Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tetrodotoxin positive quality control sample as well as preparation method and application thereof

A technology of puffer toxin and positive quality control, which is applied in the field of food safety testing and achieves the effects of long storage time and simple preparation.

Inactive Publication Date: 2014-08-13
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical and chemical properties of this kind of insoluble in any kind of neutral organic solvent, as well as the complex matrix effect of puffer fish tissue make the quantitative determination of tetrodotoxin a challenge in the fields of food testing, ecology and medical research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tetrodotoxin positive quality control sample as well as preparation method and application thereof
  • Tetrodotoxin positive quality control sample as well as preparation method and application thereof
  • Tetrodotoxin positive quality control sample as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0035] The additive formula is C 18 Powder, additive formula is C 18 , The ratio of graphitized carbon and diatomaceous earth is 2:2;1, but not limited thereto.

Embodiment 1

[0037] Sample pretreatment

[0038] 1) Live pufferfish were killed by freezing, peeled off the skin, and the muscle part was taken to remove tendons and thoroughly pulverized by a homogenizer.

[0039] 2) Weigh (1.00±0.01) g of the sample into a 50 mL polypropylene centrifuge tube, accurately add 5 mL of 1% acetic acid methanol (1:99, v / v) solution, homogenize for 30 s, and ultrasonically extract at 50 ° C for 25 min.8000r Centrifuge at / min*5min, transfer the supernatant to another clean centrifuge tube; repeat the extraction of the residue with 4mL1% acetic acid methanol solution once, combine the supernatant and centrifuge at 12000r / min at 4°C for 5min, the obtained supernatant is to be purified;

[0040] 3) Take 1.0mL supernatant to a 2mL EP (Eppendorf) tube, add 100mg graphitized carbon black (GCB) and 100mg C 18 The powder was vigorously vortexed for 30s, centrifuged at 10000r / min for 5min, and the supernatant was filtered through a 0.22μm polytetrafluoroethylene-nylon ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a tetrodotoxin positive quality control sample. The method comprises the steps of selecting muscular tissues from fresh fugu rubripes, homogenizing, and measuring the content of tetrodotoxin in the muscular tissues to verify whether the muscular tissues contain the tetrodotoxin; if the muscular tissue is a positive sample, diluting the positive sample containing toxin with a negative sample and a dispersant, mixing uniformly, continuously measuring the content of the toxin in the sample, packaging the sample after the sample meets requirements, preserving at an appropriate temperature, and performing uniformity verification and stability analysis on the sample; if the muscular tissue is a negative sample, adding a tetrodotoxin standard solution into the negative samples, adding a dispersant, mixing uniformly, measuring the content of the tetrodotoxin in the sample, packaging the sample after the sample meets requirements, preserving at an appropriate temperature, and performing uniformity verification and stability analysis on the sample. The quality control sample is simple to operate, and has enough stability and uniformity and long preservation time, so that the quality control sample can be used for laboratory quality control.

Description

technical field [0001] The invention relates to a tetrodotoxin (TTX) positive quality control sample and a preparation method thereof, belonging to the technical field of food safety detection. Background technique [0002] Pufferfish (including Fugu rubripes and Fugu obscurus, etc.) is currently a large-scale artificially cultured puffer species. The fish meat is rich in protein and lipid, and after detoxification treatment it becomes Precious delicacies. In order to monitor puffer fish toxicity in different seasons, quickly and accurately determine the TTX content in puffer fish tissues, distinguish toxic puffer fish from non-toxic puffer fish, and understand the toxicity differences and changes of common puffer fish in real time, it is necessary to establish a A fast, convenient, high-sensitivity, and highly operable TTX detection method, and the preparation of a practical tetrodotoxin positive quality control sample can be detected simultaneously with the actual sample ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 曹文卿林黎明吴振兴杨桂朋
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com