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Kit and method for determining Lambda free light chain concentration

A technology of free light chains and kits, applied in the biological field, to achieve high throughput, facilitate production amplification and popularization and application, and fast learning speed

Active Publication Date: 2015-07-15
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a test kit and method for measuring the concentration of Lambda free light chains in order to overcome the defects of the existing Lambda free light chain assay non-specific reaction

Method used

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  • Kit and method for determining Lambda free light chain concentration
  • Kit and method for determining Lambda free light chain concentration
  • Kit and method for determining Lambda free light chain concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Improve kit A, change the concentration of Tween-20 in the Lambda free light chain antibody solution (R2 reagent) to 11ml / L, keep the concentration of other components unchanged, and record it as kit A-a, the specific composition is as follows:

[0043] Composition of the reaction buffer (R1 reagent) in the A-a kit

[0044] Element

name

concentration

1

4-Hydroxyethylpiperazineethanesulfonic acid (Hepes)

100mmol / L

2

Sodium chloride (NaCl)

150mmol / L

3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0045] Composition of Lambda free light chain antibody solution (R2 reagent) in A-a kit

[0046] Element

name

concentration

1

Lambda Free Light Chain Antibody

0.5mg / ml

2

latex microspheres

3mg / ml

3

Tween-20

11ml / L

4

Preservative Proclin-300

0.3ml / L

[0047] Drawing of working curve:

[0048] Using the lat...

Embodiment 2

[0056] Improve kit A, change the concentration of Tween-20 in the Lambda free light chain antibody solution (R2 reagent) to 16ml / L, keep the concentration of other components unchanged, and record it as kit A-b. The specific composition is as follows:

[0057] Composition of reaction buffer (R1 reagent) in A-b kit

[0058] Element

name

concentration

1

4-Hydroxyethylpiperazineethanesulfonic acid (Hepes)

100mmol / L

2

Sodium chloride (NaCl)

150mmol / L

[0059] 3

Tween-20

1ml / L

4

Preservative Proclin-300

0.3ml / L

[0060] Composition of Lambda free light chain antibody solution (R2 reagent) in A-b kit

[0061] Element

name

concentration

1

Lambda Free Light Chain Antibody

0.5mg / ml

2

latex microspheres

3mg / ml

3

Tween-20

16ml / L

4

Preservative Proclin-300

0.3ml / L

[0062] Drawing of working curve:

[0063] Us...

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Abstract

The invention discloses a kit and a method for determining Lambda free light chain concentration. The kit comprises a reaction buffer and a Lambda free light chain antibody solution; the Lambda free light chain antibody solution has a Tween-20 concentration of 11-16 ml / L. The kit of the invention solves the problem of nonspecific reaction in Lambda free light chain turbidimetric determination, provides clinical doctors with reliable Lambda free light chain determination results as diagnostic reference, thus gives full play to the advantages of high speed and high flux of latex enhanced immunoturbidimetric method, shortens the detection time, and facilitates clinical application and patient diagnosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a method for measuring the concentration of Lambda free light chains. Background technique [0002] Immunoglobulin (Ig) light chains are divided into two types, kappa (kappa) and lambda (lambda), each Ig molecule has only one type of light chain, and the ratio of human kappa (kappa) and lambda (lambda) is 6:4. Light chains are small molecular proteins that can freely pass through the glomerular basement membrane, and are reabsorbed back into the blood circulation in the renal tubules, so only a small amount of light chains exist in normal human urine. When metabolic disorders and multiple myeloma occur in the human body, a large number of free light chains appear in the blood and are excreted in the urine, which is Bence-Jones protein (protein of the week). In a normal human body, the serum κ-type free light chain is 3-19 mg / L, the λ-type free light chain is 6-26 mg / L, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 王金凤蒋欣
Owner BYRON DIAGNOSTICS SHANGHAI
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