Tissue Culture and Rapid Propagation Method of Lagerstroemia yunnanensis
A technology of tissue culture and rapid propagation, Lagerstroemia indica, applied in the field of plant tissue culture, to achieve the effect of convenient transportation, efficient tissue culture and rapid propagation method, and easy acquisition
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Embodiment 1
[0031] The tissue culture rapid propagation method of embodiment 1 Lagerstroemia yunnanensis
[0032] 1. From November to December, when the seeds of Lagerstroemia yunnanensis are ripe, the fruits that have been lignified but not cracked are harvested ( figure 1 ), brought back to the laboratory, and stored in a refrigerator at 4°C for 2 weeks. Soak in 100 times diluted detergent spirit for 30 minutes, use a cleaning ball to grind off the peel, then rinse under running water for more than 2 hours, sterilize with 75% alcohol in an ultra-clean workbench for 1 minute, and sterilize with 4% sodium hypochlorite solution for 30 minutes. Rinse with water 5-6 times.
[0033] (2) Cut open the sterilized fruit, take out the seeds, and cut off the seed wings with a sterile scalpel to expose the seeds. Inoculate into 1 / 2MS basic medium containing 0.5g / L activated carbon, cultivate in dark for about 1-2 weeks, the seeds begin to germinate seedlings, and then transfer to culture under lig...
Embodiment 2
[0040] The tissue culture rapid propagation method of embodiment 2 Lagerstroemia yunnanensis
[0041] (1) From November to December, when the seeds of Lagerstroemia yunnanensis are ripe, harvest the lignified but uncracked fruits, bring them back to the laboratory, and store them in a refrigerator at 4°C for 2 weeks. Soak in 100 times diluted detergent spirit for 30 minutes, use a cleaning ball to grind off the peel, then rinse under running water for more than 2 hours, sterilize with 75% alcohol in an ultra-clean workbench for 1 minute, and sterilize with 4% sodium hypochlorite solution for 30 minutes. Rinse with water 5-6 times.
[0042] (2) Cut open the sterilized fruit, take out the seeds, and cut off the seed wings with a sterile scalpel to expose the seeds. Inoculate into 1 / 2MS basic medium containing 0.5g / L activated carbon, culture in dark for about 1-2 weeks, the seeds begin to germinate seedlings, and then transfer to light culture.
[0043] The basic medium is: 1 / ...
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