Tissue Culture and Rapid Propagation Method of Lagerstroemia yunnanensis
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A technology of tissue culture and rapid propagation, Lagerstroemia indica, applied in the field of plant tissue culture, to achieve the effect of convenient transportation, efficient tissue culture and rapid propagation method, and easy acquisition
Active Publication Date: 2016-04-27
BEIJING FORESTRY UNIVERSITY
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[0005] Since Lagerstroemia yunnanensis is a unique tree species in my country, it is only distributed in Simao, Lancang and other places in Yunnan, so the research on Lagerstroemia in Yunnan is still blank
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Embodiment 1
[0031] The tissue culture rapid propagation method of embodiment 1 Lagerstroemia yunnanensis
[0032] 1. From November to December, when the seeds of Lagerstroemia yunnanensis are ripe, the fruits that have been lignified but not cracked are harvested ( figure 1 ), brought back to the laboratory, and stored in a refrigerator at 4°C for 2 weeks. Soak in 100 times diluted detergent spirit for 30 minutes, use a cleaning ball to grind off the peel, then rinse under running water for more than 2 hours, sterilize with 75% alcohol in an ultra-clean workbench for 1 minute, and sterilize with 4% sodium hypochlorite solution for 30 minutes. Rinse with water 5-6 times.
[0033] (2) Cut open the sterilized fruit, take out the seeds, and cut off the seed wings with a sterile scalpel to expose the seeds. Inoculate into 1 / 2MS basic medium containing 0.5g / L activated carbon, cultivate in dark for about 1-2 weeks, the seeds begin to germinate seedlings, and then transfer to culture under lig...
Embodiment 2
[0040] The tissue culture rapid propagation method of embodiment 2 Lagerstroemia yunnanensis
[0041] (1) From November to December, when the seeds of Lagerstroemia yunnanensis are ripe, harvest the lignified but uncracked fruits, bring them back to the laboratory, and store them in a refrigerator at 4°C for 2 weeks. Soak in 100 times diluted detergent spirit for 30 minutes, use a cleaning ball to grind off the peel, then rinse under running water for more than 2 hours, sterilize with 75% alcohol in an ultra-clean workbench for 1 minute, and sterilize with 4% sodium hypochlorite solution for 30 minutes. Rinse with water 5-6 times.
[0042] (2) Cut open the sterilized fruit, take out the seeds, and cut off the seed wings with a sterile scalpel to expose the seeds. Inoculate into 1 / 2MS basic medium containing 0.5g / L activated carbon, culture in dark for about 1-2 weeks, the seeds begin to germinate seedlings, and then transfer to light culture.
[0043] The basic medium is: 1 / ...
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Abstract
The invention relates to a tissue culture rapid propagation method for Lagerstroemia intermedia Koehne. The method comprises the following steps: picking up lignified and uncracked fruits in the seed maturation season of Lagerstroemia intermedia Koehne, storing the fruits for 2 weeks under the condition of 4 DEG C, sterilizing the whole fruits, splitting the fruits, taking out the seeds, removing seed wings, inoculating a 1 / 2MS minimal medium containing 0.5g / L of activated carbon, performing dark culture for 1-2 weeks until the seeds germinate, and culturing in the sun; inoculating a multiple-shoot induction culture medium with germinated seedlings of which the height exceeds 2cm, and culturing for 5-6 weeks, thereby obtaining lots of multiple shoots; and inoculating a rooting medium with the multiple shoots, culturing for 6-8 weeks until all sterile seedlings root, hardening the rooting seedlings, and transplanting out of the bottle. Starting from propagation of Lagerstroemia intermedia Koehne, the problems that seed germination is difficult and the propagation coefficient is low under natural conditions are solved by utilizing a tissue culture method, and a foundation is laid for preservation of germplasm resources of Lagerstroemia intermedia Koehne and deeper research.
Description
technical field [0001] The invention relates to plant tissue culture technology, in particular to a tissue culture and rapid propagation method of Lagerstroemia yunnanensis. Background technique [0002] Yunnan crape myrtle (Lagerstroemia intermedia Koehne) belongs to the Lythraceae family. Lagerstroemia intermedia Koehne is a large evergreen tree and is a national third-level protected plant. At present, there are about 55 species of Lagerstroemia in the world, mainly distributed in eastern Asia, southeast and northern Australia. There are 18 species of Lagerstroemia native to my country, and 3 species were introduced and cultivated from Southeast Asia, totaling 21 species. Compared with other flowers and trees, Lagerstroemia genus has bright and beautiful flowers, long flowering period and long life span. In addition, it also has the advantages of strong anti-pollution ability and adaptability to urban environment. Therefore, it is deeply loved by people and widely used in...
Claims
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