alcohol and then the DNA is precipitated by using sodium acetate and isopropanol; and therefore, the efficiency of the DNA-RNA separation is high, the loss of the nucleic acid is low and the precipitation time is greatly shortened. The method is suitable for simultaneously extracting the DNA and the RNA from the lily tissue containing rich polysaccharides and other secondary metabolites.

A blood cell analyzer diluent and a hemolytic agent, are characterized in that one litre diluent is provided with 12.0-4.0g of sodium chloride, 2.0-10.0g of sodium sulfate, 0.8-0.5g of 1,3,2-methylol urea, 0.2-0.5g of copper sulfite, 3.0-8.0g of EDTA-2Na, 0.2-0.7g of Piperacillin Sodium, a borate buffering liquid toning the ph value to 7.2-7.8, and the balance is water; one litre hemolytic agent is provided with 0.8-5.0g of potassium chloride, 0-60.0g of dodecyl trimethyl ammonium chloride, 14.0-0g of octadecyl trimethyl ammonium bromide, 6.0-10.0ml of isopropanol, carbonate or alcaine buffering liquid toning the ph value to 7.2-7.8, and the balance is water. The inventive reagent can form stable hemoglobin derivatives, and the absorption spectrum curves are similar when lambada is 540nm, lambada is 504nm, which can satisfy the clinical inspection requirement; the reagent does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operating staff, and can reduce harm of toxicant to personal health.

The invention discloses chitosan ester p-aminobenzoate, which has a structure shown in the specifications, wherein X is between 0.65 and 0.95, and the molecular weight is between 70 and 80 kilodaltons. The materialization indexes of the compound are that: the chitosan ester p-aminobenzoate is a pale yellow powder solid, can be dissolved in water and is dissolved in ethanol and isopropanol slightly. After a p-animo-benzoyl structure is introduced into a chitosan molecular chain, the minimum inhibitory concentration of the compound on staphylococcus aureus and aspergillus niger is 0.1 percent and 0.25 percent respectively, so the chitosan ester p-aminobenzoate can be used as food antiseptic additives.

The invention provides an extraction method of total DNA of metagenome of fish enteric microorganisms and a kit. The extraction method comprises the following steps: (1) fish sample sampling and fixing treatment of microorganisms on the surface of the fish; (2) acquisition of enteric microorganisms and collection of thalli; (3) embedding and cracking of cells; (4) extraction of DNA; and (5) DNA preservation. The kit comprises the following main components: a solution A which is a mixed solution of Tris and EDTA; a solution B which is a mixed solution of Tris, EDTA, NaCL, PVP, guanidinium isothiocyanate, Triton-X100, PMSF, and beta-mercaptoethanol; a solution C which is a mixed solution of Tris, EDTA, NaCl and DTT; a solution D which is a mixed solution of proteinaseK, a lysozyme, Tris, NaCl and SDS; a solution E which is a mixed solution of Tris saturated phenol and chloroform; F which is isopropanol; and G which is an ethanol aqueous solution. The DNA fragment obtained by the invention is high in purity, and the content of DNA of a host and the microorganisms on the surface of the fish is small.

The invention discloses a method for extracting and separating total proteins of mice sperms by using two-dimensional electrophoresis. The method comprises the following steps: preparing sperms, precipitating DNAs of the sperms after lysis of the sperms, preparing sperm proteins, preparing a protein extraction sample, carrying out first-dimensional isoelectric focusing electrophoresis, carrying out second-dimensional SDS-polyacrylamide gel electrophoresis, dyeing by using coomassie brilliant blue, preparing gel and scanning by using an image scanner. According to the method, lysis of the sperms is completely performed by Trizol, the proteins are extracted by chloroform, impurities are removed by isopropanol, guanidine hydrochloride and ethyl alcohol, and the proteins are purified by acetone, so that the problems of low content of the mice sperms, collection difficulty, small cell volume, a small amount of cytoplasm and a large amount of acrosomal protease are well solved; the sample prepared by the method is complete to prepare, has a large quantity of protein isolating points, and is high in repeatability and clear in chromatogram; by virtue of detection of PDQuestsoftware, the number of the protein points is greater than 1,000; therefore, the method lays the foundation for further mass spectrometry.

The invention provides a total DNA (deoxyribonucleic acid) extraction method and a kit for synchronously removing humic acid and mycoprotein and relates to the field of research of metagenomics in soil microbiology and general microbiology. The method comprises the following steps: (1) suspending samples by utilizing a humic acid combination solution, so as to obtain sediments; (2) adding a buffer solution and proteinase K, and carrying out centrifuging to obtain supernate; (3) adding a coagulant to carry out coagulating sedimentation on the residual humic acid and mycoprotein, and carrying out centrifuging to obtain the supernate; (4) adding isopropanol to precipitate DNA; (5) adding 70% ethanol to wash the sediments, and blowing off the residual ethanol; (6) adding a DNA dissolving solution (TE) to dissolve the sediments, so as to obtain a sample, namely the total DNA. The kit comprises the following components: the humic acid combination solution, a PBS butter liquid, an extraction butter liquid, the proteinase K, a 20% SDS (sodium dodecyl sulfonate) water solution, the coagulant, a DNA washing agent, the 70% ethanol and the DNA dissolving solution. Due to the adoption of the method and the kit, the one-time extraction quantity of the total DNA is enough to carry out PCR (polymerase chain reaction) amplification for dozens of times.

The invention discloses a method for preparing a cell matrix-free material for a porcine adipose tissue. The method comprises the following steps: (1) taking fresh porcine subcutaneous fats, cleaning, cutting into pieces, adding water or normal saline, homogenating, and centrifuging to obtain a precipitate, and rinsing by PBS solution or water; (2) crushing the precipitate, rinsing by the SDS solution, then rinsing by the PBS solution or water, and centrifuging to obtain a precipitate; and (3) rinsing the precipitate by isopropanol solution, and then rinsing by the PBS solution or water to obtain the product. The acellular matrix material prepared by the method disclosed by the invention is thorough in decellularization, low in immunogenicity, good in biocompatibility, simple in preparation method, low in cost and good in application prospect.

The invention aims to provide a chlorhexidine gluconate composite disinfectant. The chlorhexidine gluconate composite disinfectant is prepared from chlorhexidine gluconate, coco fatty acid diethanol amide, lemon essence, delta-gluconolactone, hydroxyethyl cellulose, isopropanol, lauryl amine oxide, wool grease, tridecanol, and water for injection. The chlorhexidine gluconate composite disinfectant can perform comprehensive disinfection treatment on infection of skin, wound, trauma and burn before and after a surgery, can comprehensively disinfect the surgical instruments, has wide bacteriostatic and bactericidal effects and high effects, has a killing effect on all gram-positive bacterium, gram-negative bacterium, fungus, acid-fast bacteria, germs and viruses, is still effective in presence of serum and blood, causes no irritation to skin tissues, and is convenient and safe to use.

The invention discloses a kit for extracting blood free DNA. The kit of the invention includes a lysis solution, a binding solution, a cleaning solution 1 and a cleaning solution 2. The binding solution contains 2-5 M guanidine isothiocyanate or guanidine hydrochloride, 10-100mM Tris-HC1, 10-50mM EDTA, 5-15% Triton X-100, and 20-50% absolute ethanol or isopropanol. The cleaning solution 1 contains2-5 M guanidine isothiocyanate or guanidine hydrochloride, 10-100mM Tris-HCl, 10-50mM EDTA, 5-10% Triton X-100, SDS or Tween 20, and 20-50% absolute ethanol or isopropanol. The kit of the invention improves the binding solution and increases the extraction efficiency, improves the cleaning solution 1, increases the binding efficiency of the magnetic beads cfDNA and removes impurities, improves the recovery efficiency and purity of the cfDNA and reduces the material cost.

The invention belongs to the technical field of biological medicines, and discloses a betulinic acid derivative, a preparation method and application thereof. The preparation method comprises the following steps: preparing biotin esterification coupled oligomerization ethylene glycol carboxylic acid or biotin amide coupled oligomerization ethylene glycol carboxylic acid; dissolving betulinic acidin a solvent, and carrying out a stirring reaction at 0 DEG C for 1-6 hours under the action of a dehydrating agent and a catalyst; adding biotin or the biotin esterification coupled oligomerization ethylene glycol carboxylic acid or biotin amide coupled oligomerization ethylene glycol carboxylic acid according to a betulinic acid molar ratio of 1:1-3, heating to room temperature from an ice bath,and stirring in a dark place overnight; and concentrating the filtrate, re-crystallizing with ice diethyl ether or isopropanol, carrying out chromatography or preparative liquid phase purification, and freeze-drying to obtain the betulinic acid derivative. The betulinic acid derivative, the pharmaceutically acceptable salt and the isotope marker thereof can be applied to preparation of anti-cancer drugs and drugs for treating obesity or non-alcoholic fatty liver disease.

The invention relates to a method for determining peramivir intermediate isomers by using high performance liquid chromatography. The method is characterized in that an adopted chromatographic columnis a polysaccharide derivative coated chiral chromatographic column; the adopted mobile phase is a mixed solution of isopropanol and normal hexane, and isocratic elution is adopted in a high performance liquid chromatography system; in the mobile phase, the volume ratio of isopropanol to normal hexane is 10: 90 to 20: 80; the flow velocity of the mobile phase is 0.8 to 1.0 ml/min; an adopted detector is an ultraviolet detector, and the monitoring wavelength is 215 nm. The method overcomes the defects in the prior art, solves the problem of analytical determination of the peramivir intermediateisomer, can effectively control the contents of the target product and isomer impurities thereof, avoids the interference of the isomer impurities on the subsequent synthesis reaction, enhances the quality of the subsequent prepared peramivir, and ensures the medication safety. The invention provides an accurate and efficient detection method for determining the isomer of the peramivir intermediate.

The invention provides a valve material with synergistic anticoagulation and anti-calcification functions and a preparation method of the valve material. The preparation method comprises the followingsteps: carrying out glutaraldehyde crosslinking treatment on an animal-derived biological valve material; immersing the treated valve material in confining liquid containing an amino compound to perform treatment for 0.5-6 h, and then confining aldehyde groups remaining after glutaraldehyde crosslinking; then putting the valve material into a reaction solution containing an anticoagulant and a cross-linking agent, and carrying out cross-linking treatment for 6-24 hours at 4-37 DEG C; and finally, cleaning the valve material to obtain the valve material, and storing the valve material in a glutaraldehyde or isopropanol/glycerol mixed solvent. The method can effectively solve the problems of easy calcification and thrombus formation caused by aldehyde group residue in the valve material prepared by an existing method, and the valve material prepared by the method disclosed by the invention can be used as a valve material required by aortic valve, pulmonary valve, venous valve, mitral valve and tricuspid valve replacement.

The invention relates to a novel coronavirus COVID-19 marker detector and a detection method. The detector comprises a laser emitting device, a light angle adjusting device, a beam expander, a gas receiving device, a signal processing device and a PC processing terminal, wherein the gas receiving device is internally provided with a triangular annular ring-down cavity, a temperature controller anda pressure gauge; laser emitted by the laser emitting device vertically enters the beam expander to be processed after passing through the light angle adjusting device, then enters the triangular annular ring-down cavity of the gas receiving device to be measured and is transmitted to the PC processing terminal through the signal processing device. According to the detection method, whether a patient with novel coronal pneumonia is diagnosed by detecting whether the concentrations of ethyl butyrate, butyraldehyde, isopropanol and acetone in respiratory gas are abnormal or not. The optical cavity ring-down spectroscopy technology has the advantages of being high in precision, short in time, low in detection cost, capable of greatly improving the medical diagnosis efficiency, good in economic space, large in research and development value and considerable in development space.

The invention discloses a preparation method of extracellular matrix-imitated printable composite biological ink, which comprises the following steps of: (A) dissolving GelMA powder and a photoinitiator in PBS, and uniformly stirring at 40-50 DEG C to prepare a GelMA solution with the final concentration of 5-15%; (B) pretreating fresh adipose tissues, mashing the pretreated adipose tissues, respectively treating the mashed adipose tissues by 0.25% trypsin-0.1% EDTA (Ethylene Diamine Tetraacetic Acid) and 3% Triton-X100 for 1 hour, and respectively treating the treated adipose tissues by 4% sodium deoxycholate and 100% isopropanol; (C) dissolving the acellular extracellular matrix obtained in the step (B) into 0.01 M HCL and 1mg/mL pepsin solution, stirring at 4-10 DEG C, and centrifugingat a low speed for 10min to obtain a deadipose extracellular matrix solution with the final concentration of 5-15mg/mL; and (D) preheating the GelMA prepared in the step (A) at a proper temperature, slowly adding the solution in the step (C) at a vortex oscillation speed of 1500-2500rpm according to a volume ratio of the GelMA to the acellular extracellular matrix of (1-4): 1, adjusting the pH value, and performing sterilizing. The composite biological ink prepared by the method has excellent printability, biocompatibility and biological activity.

The present invention relates to an one-step method ribonucleic acid extraction reagent EZRNA. Its formula includes (by 1 L total amount) guanidinium isothiocyanate 3-5 M, sodium citrate 18-25 M, sodium laurate (by wt%) 0.3-0.6%, 2-mercaptoethanol 0.6-1.2 M and balanced acidic phenol (by volume%) 40-55%. Besides, said invention also provides the concrete steps of the invented one-step method ribonucleic acid extraction process by using said reagent.

The invention belongs to the technical field of high polymer materials and relates to a polyaspartic acid type ice point regulator preparation method by taking polyaspartic acid and polyaspartic acid derivatives as the raw materials. A macromolecular polyaspartic acid type grafted copolymer is provided, and is synthesized by a midbody polysuccinimide of polyaspartic acid amino acid and a graft, wherein the graft comprises alcohol amine type substances, such as ethanol amine, isopropanolamine, diglycolamine, diethanol amine, 2-amino-1-butanol, N-(3-aminopropyl)diethanol amine, 3-amino propanol, 2-(methylamino)ethanol, D-phenylglycinol, diisopropanol amine and N-(2-ethoxy)ethylenediamine. The copolymer can be taken as an environment-friendly macromolecular ice point regulator, and has high application value in the energy saving field of a dynamic ice-slurry cold-storage air-conditioning system and the field of food additives and medical fields.

The invention relates to a phosphodiesterase 4 inhibitor apremilast composition and a quality detection method. The apremilast composition is a tablet, comprises lactose, magnesium stearate and otherauxiliary materials, and is used for treating adult patients suffering from active psoriatic arthritis, adult patients suffering from moderate-to-severe plaque psoriasis and suitable for phototherapyor systemic therapy, and adult oral ulcer patients related to Bessel's disease. The quality detection method comprises the step of determining the content of the enantiomer in the tablet, and comprises the following operations: by using a chiral chromatographic column and using n-hexane, isopropanol and the like as mobile phases, in a high performance liquid chromatography system, injecting 5 [mu]l of a system applicable solution into a liquid chromatograph, wherein the peak appearance sequence is enantiomer peaks and apremilast peaks successively, and the separation degree between two peaks is not less than 1.5, and precisely measuring 5 [mu]l of the test solution, injecting the test solution into a liquid chromatograph, recording a chromatogram, and calculating the content of the enantiomer according to an area normalization method if enantiomer peaks exist in the chromatogram of the test solution. The compositions and methods of the present invention exhibit excellent technical effects as described in the specification.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly provides a method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri, which comprises the following steps: (1) preparing a test solution, a reference solution and a reference medicinal material solution; (2) thin layer chromatography detection: carrying out two-time expansion and respective inspection, wherein butyl acetate-methanol-isopropanol-water is adopted as a developing solvent for first-time developing, n-butanol-water-acetic acid is adopted as a developing solvent for second-time developing, a'secondary thin-layer developing method ' is utilized for the first time, the developing solvent with specific composition is adopted, the same thin-layer plate is adopted, and second-time sample application is not needed; the method can be used for simultaneously and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, avoids the increase of resources and cost caused by independently and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, ensures the uniformity, stability and reliability of quality, and has the advantages of high accuracy, time and cost saving and easiness in popularization and application.