A kind of breast cancer miRNA detection kit and the application of miRNA thereof

Abstract

The invention relates to application of combined utilization of miR-21, miR-191 and miR-16 as a breast cancer molecular marker, relates to application of the combined utilization of miR-21, miR-191, miR-16 and miR-39 in preparation of a breast cancer miRNA detection kit, and relates to the breast cancer miRNA detection kit. An RCR reaction system comprises a forward primer and a backward universal primer which respectively aim at miR-21, miR-191 and miR-16; an internal reference system comprises internal reference miRNA-39 and a forward prime which aims at miR-39; the nucleotide sequence of the forward primer aiming at miR-21 is shown in SEQ ID No.1; the nucleotide sequence of the forward primer aiming at miR-191 is shown in SEQ ID No.2; the nucleotide sequence of the forward primer aiming at miR-16 is shown in SEQ ID No.3; the nucleotide sequence of the forward primer aiming at miR-39 is shown in SEQ ID No.4; the nucleotide sequence of the backward universal primer is shown in SEQ ID No.5. The breast cancer miRNA detection kit disclosed by the invention is rapid and convenient in detection and high in accuracy, and can be used for assisting early diagnosis of the breast cancer.

Description

technical field [0001] The invention relates to a miRNA detection product and the application of miR-21, miR-191 and miR-16 as breast cancer molecular markers, belonging to the field of biotechnology. Background technique [0002] Breast cancer is the most common malignant tumor in women. There are about 2 million new breast cancer patients in the world every year. In recent years, the incidence of breast cancer in my country has increased rapidly, 1-2 percentage points higher than that in developed countries. traits such as genetic susceptibility. Breast cancer is clinically asymptomatic in the early stage, and the occult stage lasts an average of 12 years. When there are obvious symptoms, it is mostly in the middle and late stages. [0003] The application of molecular indicators to early detection of tumors is a current research hotspot, and microRNA (microRNA, miRNA) as a new generation of tumor markers for early diagnosis has attracted more and more attention in clinica...

AI Technical Summary

Problems solved by technology

[0004] The content of miRNA in serum is much lower than that in tissues, so a very sensitive and specific detection method is required; and because of the short sequence of miRNA primers, primer design is difficult, and no targeted software can be used directly, so both primers and systems need to be screened and tested. optimization process

At present, chip technology is generally used for high-throughput screening of miRNA, which has high cost, limited specificity and sensitivity, and cannot meet the needs of real-time detection of scattered samples; real-time detection of a small number of miRNAs mostly uses Taqman probe method, and some of them have been commercialized Kit products, but the cost and selling price are high

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