Method for culturing bletilla striata protocorm by means of bletilla striata seeds according to fluid suspension culture method
A technology of suspension culture and protocorms, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as difficulty in securing seeds, differences in growth conditions, cumbersome transfer operations, etc., and achieve neat emergence, low cost, and easy operation simple effect
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[0025] The present invention will be described in detail below in conjunction with specific embodiments.
[0026] 1 Materials and methods
[0027] 1.1 Materials
[0028] 1.1.1 The experimental materials are white and ( Bletilla striata (Thunb.) Rchb. f.) mature capsule of the year. (Provided by Li Linhua, teacher of Anhui Traditional Chinese Medicine College, Wuhu City, Anhui Province, the fruit was collected in Yashan, Nanling County, Wuhu City, Anhui Province)
[0029] 1.1.2 Reagents, instruments and equipment 6-Benzylaminopurine (6-BA) was purchased from Shanghai Wanjiang Biotechnology Co., Ltd., 1-Naphthaleneacetic acid (NAA) was purchased from Jiangsu Qiangsheng Functional Chemicals Co., Ltd.; ESCO Company OptiMair ultra-clean workbench; Shanghai Shen'an Medical Instrument Factory autoclave; electronic balance provided by Shanghai Haikang Electronic Instrument Factory; dissecting mirror is Olympus CX41-12C02 binocular microscope CX41 Shanghai Zeshi Photoelectric T...
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