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Primer combination and application thereof

A technology of primer composition and primer set, applied in combinatorial chemistry, bioreactor/fermenter combination, biological material pretreatment, etc., can solve the problem that the identification method of mycobacteria needs to be improved, etc., and is suitable for large-scale application and cost-effective. High and reduced workload

Inactive Publication Date: 2015-04-22
BGI GENOMICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, current methods for the identification of mycobacteria still need to be improved

Method used

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  • Primer combination and application thereof
  • Primer combination and application thereof
  • Primer combination and application thereof

Examples

Experimental program
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Embodiment 1

[0086] refer to figure 1 and figure 2 , according to the method for strain identification of mycobacteria to be tested of the present invention, utilize Sanger sequencing platform, carry out strain identification to 14 clinical sputum samples (this sample has been identified the mycobacterium type of infection by the method for bacterial strain cultivation )details as follows:

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Abstract

The invention provides a primer combination and an application thereof. The primer combination comprises a first primer group and a second primer group, wherein the nucleotide sequence of the first primer group is shown in the SEQ ID NO:1-2, and the nucleotide sequence of the second primer group is shown in the SEQ ID NO:3-4. The primer combination can specifically recognize the mycobacteria specific area-ITS area, has high homology between different mycobacteria, and is applicable to various mycobacteria and capable of being effectively used for recognizing the mycobacteria, particularly tuberculous mycobacteria and non-tuberculous mycobacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the technical field of mycobacterium identification. Specifically, it relates to a primer composition and its use. More specifically, the present invention relates to a primer composition, a kit and its use in the identification of mycobacteria to be tested, and to construct a nucleic acid sequencing library for the ITS region of mycobacteria to be tested. The method, the method and the system for identifying the strains of mycobacteria to be tested, and the method and system for identifying the strains of multiple mycobacteria to be tested simultaneously. Background technique [0002] Mycobacteria are a genus of aerobic intracellular bacterial organisms that survive in the endosomal compartments of monocytes and macrophages after infection of a host. Mycobacterium tuberculosis (Mycobacterium tuberculosis) is a member of the genus Mycobacterium (mycobacterium) in Gram-positive bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C40B50/06C12Q1/68C12Q1/04C12M1/34C12R1/32
Inventor 赵艳敏王晓宏
Owner BGI GENOMICS CO LTD
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