A kind of rock shoot tissue culture seedling raising method
A technology of tissue culture and rock bamboo shoots, applied in the field of rock bamboo shoots tissue culture and seedling raising, can solve the problems of difficult restoration of wild resources, damage to the natural environment, small and large seeds, etc., and achieve the goal of solving difficult reproduction, fast reproduction speed and high reproduction coefficient Effect
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[0015] The invention is further described below in conjunction with specific embodiments:
[0016] A method for raising seedlings by tissue culture of rock bamboo shoots, comprising the following steps:
[0017] (1) Medium preparation: The medium includes germination induction medium, proliferation medium and rooting medium; among them, the germination induction medium is MS+NAA0.2mg / L+KT0.5mg / L+Huabao No.1 0.7g / L L+banana mash 30g / L+mashed potato 20g / L+sucrose 30g / L+agar 5g / L+PH5.9; proliferation medium is MS+IBA0.5mg / L+6-BA0.5mg / L+sucrose 30g / L+agar 5g / L+PH5.9; rooting medium is 2 / 3MS+NAA0.8mg / L+IAA0.5mg / L+sucrose 30g / L+activated carbon 0.5g / L+agar 5g / L+ph5.9; 60ml per bottle of culture medium and 120ml of rooting medium per bottle are packed into culture bottles, sterilized at 121°C and 0.5Mpa for 25min, cooled and solidified for later use.
[0018] (2) Preparation of explants: in autumn, pick plump fruits from mature plants as explants, and clean and disinfect them. The...
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