Temperature-controlled circulation device and gel electrophoresis system comprising the device

Abstract

The invention relates to a temperature control cycle device, which includes a water pump, a water inlet pipe, a water outlet pipe, a transformer, an AC relay, a water temperature control switch, a water pump switch, a water temperature control switch, a water level control switch, a water flow sensing switch, a water pipe temperature control switch, and a water temperature control switch. Control switch, core protection switch, overheating protection switch, heating power indicator light and heating coil; the invention also relates to a standardized gel electrophoresis system with a safety temperature control device, the system includes an electrophoresis box and a temperature control circulation device, the electrophoresis The inner bottom plate of the box is provided with several card slots, and the rubber plate bracket is fixed in the electrophoresis box through the card slots. There are several wire slots on the top of the electrophoresis box, and the electrophoresis line is passed through the wire slots. A cover plate is provided, and a temperature control circulation device is provided on one side of the electrophoresis box. By adopting the temperature-controlled circulation device of the present invention and the gel electrophoresis system including the device, the consistency of the electrophoresis conditions of the gel electrophoresis can be realized, and the temperature of the electrophoresis liquid can be ensured to be suitable.

Description

technical field [0001] The invention relates to the field of equipment control, in particular to biological technology, in particular to a temperature-controlled circulation device and a gel electrophoresis system comprising the device. Background technique [0002] Nucleic acid mutation detection technology (DGGE) is an electrophoretic detection technology commonly used in molecular biology. DGGE is an electrophoretic technology that separates DNA with different sequences through the difference in the concentration gradient of denaturant in polyacrylamide gel to achieve the purpose of detection. . The principle of this method is that according to the melting characteristics of DNA, the denaturing agent concentration required for the denaturation of the DNA double helix with different base compositions is different. The corresponding DNA is melted and denatured, resulting in a greatly reduced electrophoretic migration rate and stagnation on the gel. Different DNA molecules...

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Problems solved by technology

However, the above-mentioned invention does not solve the above-mentioned problems we raised. The electrophoresis tank can only electrophoresis one offset plate at a time. If there are many samples, batches of electrophoresis may also cause errors; design

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