Analysis method for diversity of microbes and abundance of species in coal seam water

Abstract

The invention belongs to the field of research on microbes in stratum water and provides an analysis method for diversity of microbes and abundance of species in coal seam water to overcome the problems that research on diversity of microbes in an environment has great limitation, species with low abundance in a sample cannot be effectively detected, species with high similarity in the sample are difficult to distinguish, only a minority of microbe species in the sample can be revealed, etc. The method comprises the following steps: acquisition of a coal seam water sample; enrichment of microbial thalli in the water sample and extraction of total DNA of the microbial thalli; amplification of the hypervariable region of 16SrDNA with specific primers; sequencing analysis with an Illumina sequencing platform; and processing of original data of sequencing so as to obtain diversity and abundance of microbes in the water sample. The method can effectively detect species with low abundance in the sample, identifies more than 400 genuses of bacteria in coal seam water, has obvious superiority in research on species and abundance of microbes in coal seam water and is applicable to analysis of diversity of microbes and abundance of species in environments like coal seams and oil gas or shale gas strata.

Description

technical field [0001] The invention belongs to the research field of formation water microorganisms, in particular to a method for analyzing the diversity and species abundance of microorganisms in coal seam water. Background technique [0002] Coalbed gas, commonly known as gas, is a self-storage natural gas generated during coal formation and stored in coal seams and surrounding rocks in an adsorbed and free state, in which the volume fraction of CH4 is up to 95%. According to the 2006 national oil and gas resource evaluation, the coalbed methane resources with a buried depth of less than 2000 m in my country reached 36.81×1012 m 3 , which is basically equivalent to the prospective resources of natural gas in my country. With the rapid growth of the national economy, my country's demand for energy is increasing. As unconventional natural gas, coalbed methane is an important supplementary source of natural gas resources in my country. The development and utilization of ...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the limitations of existing research on microbial diversity in the environment, the present invention requires a large amount of samples, cumbersome operation, time-consuming and laborious, low sensitivity, and cannot effectively detect species with low abundance in samples. It is difficult to distinguish species with high similarity in the sample, and only a small part of the microbial species in the sample can be revealed, providing an analysis method for microbial diversity and species abundance in coal seam water

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