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A kind of w18o49 nanosphere peroxidase and its application

A technology of W18O49 and peroxidase, applied in the field of nanotechnology and enzyme catalysis, can solve the problems of poor chemical stability of the simulated enzyme, low catalytic activity of the simulated enzyme, and high catalytic activity of the simulated enzyme

Inactive Publication Date: 2016-06-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these nano-mimetic enzymes still have some shortcomings, such as: the catalytic activity of the mimetic enzymes based on carbon materials is very low; the mimetic enzymes of noble metals have high catalytic activity, but are expensive; the mimetic enzymes of the sulfide series have poor chemical stability and are easily oxidized Wait

Method used

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  • A kind of w18o49 nanosphere peroxidase and its application
  • A kind of w18o49 nanosphere peroxidase and its application
  • A kind of w18o49 nanosphere peroxidase and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0029] spherical W 18 o 49 The nanoparticle aqueous solution was ultrasonically treated for 0.5h, then dropped on a copper grid, and then scanned by a transmission electron microscope (TEM) after drying. Such as figure 1 Shown, W 18 o 49 Nanoparticles are self-assembled from many rods to form spherical particles with a uniform size of about 100nm.

Embodiment 2

[0031] At a temperature of 35°C, 50 μg / ml spherical W 18 o 49 Nanoparticles were added to 2.275ml of PBS buffer solution containing 0.6mM TMB, pH = 4.0, and 50mM H 2 o 2 solution, reacted for 30min, and measured the absorbance at 652nm on a UV spectrophotometer.

[0032] from figure 2 It can be observed that the experimental group has a very obvious absorption at 652nm, indicating that the spherical W18 o 49 Nanoparticles can catalyze H 2 o 2 , to oxidize TMB to form oxTMB.

Embodiment 3

[0034] At room temperature, 50 μg / ml spherical W 18 o 49 Nanoparticles and 0.6mM MTB solution were added to 2.275ml of PBS buffer solution with different pH values ​​(respectively pH = 3.0, 4.0, 5.0, 6.0, 7.0), and finally 50mM H 2 o 2 , react at room temperature for 30min, measure the absorbance at 652nm on a UV spectrophotometer, as image 3 shown.

[0035] image 3 The results show that the absorption at 652nm is the highest at pH=4.0, indicating that the optimal pH of the reaction is 4.0, that is, the slightly acidic environment is favorable for W 18 o 49 Nanoparticles catalyze the color reaction of TMB.

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Abstract

The invention provides a W18O49 nanosphere peroxidase analogue and application thereof. The fact that W18O49 nanoparticles have the functions of an peroxidase analogue is reported for the first time; the W18O49 nanoparticles can be used for catalyzing an H2O2 oxidation substrate to perform color development reaction; the catalytic activity of the W18O49 nanoparticles is sourced from oxygen vacancies in the surfaces; the W18O49 nanoparticles belong to a novel peroxidase analogue. The nano-material has the advantages of being simple in preparation process, stable in chemical activity, high in special surface area and the like, so that the nano-material has the better catalytic effect and the higher applicability than those of natural peroxidase, can serve as a substitute of the natural peroxidase, and is applied to the degradation of organic pollutants in environment sewage.

Description

Technical field: [0001] The invention belongs to the field of nanotechnology and the field of enzyme catalysis, and relates to a W 18 o 49 Nanosphere peroxidase and application thereof, specifically related to W 18 o 49 Nanoparticles have peroxidase-like activity and catalytic applications. Background technique [0002] Biological enzymes widely exist in nature and play an important role in biology, medicine, chemical industry, environment and other fields. Biological enzymes have the advantages of high catalytic activity, specificity, and mild reaction conditions. However, since most of the natural biological enzymes are proteins, they are not resistant to high temperature and acid and alkali, and the synthesis process of natural biological enzymes is relatively complicated and the cost is relatively high. Therefore, it is of great significance to search for efficient artificial mimetic enzymes. [0003] Since Fe 3 o 4 It is the first report that nanomaterials have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J23/30C02F1/72C02F101/30
CPCY02W10/37
Inventor 朱春玲占红梅李子彬谢增鸿
Owner FUZHOU UNIV