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Method for screening active components from traditional Chinese medicine or natural medicine complex system and purposes thereof

A technology of natural medicines and active ingredients, applied in pharmaceutical formulations, preparations for in vivo tests, etc., can solve the problem of inability to provide information on the pharmacodynamics of traditional Chinese medicine or natural medicines, and the inability to combine chemical composition research and pharmacodynamics research. And other issues

Active Publication Date: 2014-09-24
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Qualitative and quantitative analysis methods of chemical components of traditional Chinese medicine or natural medicine compound based on liquid chromatography-mass spectrometry are widely used in basic material research and as an effective tool for evaluating the quality standards of traditional Chinese medicine, but they cannot provide pharmacodynamic information of traditional Chinese medicine or natural medicine compound , it is impossible to organically combine the research on the chemical composition of the compound formula with the research on pharmacodynamics

Method used

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  • Method for screening active components from traditional Chinese medicine or natural medicine complex system and purposes thereof
  • Method for screening active components from traditional Chinese medicine or natural medicine complex system and purposes thereof
  • Method for screening active components from traditional Chinese medicine or natural medicine complex system and purposes thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Screening active ingredients from Chinese medicine compound

[0030] Extraction Example

[0031] Extraction Example 1. Sample Preparation of Chinese Medicine Compound (Bupleurum Shugan Powder)

[0032] 1. The medicinal materials and doses of the whole prescription of Chaihu Shugan San: 6 grams of Bupleurum, 6 grams of tangerine peel (stir-fried with vinegar), 4.5 grams of Chuanxiong, 4.5 grams of Citrus aurantium (stir-fried with bran), 4.5 grams of peony, 4.5 grams of Cyperus, Licorice (burnt) 1.5g

[0033] 2. Preparation method: Mix the above medicinal materials, crush them into coarse powder, add water 1:10, soak for 30 minutes, decoct for 1 hour, filter while hot, and decoct the residue for 1 hour again, combine the filtrates, concentrate under reduced pressure at 60°C, and freeze Dry to obtain Bupleurum Shugan San extract (CSGS).

Embodiment 2

[0034] Extraction Example 2. Sample Preparation of Compound Reduced Flavor Recipe (Fructus Aurantii Fructus Recipe)

[0035] 1. Composition and doses of Aurantium Fructus Recipe: 6 grams of Bupleurum, 6 grams of Chenpi (stir-fried with vinegar), 4.5 grams of Chuanxiong, 4.5 grams of Peony, 4.5 grams of Rhizoma Cyperus, 1.5 grams of Licorice (burned)

[0036] 2. Preparation method: The above-mentioned medicinal materials in the total amount ratio were prepared according to the method of Extraction Example 1 to obtain the extract of Bupleurum Shugan Sanjian Fructus Fructus Fructus Fructus (QZ).

experiment example

[0038] 1. Rat chronic unpredictable stress model replication and drug treatment

[0039] (1) Experimental animals and groups

[0040] Wistar rats, male, weighing 180-220 g, were purchased from Weitong Lihua Experimental Animal Co., Ltd., license: SCXK (Beijing) 2002-2003.

[0041] Animals were divided into 4 groups, 8 animals in each group: blank control group (Control group): no stimulation was given, and normal saline was given; pathological model group (CUMS group): stimulation was given while normal saline was given; Chaihu Shugan powder was administered Group (CSGS group): Give stimulation and give simultaneously the medicine liquid (administration dose 7.2g crude drug amount / Kg) that prepares medicine powder according to extracting the formula ratio of Example 1 and prepares as administration sample; Reduced taste prescription administration group (QZ group) : Give stimulus and give simultaneously the medicinal liquid (administration dose 6.7g crude drug amount / Kg) that...

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Abstract

The invention relates to a method for screening active components from a traditional Chinese medicine or natural medicine complex system, and provides the method for screening the active components from the traditional Chinese medicine or natural medicine complex system on the basis of metabonomics and traditional Chinese medicine chemical composition analysis technology. The method is characterized in that: establishing a related disease animal model, performing intervention therapy on effective parts by use of a traditional Chinese medicine or a natural medicine compound prescription or an element-plus / minus prescription of the traditional Chinese medicine or the natural medicine compound prescription; aiming at drug intervention-treated animal body fluids or tissues for metabonomics study to obtain curative effect differences information of medicine treatment groups; using a multivariate statistical analysis method to for search and identification of chemical constituents of differences so as to lead compounds or compound groups with active effects in the traditional Chinese medicine or natural medicine compound prescription. The method for screening the active components is reasonable and feasible. The method for screening the active components can be used as a method to develop new drugs from the traditional Chinese medicine or natural medicine complex system, also can be used as supplementary means for quality control of traditional Chinese medicines or natural medicine compound medicines.

Description

technical field [0001] The invention relates to a method for screening active ingredients in a complex system of traditional Chinese medicines and natural medicines and its application, in particular to the field of screening of active ingredients of Chinese medicines and the research field of mechanism of action, and in particular to a screening method and application of active ingredients of compound Chinese medicines or natural medicines. Background technique [0002] Traditional Chinese medicine or natural medicine compound is the main form of clinical medicine of traditional Chinese medicine, has a long-term folk application basis, and is the main source of new drug research and development. Modern medical research has shown that due to the complex and diverse components of traditional Chinese medicine or natural medicine compound, they exert therapeutic effects in the form of multiple channels, multiple targets, and multiple receptors. At present, the method of screeni...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 邹忠梅贾红梅苏志恒刘月涛丁刚张宏武常星
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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