Efficient and rapid reproduction method of dendrobium candidum
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A high-efficiency technology of Dendrobium officinale, which is applied in the field of efficient and rapid propagation of Dendrobium officinale, can solve many problems and achieve the effects of promoting effect, good sterile germination effect and good effect.
Inactive Publication Date: 2014-04-16
SUZHOU PASTORAL AGRI TECH DEV
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Problems solved by technology
[0003] In recent years, researchers have conducted a lot of research on the tissue culture rapid propagation technology of Dendrobium candidum, but they mainly focus on...
Method used
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Embodiment 1
[0013] The efficient rapid propagation method of the Dendrobium officinale of the present embodiment may further comprise the steps:
[0014] (1) Adventitious bud induction: Take the young stems of Dendrobium candidum in the same year, cut them into small stems with a length of 1-1.5cm and 1-2 axillary buds, soak them in 75% alcohol for 30s after washing, and then Disinfect with 0.1% mercuric chloride for 10 minutes;
[0015] Cultivate the sterilized Dendrobium stem section in the induction medium for 25 days, the induction medium is: 1 / 2MS basic medium, 0.8mg / L naphthaleneacetic acid, 1.2mg / L 6-benzylaminoadenine, 1.5mg / L L gibberellin, 25g / L sucrose;
[0016] (2) Proliferation of adventitious buds: After the induction, cut off the new shoots that grow from the stem segments at the internodes, inoculate them in the induction medium for subculture and proliferate for 30 days, and obtain clustered buds at the cuts;
[0017] (3) Strong seedlings and rooting transplanting: cut ...
Embodiment 2
[0020] The efficient rapid propagation method of the Dendrobium officinale of the present embodiment may further comprise the steps:
[0021] (1) Adventitious bud induction: Take the young stems of Dendrobium candidum in the same year, cut them into small stems with a length of 1-1.5cm and 1-2 axillary buds, soak them in 75% alcohol for 30s after washing, and then Disinfect with 0.1% mercuric chloride for 10 minutes, and then rinse with sterile water for 5 times;
[0022] Cultivate the sterilized Dendrobium stem section in the induction medium for 28 days. The induction medium is: 1 / 2 MS basic medium, 0.5 mg / L naphthalene acetic acid, 1.3 mg / L 6-benzylaminoadenine, 1.6 mg / L L gibberellin, 30g / L sucrose;
[0023] (2) Proliferation of adventitious buds: After the induction, cut off the new shoots that grow from the stem segments at the internodes, inoculate them in the induction medium for subculture and proliferate for 30 days, and obtain clustered buds at the cuts;
[0024] ...
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Abstract
The invention relates to an efficient and rapid reproduction method of dendrobium candidum. The method comprises the steps as follows: (1), adventitious bud induction: disinfected dendrobium stems are cultivated in an induction medium for 28-30 d, and the induction medium comprises an MS minimal medium, 0.1-0.15 mg/L of naphthylacetic acid, 0.5-1.0 mg/L of 6-benzylaminopurine, 3% of cane sugar and 0.8% of agar; (2), adventitious bud multiplication: after induction is finished, sprouts grown in internodes of the stems are cut off and inoculated in the induction medium for subculture multiplication for 30-32 d, and cluster buds are obtained from cut positions; and (3), strong seedling and root transplanting: the cluster buds are cut into single seedlings and transferred into a strong seedling medium for strong seedling culture for 23-25 d, when the buds develop to the height of 2-3 cm and each bud has 2-3 blades, rooting culture can be performed for 30-32 d, wherein the strong seedling medium comprises the MS minimal medium, 3% of cane sugar and 0.7% of agar, and the rooting medium comprises 1/2 MS minimal medium, 0.3-0.5 mg/L of naphthylacetic acid and 0.3%-0.5% of activated carbon. The plant height of the dendrobium candidum cluster bud cultivated with the method can be up to above 3.9 cm, the number of roots is more than 4, and each root is longer than 1.5 cm.
Description
technical field [0001] The invention relates to the cultivation technology of dendrobium candidum, in particular to a high-efficiency and rapid propagation method of dendrobium candidum. Background technique [0002] Dendrobium candidum, also known as black knot grass, commonly known as Tiepi Fengdou, is a perennial evergreen herb of the genus Dendrobium orchidaceae, and is a precious medicinal material. In recent years, studies have found that Dendrobium contains polysaccharides, alkaloids, phenanthrene and erianin and other compounds, as well as 7 kinds of essential amino acids and various trace elements. Modern pharmacology shows that Dendrobium has anti-tumor, anti-aging, and immune-enhancing functions, and has obvious therapeutic effects on cardiovascular, digestive system, respiratory system and ophthalmic diseases. Due to the medicinal value of Dendrobium candidum with many unique curative effects, it is called "Jiancao of life-saving" among the people, and "the gian...
Claims
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