Method for breeding restoring line or conventional variety by use of cytoplasmic male sterile line of hibiscus cannabinus

A technology of male sterile lines and male sterility, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of limited number of parents, heavy workload, long breeding cycle, etc., and save manual emasculation Effects of the hybridization process, increased productivity levels, and shortened breeding years

Active Publication Date: 2014-04-23
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it requires artificial emasculation and hybridization, the workload is large; it also requires multiple generations of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Breeding of restorer lines HR1 and HR2

[0028] The kenaf varieties used in this embodiment are known public varieties, as follows:

[0029] K03A: Gui Ke Jian Zi No. [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, see invention patent ZL200510019454.4 (public number CN100415083C).

[0030] K03B: The male sterility maintaining line of the cytoplasmic male sterile line K03A selected by Mr. Zhou Ruiyang of Guangxi University, see invention patent ZL200510019454.4 (public number CN100415083C).

[0031] Fuhong 992: Selected and bred by Fujian Agriculture and Forestry University, passed the identification of the National Hemp Variety Appraisal Committee in July 2007, and the variety identification number: Guopin Jianma 2007005.

[0032] R1: Based on Zhongzahong 318 bred by the Hemp Research Institute of the Chinese Academy of Agricultural Sciences, it was bred by the inventor through 6 generations of the system, ...

Embodiment 2

[0049] Embodiment 2: Breeding of restorer lines HR3 and HR4

[0050] On the basis of the materials used in Example 1, the added materials are:

[0051] 763A: Guikejianzidi [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, using UG93 as the cytoplasmic donor and Taihong 763 as the nuclear donor, bred by saturated backcrossing. The flowers are purple and the stems are light red.

[0052] 763B: Also known as Taihong 763, it is the reincarnated parent (nucleus donor parent) of 763A.

[0053] (1) In 2009, the cytoplasmic male sterile line 763A of kenaf was used as the female parent, and the cytoplasmic male sterile line 763A was propagated with 763B as the male parent; Kenaf hybrids 763A / Fuhong 992 and 763A / R1 were bred with 992 and R1 as male parents, respectively. Taking 763A as the female parent and 763A / Fuhong 992 and 763A / R1 as the male parent, two permanent segregation populations with a ratio of sterile to fertile plants o...

Embodiment 3

[0059] Embodiment 3: Breeding of restorer lines HR5 and HR6

[0060] On the basis of the materials used in Example 1, the added materials are:

[0061] 722A: Guikejianzidi [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, using UG93 as the cytoplasmic donor and 722 as the nuclear donor, bred by saturated backcrossing, completely Leaves, stems green.

[0062] 722B: also known as 722, a kenaf variety bred by the Cannabis Research Institute of the Chinese Academy of Agricultural Sciences, which is the reincarnation parent (nucleus donor parent) of 722A.

[0063] 917A: Guikejianzi [2004] No. 152, a cytoplasmic male sterile line selected by Mr. Zhou Ruiyang of Guangxi University, using UG93 as the cytoplasmic donor and 917 as the nuclear donor, bred by saturated backcross, split Leaves, stems light red.

[0064] 917B: also known as 917, a kenaf variety bred by the Hemp Research Institute of the Chinese Academy of Agricultural Sci...

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Abstract

The invention provides a method for breeding a restoring line or conventional variety by use of a cytoplasmic male sterile line of hibiscus cannabinus, which comprises the following steps: 1) performing outbreeding with corresponding male sterility maintainer line by taking the cytoplasmic male sterile line of hibiscus cannabinus as a female parent to obtain a cytoplasmic male sterile line; hybridizing with the restoring line by taking the cytoplasmic male sterile line as a female parent to obtain an F1-generation hybrid; and hybridizing by taking the F1-generation hybrid as a male parent and the cytoplasmic male sterile line of the female parent as a female parent to establish permanent segregation populations; 2) performing mixed planting of multiple permanent segregation populations, and classifying the sterile single plants with relatively consistent characters into one population to obtain multiple basic populations; 3) performing free pollination of each basic population; and after multiple generations, obtaining multiple strain populations from the strains without sterile plant separation and meeting the breeding target; and 4) identifying the strain population meeting the breeding target as the restoring line or conventional variety. The method provided by the invention performs free pollination by use of the cross-pollination characteristic of sterile line, and guarantees the universality of the hereditary basis of the restoring line or conventional variety.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for breeding restorer lines or conventional varieties by using kenaf cytoplasmic male sterile lines. Background technique [0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stems, the heterosis rate is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of domestic and foreign kenaf breeding. Utilization of valuable kenaf cytoplasmic male sterile lines for hybrid seed production. [0003] In September 2008, the "Breeding Method of Cytoplasmic Male Sterile Line of Kenaf Wild Abortive Type" invented by Zhou Ruiyang of Guangxi University won the national invention patent (ZL200510019454.4, publication number CN100415083C). According to this method, K03A (see: Guikejianzi No. [2004] No. 152), Fu 3A, P3A, 917A, 722A, 763A and L...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 周瑞阳陈鹏周琼赵艳红廖小芳周步进何冰
Owner GUANGXI UNIV
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