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A technology of flow cytometer and liquid flow, which is applied in the field of flow cytometer, can solve the problems of high detection accuracy and inability to guarantee the cytometer, achieve high detection accuracy, ensure purity, improve detection accuracy and instrument stability Effect
Inactive Publication Date: 2017-04-05
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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[0003] In order to solve the problem that the existing cytometer liquid flow system cannot ensure that the cytometer has high detection accuracy under the conditions of low sample injection rate and high sample injection rate, the invention provides a flow cytometer liquid flow system. system
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[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0027] Such as figure 1As shown, the liquid flow system of a flow cytometer of the present invention includes a flow chamber, a fluid pump, a liquid flow pipeline, a solenoid valve, a pressure relief valve, a reversing valve, a pulsation buffer 23, an air bubble detection module 24, a ventilator Module 26, air filter 27, flushing buffer 35, sample injection tube 47, bead injection tube 48, waste liquid reservoir 49, sheath fluid reservoir 50, cleaning fluid reservoir 51, sanitizer reservoir 52, and defoaming fluid reservoir 53.
[0028] Such as figure 2 As shown, the flow chamber is composed of the entrance area 1, the convergence area 2, the adjustment area 3, the detection area 4 and the auxiliary area 5, and the entrance area 1, the convergence area 2, the adjustment area 3, the detection area 4 and the auxiliary area 5 are closely connected in sequence...
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Abstract
A liquid flow system of a flow cytometer, relating to the field of flow cytometers, which solves the problem that the liquid flow system of the existing cytometer cannot guarantee the performance of the cytometer under the conditions of low sample injection rate and high sample injection rate. The problem of high detection accuracy. Includes two auxiliary sheath pumps, sample pump, bead pump, main sheath pump, wash pump, four solenoid valves, five pressure relief valves, five filters, six reversing valves, multiple tees, Flow Cell, Pulsation Buffer, Bubble Detection Module, Vent Module, Air Filter, Rinse Buffer, Sample Injection Line, Bead Injection Line, Waste Reservoir, Sheath Reservoir, Wash Reservoir, Disinfectant Reservoir, and Detox Reservoir Bubble pool. The invention ensures that the cytometer has high detection accuracy at both low sample injection rate and high sample injection rate, and has the functions of cleaning, disinfection and debubbling, which not only ensures the purity of different samples in the liquid flow system, In addition, the fluid stability and linearity of the sample liquid passing through the laser inspection area are improved.
Description
technical field [0001] The invention relates to the technical field of flow cytometer, in particular to a liquid flow system of the flow cytometer. Background technique [0002] Flow cytometry can perform multi-parameter, quantitative analysis or sorting of cells or biological particles in a fast-flowing state. It has been widely used in basic research such as cell biology, immunology, physiology, and molecular biology. It is used in medical clinical diagnosis, environmental detection and so on. The liquid flow system is one of the three core systems of the flow cytometer. Its main function is to arrange the cells or other particles in the sample suspension in a single row and pass through the laser detection area sequentially and orderly. The liquid flow system mainly includes two parts, the flow chamber and the liquid path. The flow chamber uses the principle of hydrodynamic focusing to converge the sheath liquid and the sample liquid in the flow chamber, so that the samp...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14
Inventor 高庆嘉孙强赵建曾琪峰
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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