Constant-pressure transmembrane clamp for hybridization of Southern imprinting

A technology for transferring membrane and imprinting, which is applied in the field of constant pressure membrane transfer clips for Southern imprinting hybridization, can solve the problems such as the inability to quickly replace the absorbent paper, the inability to achieve the transfer membrane effect, and the inability to digitally and accurately adjust the pressure. Stable pressure and easy operation

CN104152342AActive Publication Date: 2014-11-19INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-11-19

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Abstract

The invention relates to a constant-pressure transmembrane clamp for hybridization of Southern imprinting. The constant-pressure transmembrane clamp comprises a variable-pressure solution storage tank 1, wherein a pressure gauge 2 is fixedly arranged at the bottom of the variable-pressure solution storage tank 1, an objective table 3 is fixedly arranged on the surface of the pressure gauge 2, a clamping plate 4 is arranged above the objective table 3 and can move up and down, holes are formed in two ends of the clamping plate 4, the clamping plate 4 with the holes and the variable-pressure solution storage tank 1 are in sliding connection with each other through lengthened screw rods 6 with detachable rotating nuts 5, the lengthened screw rods 6 with the nuts are fixedly arranged at two sides of the solution storage tank 1, the middle position of the lower part of the clamping plate 4 with the holes is fixedly connected with a hydraulic telescopic rod 8 with a bolt 7, the lower end of the hydraulic telescopic rod 8 is fixedly connected with a pressure plate 9, and a graduated scale 10 is parallel to the screw rod 6 and is fixedly connected with the rear side of the solution storage tank 1. The digitally accurately determined pressure acting on gel can be realized through the pressure gauge arranged at the bottom of the solution storage tank, so as to keep the pressure in the transmembrane process stable. In addition, the fast and convenient replacement of water-absorbing paper under constant-pressure conditions can also be realized, and the optimal transmembrane state can be achieved.
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Description

technical field

[0001] The invention relates to the technical field of transfer membrane devices, and more specifically, the invention relates to a constant pressure transfer membrane clip for Southern blot hybridization. Background technique

[0002] Southern blot hybridization (Southern blot) was created by the British southerner in 1975. It is a basic technique for studying DNA maps and is of great value in the diagnosis of genetic diseases, DNA map analysis and PCR product analysis. Southern blot hybridization is a classic experimental method in molecular biology. Its basic principle is to immobilize the DNA sample to be detected on a solid-phase carrier, hybridize with a labeled nucleic acid probe, and perform a hybridization on the solid-phase DNA with a homologous sequence to the probe. The hybridization signal is displayed at the position. Through Southern hybridization, it can be judged whether there is a fragment homologous to the probe and the length of the fragme...

Examples

Embodiment Construction

[0016] Such as figure 1 As shown, the constant pressure transfer clamp for Southern blot hybridization of the present invention includes a variable pressure liquid storage tank 1 with a pressure gauge 2 fixedly arranged at the bottom, a stage 3 is fixedly arranged on the surface of the pressure gauge 2, and a stage 3 is arranged above the stage 3 A splint 4 with holes at both ends that can move up and down. The splint 4 with holes is connected to the variable pressure liquid storage tank 1 through an extended screw 6 with a detachable rotating nut 5. The nut 5 is used to fix the splint 4 The lengthened screw rod 6 with a nut is fixedly arranged on both sides of the liquid storage tank 1, and the middle position of the lower part of the splint with a hole 4 is fixedly connected with a hydraulic telescopic rod 8 with a bolt 7, and the bolt 7 is used to adjust the position of the hydraulic telescopic rod 8. The telescopic length, the lower end of the hydraulic telescopic rod 8 is...