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Turbot and platichthys stellatus crossbreeding method

A technology of Florida and Turbot, which is applied in the field of hybrid breeding of Turbot and Flounder, can solve problems such as unreported, and achieve the effects of low albino rate, fast growth and strong adaptability.

Inactive Publication Date: 2014-04-30
QINGDAO AGRI UNIV
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AI Technical Summary

Problems solved by technology

At present, there are many reports on the crossbreeding of flounder and flounder, but there is no report on the crossbreeding of turbot male × star spot river flounder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Determine the parents: the male parent is turbot, and the female parent is flounder.

[0022] 2) Breeding of male parents: 30 3-year-old turbot broodstock were selected for intensive breeding of broodstock, and the culture density was 1 fish / m 2 , The feeding water is formed by the deployment of sea water and well water, and the daily water exchange rate is 600%. Feed fresh miscellaneous fish, such as sterilized and chilled squid, scorpion fish, etc., twice a day (7:00, 16:00), according to the feeding situation, the daily feeding amount accounts for 1-3.5% of its body weight , light control light, light intensity 150-500lx, light time from 10L / 14D to 8L / 16D. The water temperature was gradually adjusted from 8°C to 12°C. 30 days before intensive cultivation, the gonads of one fish were taken every 10 days for visual inspection and tissue section analysis to determine the development of gonads, and compared with the development of gonads of the female parent. ℃, as ...

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Abstract

The invention discloses a turbot and platichthys stellatus crossbreeding method which mainly includes the steps of (1) a turbot milter is selected as a male parent, a platichthys stellatus female fish is selected as a female parent, and the male parent and the female parent mature at the same time by the adoption of different conditions; (2) artificial insemination is carried out, wherein the insemination rate reaches 70%; (3) fertilized eggs deciduate and incubate at incubation water temperature of 8+ / -0.5 DEG C in 80 hours, and the incubation rate is 82%; (4) larvae are cultured with intensified bait and then are cultured with compound feed gradually, and the survival rate of the larvae reaches 71%. According to the method, new varieties are provided for large-scale breeding of flatfish, and the method plays an important role in fish genetic breeding research.

Description

technical field [0001] The invention belongs to the technical field of seawater fish seed breeding, and in particular relates to a method for hybrid breeding of turbot and flounder, that is, an artificial crossbreeding method of male turbot x flounder with excellent quality. Breeding methods for new varieties of traits. Background technique [0002] turbot( Scophthamus maximus ) is a rare economic fish species in Europe. It adapts to low water temperature environment, grows fast, has strong stress resistance, tender meat, rich gelatin, and unique taste. It is deeply loved by farmers and consumers. Since it was introduced into my country in 1992, it has developed rapidly in the northern coast of my country and has become a pillar industry of mariculture in northern my country. Under artificial breeding conditions, domesticated turbot can reproduce throughout the year by controlling temperature and light. Star spotted flounder ( Platichthyus stellatus ) is a cold-temperat...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 方华华侯竹美
Owner QINGDAO AGRI UNIV
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