Application of PRKCZ autoantibody in auxiliary diagnosis of esophageal squamous carcinoma

A technology for auxiliary diagnosis, esophageal squamous cell carcinoma, applied in the field of medicine and biology

Active Publication Date: 2021-11-30
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no specific related antigen for esophageal squamous cell carcinoma

Method used

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  • Application of PRKCZ autoantibody in auxiliary diagnosis of esophageal squamous carcinoma
  • Application of PRKCZ autoantibody in auxiliary diagnosis of esophageal squamous carcinoma
  • Application of PRKCZ autoantibody in auxiliary diagnosis of esophageal squamous carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Using human proteome chips to screen markers for the diagnosis of esophageal squamous cell carcinoma

[0037] 1. Experimental sample:

[0038] The sera of 30 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) and 24 normal subjects (normal control group) were collected from the specimen bank of the Henan Provincial Key Laboratory of Cancer Epidemiology; among them, the serum of 30 patients with esophageal squamous cell carcinoma came from Patients with esophageal squamous cell carcinoma confirmed by pathology without any treatment; 24 cases of healthy control serum were obtained from healthy subjects, and the inclusion criteria of healthy subjects were: no cardiovascular, respiratory, liver, kidney, gastrointestinal tract, Endocrine, blood, mental, or nervous system diseases and the history of the above diseases, no acute or chronic diseases, no autoimmune diseases, no evidence of any tumor-related; moreover, 30 patien...

Embodiment 2

[0057] Example 2: ELISA detection of serum expression levels of autoantibodies against tumor-associated antigen PRKCZ

[0058] Enzyme linked immunosorbent assay (ELISA) was used to detect the expression level of autoantibodies against the tumor-associated antigen PRKCZ in the serum of a large sample of people.

[0059] 1. Experimental sample:

[0060] Sera from 90 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) and 90 healthy controls (healthy control group) were included in this study. Among them, the serum of 90 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) came from patients with esophageal squamous cell carcinoma in the First Affiliated Hospital of Zhengzhou University and Henan Cancer Hospital from 2017 to 2018. All cases were confirmed by histopathology without any surgery and radiotherapy and chemotherapy; 90 cases of healthy control serum (healthy control group) were obtained fro...

Embodiment 3

[0092] Example 3: The ability of autoantibodies against tumor-associated antigen PRKCZ to distinguish patients with esophageal squamous cell carcinoma, healthy controls and benign esophageal diseases

[0093] In order to further verify the ability of autoantibodies against the tumor-associated antigen PRKCZ to distinguish patients with esophageal squamous cell carcinoma, healthy controls, and patients with benign esophageal diseases, the present invention uses ELISA to detect the expression level of autoantibodies against the tumor-associated antigen PRKCZ in the serum of a large sample population

[0094] 1. Experimental sample:

[0095] This study included 126 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group), 126 healthy controls (healthy control group) and 237 patients with benign esophageal disease (esophageal benign disease group). Among them, the serum of 126 patients with esophageal squamous cell carcinoma (esophageal squamous ...

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Abstract

The invention belongs to the technical field of medical biology, and particularly discloses a biomarker for auxiliary diagnosis of esophageal squamous carcinoma and a detection kit. The biomarker for auxiliary diagnosis of esophageal squamous cell carcinoma provided by the invention is an autoantibody of an anti-tumor associated antigen PRKCZ, the expression level of the marker in serum of a patient with esophageal squamous cell carcinoma is higher than that of a normal person, and the difference has statistical significance. The invention also provides a kit for auxiliary diagnosis of esophageal squamous carcinoma, the kit contains a reagent for detecting the marker, and the reagent is used for detecting the biomarker in a sample through enzyme-linked immunosorbent assay, a protein chip, immunoblotting or microfluidic immunoassay. By detecting the expression level of the anti-tumor associated antigen PRKCZ in human serum, patients with esophageal squamous carcinoma and normal people can be effectively distinguished, and the kit can be used for auxiliary diagnosis of esophageal squamous carcinoma.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically discloses the application of PRKCZ autoantibody in auxiliary diagnosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is one of the most common malignant tumors in the world, and its morbidity and mortality are relatively high. The cancer statistics released in 2020 show that the incidence of esophageal cancer is higher in Asia, especially in East Asia, and men are higher than women. In East Asia, China's 2018 cancer statistics report shows that the number of new cases of esophageal cancer is about 324,000, accounting for about 50% of the world; the number of deaths is 301,000, accounting for about 55% of the world, which is enough to illustrate The prevalence of esophageal cancer in my country has intensified, and the prevention and treatment of esophageal cancer is urgent. The onset of esophageal cancer is insidious and ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573G01N33/543
CPCG01N33/57407G01N33/57488G01N33/573G01N33/543
Inventor 史健翔王科妍韩卓易春程王鹏叶华代丽萍
Owner ZHENGZHOU UNIV
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