A kind of method for cultivating protocorms by suspension culture of bletilla seed liquid
A suspension culture and protocorm technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as difficulty in securing seeds, cumbersome transfer operations, and differences in growth conditions, and achieve neat emergence, low cost, and easy operation simple effect
Inactive Publication Date: 2016-05-04
中国科学院南京分院东台滩涂研究院 +2
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[0004] The existing dialogue and tissue culture all use solid medium. When inoculating, the seeds of the white and the seeds are gently shaken off the surface of the medium. However, because the seeds of the white and the seeds are powdery and very small, it is difficult to ensure that each seed can be fully absorbed during operation. Contact with the culture medium or the base of the embryo is in contact with the culture medium, resulting in differences in growth conditions, resulting in irregular seedling emergence, and the transfer operation is cumbersome and the workload is huge
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[0025] The present invention will be described in detail below in conjunction with specific embodiments.
[0026] 1 Materials and methods
[0027] 1.1 Materials
[0028] 1.1.1 The material used in the experiment is the mature capsule of Bletilla striata (Thunb.) Rchb.f.). (Provided by Li Linhua, teacher of Anhui Traditional Chinese Medicine College, Wuhu City, Anhui Province, the fruit was collected in Yashan, Nanling County, Wuhu City, Anhui Province)
[0029] 1.1.2 Reagents, instruments and equipment 6-Benzylaminopurine (6-BA) was purchased from Shanghai Wanjiang Biotechnology Co., Ltd., 1-Naphthaleneacetic acid (NAA) was purchased from Jiangsu Qiangsheng Functional Chemical Co., Ltd.; ESCO Company OptiMair ultra-clean workbench; Shanghai Shen'an Medical Instrument Factory autoclave; electronic balance provided by Shanghai Haikang Electronic Instrument Factory; dissecting mirror is Olympus CX41-12C02 binocular microscope CX41 Shanghai Zeshi Photoelectric Technology Co., L...
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The invention discloses a method for culturing bletilla striata protocorm by means of bletilla striata seeds according to the fluid suspension culture method. The method for culturing the bletilla striata protocorm by means of the bletilla striata seeds according to the fluid suspension culture method comprises the following steps that (1) the bletilla striata seeds are sterilized; (2) suspension germination cultivation is conducted, wherein under the sterile condition, a rhizoma bletillae capsule shell is cut open through scissors, and the seeds fall into a liquid germination culture medium through shaking and are cultured for thirty days in a shaking mode; (3) suspension culture of strong seedlings is conducted, wherein the bletilla striata protocorm obtained by conducting suspension germination cultivation in the step (2) is transferred into a liquid strong seedling culture medium and is cultured for 30-45 days in a shaking mode. According to the method for culturing the bletilla striata protocorm by means of the bletilla striata seeds according to the fluid suspension culture method, the fluid suspension culture method is adopted, the bletilla striata protocorm can be obtained effectively, operation is easy, cost is low, and the culture cycle can be effectively shortened.
Description
technical field [0001] The invention relates to a technique for raising seedlings of bletilla and in particular to a method for cultivating bletilla and protocorms by adopting a liquid suspension culture method. Background technique [0002] White and (Bletillastriata (Thunb.) Rchb.f.) is a perennial herbaceous plant belonging to Orchidaceae, with thick fleshy pseudobulbs for medicinal purposes; racemes with several flowers, purple or light red flowers, large flowers , about 5 cm in diameter, beautiful in shape, with high medicinal value and ornamental value. [0003] Baiji mainly reproduces by ramets in the natural state, and the reproduction coefficient is low; although it can produce a large number of seeds, the seeds are not easy to germinate. Due to the low reproduction rate of white and white, it cannot adapt to the needs of large-scale planting. At present, the researches on the reproduction of Bletillae mainly focus on aseptic assisted seedling cultivation and tiss...
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IPC IPC(8): A01H4/00
Inventor周义峰汤兴利刘彦红吴亚萍王玫
Owner中国科学院南京分院东台滩涂研究院