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Method for distinguishing production places of cordyceps sinensises based on stable isotope ratio

A technology of isotope ratio and Cordyceps sinensis, which is applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems that are not conducive to the healthy development and orderly competition of the Cordyceps sinensis industry, damage the interests of consumers and the right to know, and cannot effectively regulate the market, etc. problems, to achieve good application prospects, stable and reliable methods, and the effect of promoting healthy development

Inactive Publication Date: 2014-03-19
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, my country still lacks the technical means and methods to effectively identify the origin of Cordyceps sinensis. Only relying on the self-discipline of enterprises and the existing standard management system cannot effectively regulate the market. Driven by interests, very few enterprises use fake and inferior Cordyceps sinensis to pretend to be high-quality origin Cordyceps sinensis and obtain high profits. Profits will have a great impact on the Cordyceps sinensis market, which is not conducive to the healthy development and orderly competition of my country's Cordyceps sinensis industry, and at the same time damages the interests of consumers and the right to know

Method used

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  • Method for distinguishing production places of cordyceps sinensises based on stable isotope ratio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: δ of Cordyceps sinensis samples from the real place of origin 15 N determination

[0018] 1.1 Collection of real origin samples

[0019] Samples of Cordyceps sinensis were collected from 12 different production areas in Qinghai, Sichuan, Yunnan and Nepal, and 10 samples from different meadows were collected in each production area, as shown in Table 1 below.

[0020] Table 1 Collection list of real origin samples

[0021] serial number Sample serial number sample name Sample source 1 YSHX Cordyceps sinensis Haxiu, Yushu Prefecture, Qinghai 2 QUR Cordyceps sinensis Guo Qin, Yushu Prefecture, Qinghai 3 YSYS Cordyceps sinensis Yushu County, Yushu Prefecture, Qinghai 4 YZD Cordyceps sinensis Qinghai Yushu Zhiduo County 5 QHGL Cordyceps sinensis Qinghai Guoluo Prefecture 6 QHH Cordyceps sinensis Qinghai Hainan Prefecture 7 SCAB Cordyceps sinensis Aba Prefecture, Sichuan 8 ...

Embodiment 2

[0033] Embodiment two: the identification example of the origin of Cordyceps sinensis

[0034] 2.1 Source of samples

[0035] Three samples of Cordyceps sinensis marked as Qinghai, Sichuan and Yunnan were purchased from the market.

[0036] 2.2 Nitrogen isotope determination of samples

[0037] 1) Take one sample of different Cordyceps sinensis, and grind it into a fine powder with a tissue grinder (Beijing Ecosines Co., Ltd.) at 1600 rpm for 5 min.

[0038] 2) Take 0.2g of sample powder respectively, and convert it into pure N by Flash EA1112 elemental analyzer 2 gas. The working parameters of the elemental analyzer are: the injector helium purge flow rate is 200ml min -1 , the temperature of the oxidation furnace is 980 °C, the temperature of the reduction furnace is 650 °C, and the flow rate of the carrier gas helium is 90 ml min -1 .

[0039] 3) Generated N 2 The gas was diluted by a Conflo III diluter, and the conditions were set: the helium dilution pressure was 0...

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Abstract

The invention discloses a method for distinguishing production places of cordyceps sinensises based on stable isotope ratio, and belongs to the field of a cordyceps sinensis authenticity distinguishing technology. The method comprises the following main steps: (1), detecting the delta 15N value of a cordyceps sinensis sample of a real production place with a stable isotope mass-spectrometric technique, constructing a delta 15N database of the cordyceps sinensis sample of the real production place; (2), detecting the delta 15N value of a to-be-detected cordyceps sinensis sample; (3) performing significant difference analysis on the delta 15N value of the to-be-detected sample and the delta 15N value of the cordyceps sinensis sample of the real production place in the database, indicating that the to-be-detected sample and the real sample are from the same production place if the significant difference is inexistent, and otherwise, indicating that the to-be-detected sample and the real sample are from different production places. A method capable of exactly distinguishing production places of cordyceps sinensises is provided by using the stable isotope mass-spectrometric technique, consequently, the technical problems in market supervision of the production places of cordyceps sinensises in China is solved, the method is beneficial for standardizing market behavior, protecting legal interests of consumers and enterprises, and improving the cordyceps sinensis industries to develop in a healthy and ordered way.

Description

technical field [0001] The invention belongs to the field of medicinal material identification, and in particular relates to a method for identifying the origin of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a rare species resource in the Qinghai-Tibet Plateau region of my country. It has health functions such as improving human immunity and anti-tumor and has special medicinal effects. Its effective effect has been widely recognized by the people. [0003] The origin of Cordyceps sinensis is an important indicator to measure its value. Cordyceps sinensis from different origins has obvious differences in nutritional components and functions, as well as differences in value. However, my country still lacks the technical means and methods to effectively identify the origin of Cordyceps sinensis. Only relying on the self-discipline of enterprises and the existing standard management system cannot effectively regulate the market. Driven by interests, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 程池扎西才吉李辉姚粟刘洋白飞荣钟其顶
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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