Backbone plasmid vector and application thereof

Abstract

The invention discloses a backbone plasmid vector. In the backbone plasmid vector, a guide RNA (Ribonucleic Acid) expression cassette and a Cas9 nuclease expression cassette are located in the same binary vector; the guide RNA expression cassette is sequentially composed of a wheat TaU3p promoter, a spectinomycin resistance gene, an artificially-synthesized sgRNA framework sequence and a Poly-T terminator; the Cas9 nuclease expression cassette is sequentially composed of a ZmUBI promoter, a plant preferred codon modified Cas9 coding sequence and a 35s terminator. The invention also provides a target-sequence-contained recombinant vector constructed by using the backbone plasmid vector and used for crop gene targeting.

Description

technical field [0001] The invention belongs to the field of crop genetic engineering, and in particular relates to a backbone plasmid carrier for crop gene targeting and its application in genetic modification. Background technique [0002] my country is a large agricultural country, and the sustained and stable increase in the production of major agricultural products is of great strategic significance to ensure my country's food security. Seeds are the source of food production, and excellent varieties are an important basis for ensuring high, high-quality, and stable agricultural yields. With the continuous development of the national economy, the continuous increase of the population and the frequent occurrence of extreme weather, it is very important to accelerate the improvement of varieties to stabilize the output of agricultural products and meet the needs of modern agriculture in the development of the times. Since the middle of the last century, traditional breed...

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Problems solved by technology

However, in the research on rice, the current limited reports mainly focus on the mechanism research, and there is no rep

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