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Method for purifying tanshinone IIA in salvia miltiorrhiza crude extract through simulated moving bed chromatography

A simulated moving bed and chromatographic purification technology, which is applied in the field of three-band simulated moving bed chromatography to purify tanshinone IIA, can solve the problems of large consumption of eluent, incapable of continuous automatic production, undiscovered problems, etc., and achieve the effect of reducing the cost of purification

Active Publication Date: 2014-07-23
UNIV OF SCI & TECH LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Chinese Patent Publication (Announcement) No.: CN1369485, which provides "a method for extracting and refining Tanshinone IIA with supercritical carbon dioxide", using supercritical CO 2 Extract tanshinone IIA from Salvia miltiorrhiza, extract and separate and refine at one time. It has the advantages of non-toxic, fast, low-cost, low-temperature operation, and environmental protection requirements. However, when extracting high-purity (higher than 90%) tanshinone IIA monomer, the harvest low rate
These column chromatography are all batch processes, which consume a lot of eluents and cannot be continuously and automatically produced
There is no report on the separation and purification of Tanshinone IIA by simulated moving bed chromatography

Method used

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  • Method for purifying tanshinone IIA in salvia miltiorrhiza crude extract through simulated moving bed chromatography
  • Method for purifying tanshinone IIA in salvia miltiorrhiza crude extract through simulated moving bed chromatography
  • Method for purifying tanshinone IIA in salvia miltiorrhiza crude extract through simulated moving bed chromatography

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Experimental program
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Effect test

Embodiment 1

[0043] A method for purifying tanshinone IIA by three-band simulated moving bed chromatography, using SMBC to purify tanshinone IIA from the ethanol extract of Salvia miltiorrhiza, the content of the method is as follows:

[0044] (1) SMBC equipment

[0045] SMBC equipment consists of 6 chromatographic columns, mobile phase D delivery pump, raw material liquid F pump, mobile phase P delivery pump, 36 automatic control valves, a set of single-chip automatic control system, 5 liquid storage tanks, multi-pass and connecting pipelines, Among them, the pump flow rate of the raw material F and the flow rate of the mobile phase P delivery pump are both 0-10mL / min, and the pressure is 0-30Mpa. The mobile phase D, raw material F, mobile phase P, extract E and raffinate R are respectively use a reservoir;

[0046] Chromatographic column specifications: diameter 1cm, length 10cm;

[0047] Octadecylsilane-bonded silica gel ODS, 20-30 μm was used as the stationary phase.

[0048] (2) SM...

Embodiment 2

[0062] Except following conditions, other conditions are all with embodiment 1;

[0063] Operating mode: 1-3-2;

[0064] Switching time Ts: 11min;

[0065] SMBC purification results: the chromatographic purity of the extract Tanshinone IIA was 93%, and the yield was 92%.

Embodiment 3

[0067] Except following conditions, other conditions are all with embodiment 1;

[0068] 5 chromatographic columns, 30 automatic control valves;

[0069] Chromatographic column specifications: diameter 2cm, length 20cm;

[0070] Operating mode: 1-2-2;

[0071] Stationary phase: octadecylsilane bonded silica gel ODS, 30-40 μm;

[0072] Mobile phase flow rate: eluent D flow rate Q D 6mL / min, flow rate Q of sample solution F F 0.8mL / min, eluent P flow rate Q p 6mL / min;

[0073] Switching time Ts: 19min;

[0074] SMBC purification results: the chromatographic purity of the extract Tanshinone IIA was 96%, and the yield was 92.7%.

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Abstract

The invention discloses a method for purifying tanshinone IIA in a salvia miltiorrhiza crude extract through simulated moving bed chromatography (SMBC). The method is characterized in that basic zones of the SMBC are an elution zone, a refinement zone and an adsorption zone, the elution zone is independent, octadecylsilane (ODS) bonded silica gel is used as a stationary phase, a mixed solution of alcohol and water is used as a mobile phase, the SMBC separation is carried out, and previous impurities are removed at a raffinate outlet; tanshinone IIA extract liquid is obtained at an extract liquid outlet, and the tanshinone IIA product with the purity of 98.5% is obtained after the tanshinone IIA extract liquid is concentrated and subjected to water sedimentation and alcohol dissolving treatment. The method can be used for stably, continuously and efficiently purifying the tanshinone IIA in salvia miltiorrhiza alcohol extract liquid on a large scale through SMBC; the highest recovery rate of the salvia miltiorrhiza IIV in the extract liquid reaches 99.2%; the HPLC purity reaches 95%; the stationary phase and the mobile phase can be repeatedly used; the cost is reduced; the method belongs to the environment-friendly separation process.

Description

technical field [0001] The invention relates to a method for purifying effective components of natural plants, in particular to a method for purifying tanshinone IIA by three-band simulated moving bed chromatography. Background technique [0002] Tanshinone IIA is one of the active ingredients of Salvia miltiorrhiza, and has very broad clinical application prospects. In addition to the traditional effects of promoting blood circulation, regulating menstruation, removing blood stasis, relieving pain, and nourishing the heart and calming the nerves, pharmacological studies in recent years have shown that tanshinone IIA also has anti-inflammatory, anti-oxidation, and anti-inflammatory properties. Tumor, antibacterial, liver protection and reduce blood viscosity, inhibit coagulation, promote fibrinolysis, inhibit platelet aggregation, prolong thrombus formation and promote thrombolysis, regulate immune function, improve anti-diabetic microvascular complications and other pharmaco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00
Inventor 王绍艳魏伯峰李守江张园齐婷洪鹤员
Owner UNIV OF SCI & TECH LIAONING
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