Method for producing neomycin by replacing part of fermenting raw materials with soybean meal

A technology for replacing part and fermenting raw materials, applied in the biological field, can solve the problems of high cost and complex raw material formula of culture medium, and achieve the effects of alleviating the pressure of environmental protection, being beneficial to warehouse management and saving costs.

Active Publication Date: 2014-03-26
YICHANG SANXIA PHARMA
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for producing neomycin by substituting soybean flour for part of the fermented raw materials, which can solve the problem of complex raw material formula and high cost of the culture solution, effectively simplifies the formula, reduces the cost, and guarantees the production of neomycin. production of mycin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing neomycin by replacing part of fermenting raw materials with soybean meal
  • Method for producing neomycin by replacing part of fermenting raw materials with soybean meal
  • Method for producing neomycin by replacing part of fermenting raw materials with soybean meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for producing neomycin by substituting soybean flour for part of fermented raw materials, the method consists of the following steps:

[0035] 1) Cultivation of inclined spores: the sand spores are sealed, dried, and stored at 0-4°C to obtain inclined spores;

[0036] 2) Cultivate the slant spore suspension: culture the slant spore at 28±2°C for 4 to 12 days to prepare the slant spore suspension;

[0037] 3) Seed tank culture: Put the slant spore suspension into the seed culture tank for cultivation. The culture solution in the seed tank includes soybean powder with a mass content of 2.0% of the mass of the culture solution in the fermenter, and the culture solution in the seed tank does not contain peptone, yeast powder and peanut powder;

[0038] 4) Fermentation tank culture: send the culture liquid cultivated in the seed tank into the fermentation culture tank for cultivation. The culture liquid in the fermenter includes soybean powder with a mass content o...

Embodiment 2

[0049] A method for producing neomycin by substituting soybean flour for part of fermented raw materials, the method consists of the following steps:

[0050] 1) Cultivation of inclined spores: the sand spores are sealed, dried, and stored at 0-4°C to obtain inclined spores;

[0051] 2) Cultivate the slant spore suspension: culture the slant spore at 28±2°C for 4 to 12 days to prepare the slant spore suspension;

[0052] 3) Seed tank culture: Put the slant spore suspension into the seed culture tank for cultivation. The culture solution in the seed tank includes soybean powder with a mass content of 5.0% of the mass of the culture solution in the fermenter, and the culture solution in the seed tank does not contain peptone, yeast powder and peanut powder;

[0053] 4) Fermentation tank culture: send the culture liquid cultivated in the seed tank into the fermentation culture tank for cultivation. The culture liquid in the fermenter includes soybean powder with a mass content o...

Embodiment 3

[0064] A method for producing neomycin by substituting soybean flour for part of fermented raw materials, the method consists of the following steps:

[0065] 1) Cultivation of inclined spores: the sand spores are sealed, dried, and stored at 0-4°C to obtain inclined spores;

[0066] 2) Cultivate the slant spore suspension: culture the slant spore at 28±2°C for 4 to 12 days to prepare the slant spore suspension;

[0067] 3) Seed tank culture: Put the slant spore suspension into the seed culture tank for cultivation. The culture solution in the seed tank includes soybean powder with a mass content of 3.0% of the mass of the culture solution in the fermenter, and the culture solution in the seed tank does not contain peptone, yeast powder and peanut powder;

[0068] 4) Fermentation tank culture: send the culture liquid cultivated in the seed tank into the fermentation culture tank for cultivation. The culture liquid in the fermenter includes soybean powder with a mass content o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for producing neomycin by replacing part of fermenting raw materials with soybean meal. The method consists of the following steps: (1) culturing bevel spores; (2) culturing a bevel spore suspension; (3) performing seeding tank culturing: putting the bevel spore suspension into a seed culture tank for culturing; (4) performing fermentation tank culturing: feeding a culture solution subjected to the seed tank culturing into a fermentation culture tank for culturing, wherein the culture solutions in the seed tank and the fermentation tank include soybean meal of which the mass content is 2.0-5.0 percent the mass of the culture solution in the fermentation tank, and the culture solution in the fermentation tank does not contain peptone, yeast powder and peanut powder; (5) terminating fermentation, diluting a fermentation solution, neutralizing till the pH value becomes 6.4-7.0 to obtain a diluted fermentation solution, thereby finishing the fermentation step. According to the method for producing the neomycin by replacing part of the fermenting raw materials with the soybean meal, the problems of complex raw material formula and high cost of the culture solution are solved, the formula is simplified effectively, the cost is reduced, and the production of the neomycin is guaranteed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing neomycin by substituting soybean flour for part of fermented raw materials. Background technique [0002] The raw materials of the culture medium used for the fermentation of neomycin in China are mainly grain processing raw materials, especially in a wide variety of organic nitrogen sources, including peanut powder, soybean powder, yeast powder, peptone, corn steep liquor and other ingredients. [0003] However, the variety of raw materials will bring a series of problems, such as the fluctuation of fermentation production caused by the unstable quality of raw materials; the difficulty of storage and transportation caused by the complexity of distribution materials; The price of the product is relatively expensive, so that the production cost of the product remains high, and it is at a disadvantage in the fierce market competition. Contents of the invention ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/52
Inventor 沈联兵姚明高郑兴
Owner YICHANG SANXIA PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap