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Method for soaking seeds by epibrassinolide to relieve harm of different stresses on corn

A technology of epibrassinolide and adversity stress, which is applied in the field of alleviating corn seed germination and seedling growth, and can solve problems such as the rare application of hormone alleviating adversity stress

Active Publication Date: 2015-02-11
安徽英诺管理咨询有限公司
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  • Application Information

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Problems solved by technology

[0003] There have been a lot of reports on the prevention and treatment methods of corn salt damage and low temperature damage, most of which are based on physical methods, and the methods of hormones to alleviate adversity stress are rarely used in actual agricultural production

Method used

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  • Method for soaking seeds by epibrassinolide to relieve harm of different stresses on corn
  • Method for soaking seeds by epibrassinolide to relieve harm of different stresses on corn
  • Method for soaking seeds by epibrassinolide to relieve harm of different stresses on corn

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experiment example

[0023] Experimental example, 24-epibrassinolide seed soaking is carried out according to the following steps to the screening method of the optimum EBR concentration under two kinds of different adversity stresses:

[0024] 1. Material preparation: The seeds of corn inbred line TS141 were prepared with 24-epi-rapisenolide.

[0025] 2. Seed disinfection: select undamaged seeds of the same size, sterilize with 0.5% sodium hypochlorite for 10 minutes, rinse with distilled water for 3-5 times, and dry the attached water with filter paper.

[0026] 3. Seed soaking: (1) Seed soaking under salt stress: After the seeds are sterilized, put the seeds in distilled water, 180mmol / L NaCl, 180mmol / L NaCl+0.025mg / L EBR, 180mmol / L NaCl+0.050mg / L EBR, 180mmol / L·NaCl+0.100mg / L·EBR, 180mmol / L NaCl+0.200mg / L·EBR solution for 12h. (2) Seed soaking under low temperature stress: soak the seeds in distilled water, 0.001 mg / L, 0.010 mg / L, 0.050 mg / L, 0.100 mg / L, 1.000 mg / L EBR solutions for 12 h. S...

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Abstract

The invention belongs to the technical scheme of agricultural application, and particularly relates to a method for soaking seeds by exogenous 24-epibrassinolide to relieve harm of salt and low temperature different stresses on corn. The method comprises the following steps of disinfecting the seeds and performing salt stress of EBR (epibrassinolide)+NaCl solution in different concentrations and low temperature stress of EBR solution in different concentrations; performing germinating and seedling growing experiments. According to the method, the influence of the on germination and seedling growth of corn seeds exogenous EBR under the salt and low temperature stresses is respectively discussed, and the EBR is reflected to have an effect of relieving the harm on the seeds and seedlings under two different stresses. An exogenous EBR seed soaking method is expected to become a good method for respectively improving tolerance of the corn under salt stress and low temperature stress in practice, and the method is great in application potential; the method has high application value for relieving the harm of stresses on plants.

Description

technical field [0001] The invention belongs to the technical field of agricultural applications, and in particular relates to a method for alleviating corn seed germination and seedling growth under two different adversity stresses of salt and low temperature by exogenous 24-epibrassinolide seed soaking. Background technique [0002] Soil salinization and low temperature damage are common natural disasters in agricultural production, and they are serious problems in agricultural production and ecological environment in my country. Soil salinization refers to the process in which the salt in the bottom soil or groundwater rises to the surface with capillary water, and after the water evaporates, the salt accumulates in the surface soil. There are approximately 831 million hm worldwide 2 of the soil is threatened by salinization, of which the total area of ​​saline soil in my country is about 36 million hm 2 , accounting for 4.88% of the country's usable land area. Low tem...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08
Inventor 彭云玲闫慧萍赵小强武博洋
Owner 安徽英诺管理咨询有限公司
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