Method for cultivating aseptic cordyceps militaris

A cultivation method and technology for Cordyceps militaris are applied in the cultivation of sterile Cordyceps militaris, fruiting bodies of Cordyceps militaris and the cultivation field thereof, which can solve the problems of unfavorable product development and utilization, large fluctuation of technical rigidity rate, and low average rigidity rate, etc. High effective ingredients, avoid pollution, resolve conflicts

Inactive Publication Date: 2014-05-21
山东省蚕业研究所
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the above-mentioned technologies all have large fluctuations in ossification rate and low average ossification rate. Silkworm Cordyceps contains a variety of other microorganisms, which is not conducive to the development and utilization of subsequent products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of cultivation method of aseptic silkworm caterpillar fungus, the steps are as follows:

[0041] (1) Wrap silkworm eggs that have been accelerated to the greening stage with gauze, soak in 75% (volume percentage) ethanol solution for 1 minute, transfer to 4% (volume percentage) formaldehyde solution for 8 minutes, and then transfer to Immerse in absolute ethanol for 1 minute, transfer to a sterile isolator to dry naturally, and continue to cyanogenize in the isolator until hatching;

[0042] The silkworm larvae were raised in a sterile isolator, and the sterile isolator used a YDG transparent plastic film isolator (100×60×60cm), which was composed of a plastic film chamber, a high-efficiency filter and an air supply system; Check whether there is any air leakage, clean it, spray it with 4% (volume percentage) formaldehyde solution, ventilate for 12 hours, and dry it until there is no formaldehyde smell before using it; the breeding temperature is 29°C for 1 to 3 ...

Embodiment 2

[0047] A kind of cultivation method of aseptic silkworm caterpillar fungus, the steps are as follows:

[0048] (1) Wrap silkworm eggs that have been accelerated to the greening stage with gauze, soak in 75% (volume percentage) ethanol solution for 2 minutes, transfer to 2% (volume percentage) formaldehyde solution for 10 minutes, and then transfer to Immerse in absolute ethanol for 2 minutes, transfer to a sterile isolator to dry naturally, and continue to cyanogenize in the isolator until hatching;

[0049] Bombyx mori larvae are raised in the 10,000-level purification standard silkworm room. The silkworm room construction adopts the 10,000-level purification standard of the barrier system and the full-composite sandwich color steel structure. It is also equipped with dressing room, shower room, air shower room, exit buffer room, auxiliary room, etc. All corners of the room are inlaid with aluminum alloy arc lines to reduce the retention of dust particles. The air purificati...

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Abstract

The invention relates to a method for cultivating aseptic cordyceps militaris. The method includes the following steps that firstly, silkworm eggs are sterilized and then incubated under the aseptic condition, and then aseptic silkworm bodies are acquired; secondly, cordyceps militaris fruiting bodies are cultivated in an aseptic environment, and then inoculant liquid is acquired; thirdly, the inoculant liquid is inoculated to caudal horns or prolegs or internodes of the aseptic silkworm bodies in an aseptic injection mode, the silkworm bodies are normally aseptically fed for two to three days, after litter cleaning is conducted on the rigescent silkworm bodies, the silkworm bodies are cultivated under the dark condition, then photoreception germination cultivation is conducted, and the aseptic cordyceps militaris is acquired after low-temperature drying. Cordyceps militaris is cultivated through aseptic silkworm larvae artificially fed in the aseptic environment, the influence of multiple microorganisms in the silkworm bodies on growth of the cordyceps militaris is avoided, compared with domestic silkworm larvae fed by common mulberry leaves, the aseptic silkworm larvae are higher in inoculation rigidity and germination rate, and the effective constituents of the cultivated cordyceps militaris are more.

Description

technical field [0001] The invention relates to a method for cultivating sterile Cordyceps militaris, in particular to a fruiting body of Cordyceps militaris using live sterile silkworm larvae as a host and a method for cultivating the fruiting body of Cordyceps militaris, which belongs to the technical field of edible fungus cultivation. Background technique [0002] Cordyceps militaris (La.: Fr), also known as Cordyceps militaris, belongs to the phylum Eumycota, the subphylum Aseomycotina, the order Hypocreales, the family Clavcipitaceae, and the genus Cordyceps ). It is another kind of caterpillar fungus different from Cordyceps sinensis that parasitizes on the pupae such as Noctuidae. [0003] The medicinal value of Cordyceps militaris: It is recorded in "Xinhua Compendium of Materia Medica" and "Chinese Medicine Sea". It has mild medicinal properties and has more medicinal and edible properties than other kinds of tonics. It is a high-grade tonic that can be used both...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor李化秀顾寅钰施新琴李峰于振诚郭光李智峰聂磊赵德安王玺兰
Owner山东省蚕业研究所