Tetracycline-containing medicine composition and application for same
A composition and technology of tetracycline, applied in the field of medicine, can solve the problems of missed treatment period, treatment cost, timely diagnosis and prevention of cancer, etc.
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Embodiment 1
[0051] Example 1: In vitro study evaluating the effect of the combination of tetracycline and bortezomib on the growth of lung cancer cells.
[0052] Cell Culture: The NSCLC cell line tested in the experiments was A549 cells obtained from ATCC. The cell culture medium used was RPMI medium 1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U / mL penicillin and 100 μg / mL streptomycin at 37 °C with a volume fraction of 5% CO 2 When cultured in an incubator, the cells grew adherently, and the cells in the logarithmic growth phase were used in the experiments.
[0053] Screening is done in two parts:
[0054] a. In the first set of experiments, the half inhibitory concentration (IC) of each drug was determined after being exposed to the A549 cell line for 72 hours 50 )value. Specific steps are as follows:
[0055] The logarithmic phase cells were collected, seeded in a 96-well plate, 100 μL was added to each well, and the concentration of the cell suspension was adj...
Embodiment 2
[0070] Example 2: In vitro study evaluating the effect of the combination of tetracycline and fludarabine on the growth of lung cancer cells.
[0071] Cells were cultured using the same method as in Example 1. Screening is done in two parts:
[0072] a. In the first group of experiments, using the same method as in Example 1, the half maximal inhibitory concentration (IC) of each drug was measured after being exposed to the A549 cell line for 72 hours. 50 )value. Draw a dose-response curve (Figure 1).
[0073] Table 4 provides the IC for each single agent in the combination of tetracycline and fludarabine 50 value.
[0074] Table 4
[0075] drug name
IC 50 (μM)
Tetracycline
42.8539
Fludarabine
0.25002
[0076] b. In the second set of experiments, using the same method as in Example 1, the inhibitory effect of tetracycline combined with fludarabine on the A549 cell line was determined ( FIG. 2 ).
[0077] Table 5 provides the C...
Embodiment 3
[0085] Example 3: In vitro study evaluating the effect of the combination of tetracycline and imatinib on the growth of lung cancer cells.
[0086] Cells were cultured using the same method as in Example 1. Screening is done in two parts:
[0087] b. In the first set of experiments, use the same method as in Example 1 to measure the half inhibitory concentration (IC) of each drug after being exposed to the A549 cell line for 72 hours. 50 )value. Draw a dose-response curve (Figure 1).
[0088] Table 7 provides the IC for each single agent in the combination of tetracycline and imatinib 50 value.
[0089] Table 7
[0090] drug name
IC 50 (μM)
Tetracycline
42.8539
Imatinib
50.1017
[0091] b. In the second set of experiments, using the same method as in Example 1, the inhibitory effect of tetracycline combined with imatinib on the A549 cell line was determined ( FIG. 2 ).
[0092] Table 8 provides the Combination Index (CI) obta...
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