Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving salt tolerance of codonopsis pilosula

A technology of salt tolerance and Codonopsis pilosula, which is applied in the field of planting Chinese herbal medicines, can solve the problems of difficult promotion and cultivation of Codonopsis pilosula and weak salt tolerance of Codonopsis pilosula, achieve non-pathogenicity and strong stress resistance, increase emergence rate, and simple method Effect

Inactive Publication Date: 2014-08-27
LANZHOU UNIVERSITY
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Codonopsis pilosula has weak salt tolerance and is sensitive to salinized soil. However, most of the cultivated land in my country is seriously salinized, which makes it difficult for Codonopsis pilosula to be widely cultivated.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving salt tolerance of codonopsis pilosula
  • Method for improving salt tolerance of codonopsis pilosula
  • Method for improving salt tolerance of codonopsis pilosula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds in 75% alcohol for 10 seconds, soak them in 5% sodium hypochlorite for 10 minutes, rinse them with sterilized distilled water for 7 times, and put them in a refrigerator at 2-4°C overnight;

[0022] Preparation of Bacillus subtilis GB03 bacterial liquid: first prepare the lysis broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, and the high temperature to 132 Sterilize at ℃, after the cooling temperature is 20℃, add 50μL of Bacillus subtilis GB03 bacterial solution per 100mL of LB liquid medium, and cultivate overnight on a constant temperature culture shaker at 25℃ at a rotation speed of 250r / min. Determination of bacterial concentration as OD 600 When it is 0.8-1.0, take it out for use;

[0023] Seed bacterial solution treatment: soak the sterilized seeds in the Bacillus s...

Embodiment 2

[0025] Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds in 75% alcohol for 8 seconds, soak them in 5% sodium hypochlorite for 12 minutes, rinse them with sterilized distilled water 8 times, and put them in a refrigerator at 2-4°C overnight;

[0026] Preparation of Bacillus subtilis GB03 bacterial liquid: first prepare the lysis broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, and the high temperature to 115 Sterilize at ℃, after the cooling temperature is 35℃, add 100μL of Bacillus subtilis GB03 bacterial solution per 100mL of LB liquid medium, and cultivate overnight on a constant temperature culture shaker at 30℃ at a rotation speed of 180r / min. Determination of bacterial concentration as OD 600 When it is 0.8-1.0, take it out for use;

[0027] Seed bacterial solution treatment: soak the sterilized seeds in the Bacillus subti...

Embodiment 3

[0029] Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds in 75% alcohol for 10 seconds, soak them in 5% sodium hypochlorite for 10 minutes, rinse them with sterilized distilled water for 7 times, and put them in a refrigerator at 2-4°C overnight;

[0030] Seed bacterial liquid treatment: Take an appropriate amount of farmland soil and mix evenly with the sterilized seeds to ensure that the soil particles are loose and ready for use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving the salt tolerance of codonopsis pilosula. The method comprises the steps of soaking codonopsis pilosula with bacillus subtilis GB03 solution beneficial to soil before sowing and sowing the codonopsis pilosula to separate the seeds, and sowing the seeds. By adopting the scheme, the codonopsis pilosula seeds are treated before being sowed, so that the salt tolerance of codonopsis pilosula can be effectively improved, the emergence rate and the growth vigor of the codonopsis pilosula seeds in salinization soil can be remarkably improved, the growth of the codonopsis pilosula root system is improved and the yield is increased; meanwhile, the bacillus subtilis has the characteristics of no poison and no harm to people and livestock, incapability of polluting the environment, no pathogenicity and strong stress resistance; the method for treating the codonopsis pilosula seeds is simple, can be widely applied to codonopsis pilosula cultivation in mild and medium salinization soil, and has important economic, social and ecological values.

Description

technical field [0001] The invention relates to the field of planting Chinese medicinal materials, in particular to a method for improving the salt tolerance of Codonopsis pilosula. Background technique [0002] Codonopsis pilosula, also known as yellow ginseng, is produced in Shanxi, Gansu, Shaanxi, Sichuan, Hubei and other places in my country. At present, Codonopsis pilosula is mainly cultivated artificially, and a small amount is wild. Among them, Gansu is the largest production area of ​​Codonopsis pilosula. In recent years, Codonopsis pilosula has made new progress in the treatment of coronary heart disease, hyperlipidemia, hypotension, etc., and its usage has increased rapidly. The production and export of Codonopsis pilosula are growing steadily, and the shortage of Codonopsis medicinal materials has become a major problem in the production of traditional Chinese medicine. Codonopsis pilosula has weak salt tolerance and is sensitive to salinized soil. However, most ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 张金林吴永娜许瑞韩庆庆吕昕培王引权王锁民
Owner LANZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com