A kind of lung cancer miRNA detection kit and the application of miRNA thereof

Abstract

The invention relates to a combination of miR-21, miR-24, miR-125b and miR-126 used as a lung cancer molecular marker, relates to application of the combination of miR-21, miR-24, miR-125b, miR-126 and miR-39 to the preparation of miRNA detection kit for lung cancers, and relates to a miRNA detection kit for the lung cancer. An RCR reaction system comprises positive primers and a reverse universal primer aiming at miR-21, miR-24, miR-125b and miR-126; an internal reference system comprises an internal reference miRNA-39 and a positive primer aiming at miR-39; a nucleotide sequence of the positive primer aiming at r miR-21 is shown in SEQ ID No.1; a nucleotide sequence of the positive primer aiming at miR-24 is shown in SEQ ID No.2; a nucleotide sequence of the positive primer aiming at miR-125b is shown in SEQ ID No.3; a nucleotide sequence of the positive primer aiming at miR-126 is shown in SEQ ID No.4; a nucleotide sequence of the positive primer aiming at miR-39 is shown in SEQ ID No.5; a nucleotide sequence of the reverse universal primer is shown in SEQ ID No.6. The miRNA detection kit for lung cancer is high in detection speed, convenient and high in correction rate and can assist in early diagnosis of lung cancer.

Description

technical field [0001] The invention relates to a miRNA detection product and the application of miR-21, miR-24, miR-125b and miR-126 as lung cancer molecular markers, and belongs to the field of biotechnology. Background technique [0002] About 1.3 million people die from lung cancer every year in the world, accounting for about 1 / 3 of all cancer deaths in the world. In my country, the number of deaths due to lung cancer exceeds 20% of the number of deaths due to tumors. Lung cancer patients are mostly in the middle and late stage when they have symptoms, and their 5-year survival rate is about 15%. If patients can be diagnosed in the early stage of lung cancer, their survival rate will be greatly improved. The occurrence and development of lung cancer is a process involving multiple genes, and the study of its molecular mechanism has positive significance for clinical diagnosis. Studies have found that microRNA (microRNA, miRNA) is related to the occurrence of various t...

AI Technical Summary

Problems solved by technology

The content of miRNA in serum is much lower than that in tissues, so a very sensitive and specific detection method is required; and because of the short sequence of miRNA primers, primer design is difficult, and no targeted software can be used directly, so both primers and systems need to be screened and tested. optimization process

At present, chip technology is generally used for high-throughput screening of miRNA, which has high cost, limited specificity and sensitivity, and cannot meet the needs of real-time detection of scattered samples; real-time detection of a small number of miRNAs mostly uses Taqman probe method, and some of them have been commercialized Kit products, but the cost and selling price are high

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