Method for breeding restoring line or conventional variety by use of cytoplasmic male sterile line of hibiscus cannabinus
A technology of male sterile lines and male sterility, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of limited number of parents, heavy workload, long breeding cycle, etc., and save manual emasculation Effects of the hybridization process, increased productivity levels, and shortened breeding years
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Embodiment 1
[0027] Example 1: Breeding of restorer lines HR1 and HR2
[0028] The kenaf varieties used in this embodiment are known public varieties, as follows:
[0029] K03A: Gui Ke Jian Zi No. [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, see invention patent ZL200510019454.4 (public number CN100415083C).
[0030] K03B: The male sterility maintaining line of the cytoplasmic male sterile line K03A selected by Mr. Zhou Ruiyang of Guangxi University, see invention patent ZL200510019454.4 (public number CN100415083C).
[0031] Fuhong 992: Selected and bred by Fujian Agriculture and Forestry University, passed the identification of the National Hemp Variety Appraisal Committee in July 2007, and the variety identification number: Guopin Jianma 2007005.
[0032] R1: Based on Zhongzahong 318 bred by the Hemp Research Institute of the Chinese Academy of Agricultural Sciences, it was bred by the inventor through 6 generations of the system, ...
Embodiment 2
[0049] Embodiment 2: Breeding of restorer lines HR3 and HR4
[0050] On the basis of the materials used in Example 1, the added materials are:
[0051] 763A: Guikejianzidi [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, using UG93 as the cytoplasmic donor and Taihong 763 as the nuclear donor, bred by saturated backcrossing. The flowers are purple and the stems are light red.
[0052] 763B: Also known as Taihong 763, it is the reincarnated parent (nucleus donor parent) of 763A.
[0053] (1) In 2009, the cytoplasmic male sterile line 763A of kenaf was used as the female parent, and the cytoplasmic male sterile line 763A was propagated with 763B as the male parent; Kenaf hybrids 763A / Fuhong 992 and 763A / R1 were bred with 992 and R1 as male parents, respectively. Taking 763A as the female parent and 763A / Fuhong 992 and 763A / R1 as the male parent, two permanent segregation populations with a ratio of sterile to fertile plants o...
Embodiment 3
[0059] Embodiment 3: Breeding of restorer lines HR5 and HR6
[0060] On the basis of the materials used in Example 1, the added materials are:
[0061] 722A: Guikejianzidi [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, using UG93 as the cytoplasmic donor and 722 as the nuclear donor, bred by saturated backcrossing, completely Leaves, stems green.
[0062] 722B: also known as 722, a kenaf variety bred by the Cannabis Research Institute of the Chinese Academy of Agricultural Sciences, which is the reincarnation parent (nucleus donor parent) of 722A.
[0063] 917A: Guikejianzi [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, using UG93 as the cytoplasmic donor and 917 as the nuclear donor, bred by saturated backcross, split Leaves, stems light red.
[0064] 917B: also known as 917, a kenaf variety bred by the Hemp Research Institute of the Chinese Academy of Agricultural Sci...
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