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Method for preparing metal-chelating peptide through dual-enzyme cooperative hydrolysis

A metal chelating peptide and enzymatic hydrolysis technology, applied in the biological field, can solve the problems of zinc absorption and metabolism limitation, zinc deficiency, etc.

Active Publication Date: 2014-05-14
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although zinc sources are very abundant in nature, the absorption and metabolism of zinc in the human body is restricted by various factors. Zinc deficiency has become a public health problem in my country and many developing countries.

Method used

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  • Method for preparing metal-chelating peptide through dual-enzyme cooperative hydrolysis
  • Method for preparing metal-chelating peptide through dual-enzyme cooperative hydrolysis
  • Method for preparing metal-chelating peptide through dual-enzyme cooperative hydrolysis

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Embodiment 1

[0017] The preparation method is as follows:

[0018] (1) Optimization of enzymatic hydrolysis conditions for marine source proteins

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Abstract

The invention provides a metal-chelating peptide, wherein the metal can be Ca, Fe and Zn, and the invention particularly relates to a method for preparing metal-chelating peptide through dual-enzyme cooperative hydrolysis. Ocean source fishskin and fish scale protein are used as the raw materials, compounding of compound protease and flavourzyme is used for carrying out enzymolysis on the raw materials, the metal-chelating peptide is obtained after separation, purification and freezing and drying, and the amino acid sequence of peptide is kngedg. Amino acid and small peptide have the effect of promoting zinc absorption, the biology utilization rate of metal compounds like Fe and Zn of amino acid or peptide is higher than that of inorganic salt, and the metal compounds have no toxic and side effects. Study and development of the biostate zinc-proteolysis chelating zinc have important significance.

Description

technical field [0001] The invention relates to a metal (Ca, Fe, Zn) chelating peptide, and more specifically relates to a method for preparing a metal chelating peptide by synergistic hydrolysis with two enzymes, which belongs to the field of biotechnology. Background technique [0002] Bioactive peptides derived from marine protein have extremely high nutritional value, food safety and physiological health functions, and play an important role in promoting the growth, development and disease prevention of the body, and can be used as functional food or food additives and even medicines applied to people's daily life. [0003] Calcium deficiency is a global nutritional problem. Because our people mainly eat plant-based diets, the phenomenon of calcium deficiency is even more serious. Therefore, calcium supplementation has become an important topic in the study of dietary nutrition in our country. Traditional inorganic calcium salts, such as calcium carbonate, calcium phosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12P21/06C07K1/20C07K1/18C07K1/16
CPCC07K14/461
Inventor 汪少芸唐梦茹何庆燕邵彪方卫东赵立娜江勇
Owner FUZHOU UNIV
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