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Nattokinase composition capable of improving stability and oral curative effect and used for preventing thrombogenesis and thrombolysis

A technology of nattokinase and composition, which is applied in the field of nattokinase products, can solve problems such as destruction, loss of biological activity, and increase of antithrombotic effect of nattokinase, so as to improve oral drug efficacy, oral absorption and stability Effect

Active Publication Date: 2014-07-30
DELI WEI BEIJING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the present invention aims to solve the ubiquitous problems of NK from different sources (different microorganisms, recombinant proteins), that is, oral absorption, low stability of heat / pH acid / enzyme
It solves the technical problem that nattokinase is easily destroyed by gastric acid / gastrointestinal protease and loses its biological activity due to oral administration, thereby increasing the antithrombotic effect of nattokinase

Method used

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  • Nattokinase composition capable of improving stability and oral curative effect and used for preventing thrombogenesis and thrombolysis
  • Nattokinase composition capable of improving stability and oral curative effect and used for preventing thrombogenesis and thrombolysis
  • Nattokinase composition capable of improving stability and oral curative effect and used for preventing thrombogenesis and thrombolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The comparison of embodiment 1NK and DOPS / DOPC composition

[0075] According to the ratio (NK-PS; NK-PC ratio 100:10, w / w), the NK aqueous solution was added to the PS or PC tert-butanol water dispersion system, stirred and mixed, and freeze-dried. The obtained freeze-dried product was filled into rat-specific enteric-coated small capsules to obtain the test preparation.

[0076] 30 wistar rats were randomly divided into 5 groups, 6 in each group, and each group was as follows:

[0077] 1. Blank group (Control), fed with the same number of empty capsules as the administration group.

[0078] 2. NK-PS capsule low-dose group (NK-PS-L), the dosage is 10kIU·kg -1 .

[0079] 3. NK-PS capsule high-dose group (NK-PS-H), the dosage is 20kIU·kg -1 .

[0080] 4. NK-PC capsule low-dose group (NK-PC-L), the dosage is 10kIU·kg -1 .

[0081] 5. NK-PC high-dose group (NK-PC-H), the dosage is 20kIU·kg -1 .

[0082] 6. For the PS group alone, the dose is equivalent to the PS a...

Embodiment 2

[0094] The comparison of the different purity of embodiment 2 commercially available PS

[0095] Due to the high price of DOPS (the selling price of 10mg is 52 US dollars), it seriously hinders the marketization of the product, so the effect of increasing NK absorption with 20%, 40%, and 60% content of commercially available PS was compared. According to the ratio (the ratio of NK-PS or NK-PC is 100:10, W / w), the NK aqueous solution is added to the PS or PC tert-butanol aqueous dispersion system, stirred and mixed, and freeze-dried. The obtained freeze-dried product was filled into rat-specific enteric-coated small capsules to obtain the test preparation.

[0096] 30 wistar rats were randomly divided into 5 groups, 6 in each group, and each group was as follows:

[0097] 1. Blank group (Control), fed with the same number of empty capsules as the administration group.

[0098] 2. NK-PC capsule group (NK-PC), the dosage is 10kIU·kg -1 .

[0099] 3. NK-PS (20%) capsule group ...

Embodiment 3

[0110] Embodiment 3 Different ratio ranges

[0111] Use commercially available 60% PS, investigate different ratios, add NK aqueous solution to PS or PC tert-butanol water dispersion system, stirred and mixed, freeze-dried. The obtained freeze-dried product was filled into rat-specific enteric-coated small capsules to obtain the test preparation.

[0112] Sixty wistar rats were randomly divided into 12 groups, 5 rats in each group.

[0113] Administer once a day for 7 consecutive days. On the 8th day after the administration, the rats were intraperitoneally anesthetized with 3.5% chloral hydrate (1 ml / 100 g), and the bilateral common carotid arteries were surgically isolated. Place a 1.2cm×3.0cm plastic sheet under the bilateral common carotid arteries, and cover the bilateral common carotid arteries with a filter paper strip (1.0cm×1.5cm) impregnated with 50 μl FeCl3 solution (10%, w / w) . After 20 min, remove the filter paper strip, cut off the discolored area and wash i...

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Abstract

The invention provides a nattokinase composition capable of improving stability and oral curative effect and used for preventing thrombogenesis and thrombolysis. The nattokinase composition is characterized by comprising nattokinase and phosphatidylserine, wherein the weight ratio of the nattokinase to the phosphatidylserine is (100:1) to (100:1,000), preferably (100:1) to (100:100), and further preferably (100:3) to (100:30).

Description

[0001] Technical field: the present invention relates to a highly absorbed nattokinase product, in particular to a nattokinase composition for preventing thrombosis and thrombolysis and a manufacturing method thereof. Background technique: [0002] Nattokinase is a Bacillus subtilis protein kinase, which is a serine protease produced by Bacillus subtilis natto (Bacil lus subtilisl natto) during the fermentation process of natto. It has the functions of dissolving thrombus, reducing blood viscosity, improving blood circulation, softening and Increase blood vessel elasticity and other effects. [0003] In the 1980s, Hiroyuki Sumi (必见洋行), who was engaged in thrombus research in the United States, was looking for new antithrombotic substances in various natural products and found that the extract of natto viscous substance has a strong ability to dissolve artificial thrombus, and from it This kind of material is isolated as serine protease, i.e. Nattokinase (Nattokinase, NK) (Sumi...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61K47/24A23L1/305A23L2/52A61P7/02
Inventor 邓吉林吴莹吴宝珍邓红玲
Owner DELI WEI BEIJING BIOLOGICAL TECH
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