Method for Breeding Restorer Line or Conventional Variety Using Cytoplasmic Male Sterile Line of Kenaf

Abstract

The invention provides a method for breeding a restoring line or conventional variety by use of a cytoplasmic male sterile line of hibiscus cannabinus, which comprises the following steps: 1) performing outbreeding with corresponding male sterility maintainer line by taking the cytoplasmic male sterile line of hibiscus cannabinus as a female parent to obtain a cytoplasmic male sterile line; hybridizing with the restoring line by taking the cytoplasmic male sterile line as a female parent to obtain an F1-generation hybrid; and hybridizing by taking the F1-generation hybrid as a male parent and the cytoplasmic male sterile line of the female parent as a female parent to establish permanent segregation populations; 2) performing mixed planting of multiple permanent segregation populations, and classifying the sterile single plants with relatively consistent characters into one population to obtain multiple basic populations; 3) performing free pollination of each basic population; and after multiple generations, obtaining multiple strain populations from the strains without sterile plant separation and meeting the breeding target; and 4) identifying the strain population meeting the breeding target as the restoring line or conventional variety. The method provided by the invention performs free pollination by use of the cross-pollination characteristic of sterile line, and guarantees the universality of the hereditary basis of the restoring line or conventional variety.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for breeding restorer lines or conventional varieties by using kenaf cytoplasmic male sterile lines. Background technique [0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stems, the heterosis rate is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of domestic and foreign kenaf breeding. Utilization of valuable kenaf cytoplasmic male sterile lines for hybrid seed production. [0003] In September 2008, the "Breeding Method of Cytoplasmic Male Sterile Line of Kenaf Wild Abortive Type" invented by Zhou Ruiyang of Guangxi University won the national invention patent (ZL200510019454.4, publication number CN100415083C). According to this method, K03A (see: Guikejianzi No. [2004] No. 152), Fu 3A, P3A, 917A, 722A, 763A and L...

AI Technical Summary

Problems solved by technology

Because it requires artificial emasculation and hybridization, the workload is large; it also requires multiple generations of homozygous selfing, and the breeding cycle is long; and the number of parents is limited, resulting in a narrow genetic basis