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A technology of flow cytometer and liquid flow, applied in the field of flow cytometer, can solve the problems of inability to guarantee cytometer and high detection accuracy, and achieve the goal of ensuring purity, high detection accuracy, improving detection accuracy and instrument stability Effect
Inactive Publication Date: 2015-03-25
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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[0003] In order to solve the problem that the existing cytometer liquid flow system cannot ensure that the cytometer has high detection accuracy under the conditions of low sample injection rate and high sample injection rate, the invention provides a flow cytometer liquid flow system. system
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[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0027] Such as figure 1As shown, the liquid flow system of a flow cytometer of the present invention includes a flow chamber, a fluid pump, a liquid flow pipeline, a solenoid valve, a pressure relief valve, a reversing valve, a pulsation buffer 23, an air bubble detection module 24, a ventilator Module 26, air filter 27, flushing buffer 35, sample injection tube 47, bead injection tube 48, waste liquid reservoir 49, sheath fluid reservoir 50, cleaning fluid reservoir 51, sanitizer reservoir 52, and defoaming fluid reservoir 53.
[0028] Such as figure 2 As shown, the flow chamber is composed of the entrance area 1, the convergence area 2, the adjustment area 3, the detection area 4 and the auxiliary area 5, and the entrance area 1, the convergence area 2, the adjustment area 3, the detection area 4 and the auxiliary area 5 are closely connected in sequence...
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Abstract
The invention discloses a liquid flow system of a flow cytometer, relates to the field of flow cytometers. The liquid flow system is used for solving the problems that the liquid flow system of the existing cytometer cannot have high detecting precision under low sampling velocity and high sampling velocity of the cytometers. The liquid flow system comprises two auxiliary sheath fluid pumps, a sample pump, a micro-bead pump, a primary sheath fluid, a flushing pump, four solenoid valves, five pressure relief valves, five filters, six reversing valves, a plurality of tee joints, a flow chamber, a pulsation damper, a gas bubble detecting module, a ventilating module, an air filter, a flushing buffer region, a sample inlet tube, a micro-bead sample inlet tube, a liquid waste tank, a sheath fluid tank, a cleanout fluid tank, a disinfectant tank and a gas bubble removing tank. The liquid flow system has relatively high detecting precision under a low sampling rate and a high sampling rate of the cytometer, has functions of cleaning, disinfecting and removing gas bubbles, not only can be used for ensuring the purity of different samples in the liquid flow system, but also can be used for improving the fluid stability and the linearity when a sample fluid passes through a laser checking area.
Description
technical field [0001] The invention relates to the technical field of flow cytometer, in particular to a liquid flow system of the flow cytometer. Background technique [0002] Flow cytometry can perform multi-parameter, quantitative analysis or sorting of cells or biological particles in a fast-flowing state. It has been widely used in basic research such as cell biology, immunology, physiology, and molecular biology. It is used in medical clinical diagnosis, environmental detection and so on. The liquid flow system is one of the three core systems of the flow cytometer. Its main function is to arrange the cells or other particles in the sample suspension in a single row and pass through the laser detection area sequentially and orderly. The liquid flow system mainly includes two parts, the flow chamber and the liquid path. The flow chamber uses the principle of hydrodynamic focusing to converge the sheath liquid and the sample liquid in the flow chamber, so that the samp...
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
Inventor 高庆嘉孙强赵建曾琪峰
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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