A kind of cultivation method of noble rot grape
A technology of noble rot grapes and cultivation methods, applied in the field of viticulture, can solve the problems that Botrytis cinerea does not have the ability to infect mature grapes, and the air humidity is stable and low, so as to increase the probability of infecting grapes, increase yield, and reduce planting rate. The effect of environmental requirements
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Embodiment 1
[0033] 1) Preparation of Botrytis cinerea strains
[0034] A. Seed culture medium: 200g of potatoes are crushed to small pieces with a diameter of no more than 1cm, 200g of barley malt is crushed to a particle size of about 20 mesh, added to 1L of tap water, saccharified at 60°C for 8 hours, filtered through a 40-mesh filter or sieve to remove residue, and the filtrate Make up to 1L volume with tap water, and sterilize with damp heat at 121°C for 20 minutes;
[0035] B. Bacterial strain: select Botrytis cinerea ACCC36257 preserved by the China Agricultural Microorganism Culture Collection Management Center;
[0036] C, fermentation: inoculation fermentation scrapes Botrytis cinerea spores or mycelium from the original seed slope, and the prepared biomass is 10 8 The original seed suspension of CFU / mL is inoculated in the seed medium at a volume ratio of 10%, and fermented in a shaker flask at 20°C, or fermented in a mechanically stirred fermenter for 10 days until the cell we...
Embodiment 2
[0045] 1) Preparation of Botrytis cinerea strains
[0046] A. Seed culture medium: 200g of potatoes are crushed to small pieces with a diameter of no more than 1cm, 200g of barley malt is crushed to a particle size of about 20 mesh, added to 1L of tap water, saccharified at 60°C for 8 hours, filtered through a 40-mesh filter or sieve to remove residue, and the filtrate Make up to 1L volume with tap water, and sterilize with moist heat at 121°C for 20min. The amount of preparation of seed culture medium can be enlarged in proportion.
[0047] B. Bacterial strain: Botrytis cinerea ACCC36258 preserved by the China Agricultural Microorganism Culture Collection Management Center is selected as the fermentation strain;
[0048] C, fermentation: inoculation fermentation scrapes Botrytis cinerea spores or mycelium from the original seed slope, and the prepared biomass is 10 6 The original seed suspension of CFU / mL was inoculated in the seed medium at a volume ratio of 4%, and fermen...
Embodiment 3
[0057] 1) Preparation of Botrytis cinerea strains
[0058] A. Seed culture medium: crush 300g of potatoes to small pieces with a diameter of no more than 1cm, crush 300g of barley malt to a particle size of about 20 mesh, add 1.5L of tap water, saccharify at 60°C for 8 hours, filter through a 40-mesh filter or sieve to remove residue, The filtrate was made up to a volume of 1.5 L with tap water, and sterilized by moist heat at 120°C for 20 minutes. The amount of seed culture medium can be expanded proportionally;
[0059] B. Bacterial strain: Botrytis cinerea ACCC36258 preserved by the China Agricultural Microorganism Culture Collection Management Center is selected as the fermentation strain;
[0060] C, fermentation: inoculation fermentation scrapes Botrytis cinerea spores or mycelium from the original seed slope, and the prepared biomass is 10 7 The original seed suspension of CFU / mL was inoculated in the seed medium at a volume ratio of 8%, and fermented in a shaking fla...
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